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This study was aimed at differentiating parental genomes, examining intergenomic composition, and mapping mitotic metaphase chromosomes by localizing parental and 18S rDNA probes in seven interspeci c hybrid progenies that originated from Lilium longi orum. Since in situ hybridization has not been previously used in lily breeding, ow cytometry was used in conjunction with genomic and uorescent in situ hybridization to determine the genomic contribution of each parent to the interspeci c progenies. A signi cant variation was observed in the DNA content, chromosome length, and 18S loci in F 1 as compared to the female and male parents. L. longi orum showed nearly two times higher DNA content than the male parents and L. longi orum × Asiatic progenies, but eight times higher than L. longi orum × L. hansonii. Genomic in situ hybridization results revealed that both female and male parents contributed an equal number of chromosomes to their interspeci c F 1 offspring. Fluorescent in situ hybridization mapping revealed that 18S rDNA had 8, 6 and 7 loci in L. longi orum parents, i.e., White heaven, Bright tower, and White tower, respectively, whereas each Asiatic cultivar and L. hansonii used as male showed 8 and 12 loci respectively. Interspeci c progenies showed 8 and 7 loci in LA, and 10-11 in LM hybrids. These cytogenetic results implied equal genetic and chromosomal contribution from both parents to their intergenomic progenies. Therefore, this combined (Schwarzacher et al., 1992)cytogenetic method has the potential to be an affordable and time-saving approach in lily breeding that could determine the status of hybrids and their genomic origin while achieving physical mapping and detecting genes in different genomes.

The major purpose of this paper was to have an understanding on rural women's awareness on agricultural extension services. Furthermore, the study aimed at exploring relationships between the selected characteristics of the rural women and their awareness on agricultural extension services. The study was conducted in a village of Singier upazila,Manikgonj and 125 rural women were selected as sample from the list of 502 housewives of the village. Data were collected from the selected rural women through using a pre-tested structured personal interview schedule during 16-28 February 2014. The majority of the rural women (56 percent) had medium awareness on agricultural extension activities, while 43 percent and only one percent had high and low awareness, respectively. Education, cosmopoliteness and extension contact of the rural women were positively correlated with their awareness on agricultural extension services, while family size was negatively correlated with their awareness. The rest of the variables, namely age, training exposure. Participation in farming activities, social participation, and role in decision making of the rural women showed no relationship with their awareness on agricultural extension services.

This study was aimed at differentiating parental genomes, examining intergenomic composition, and mapping mitotic metaphase chromosomes by localizing parental and 18S rDNA probes in seven interspecific hybrid progenies that originated from Lilium longiflorum. Since in situ hybridization has not been previously used in lily breeding, flow cytometry was used in conjunction with genomic and fluorescent in situ hybridization to determine the genomic contribution of each parent to the interspecific progenies. A significant variation was observed in the DNA content, chromosome length, and 18S loci in F1 as compared to the female and male parents. L. longiflorum showed nearly two times higher DNA content than the male parents and L. longiflorum × Asiatic progenies, but eight times higher than L. longiflorum × L. hansonii. Genomic in situ hybridization results revealed that both female and male parents contributed an equal number of chromosomes to their interspecific F1 offspring. Fluorescent in situ hybridization mapping revealed that 18S rDNA had 8, 6 and 7 loci in L. longiflorum parents, i.e., White heaven, Bright tower, and White tower, respectively, whereas each Asiatic cultivar and L. hansonii used as male showed 8 and 12 loci respectively. Interspecific progenies showed 8 and 7 loci in LA, and 10–11 in LM hybrids. These cytogenetic results implied equal genetic and chromosomal contribution from both parents to their intergenomic progenies. Therefore, this combined (Schwarzacher et al., 1992)cytogenetic method has the potential to be an affordable and time-saving approach in lily breeding that could determine the status of hybrids and their genomic origin while achieving physical mapping and detecting genes in different genomes.