Investigation of the morphological and cytogenetic variations of an intraspecific Asiatic lily hybrid using 5S and 18S rDNA probes

Mazharul, Islam Md; Yesmin, Reshma; Jung, Min-Jung; Kim, Hong-Yul; Kim, Chang Kil; Lim, Ki Byung

doi:10.1007/s13580-019-00216-7

Cited by 11 publications

(8 citation statements)

References 32 publications

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“…FISH and GISH have often been used to identify information surrounding chromosomal mutations, structure, and genomic evolution [ 99 , 100 ]. Oligos specific to a repetitive sequence or a particular genomic region can be visualized using fluorescence in situ hybridization [ 8 , 101 , 102 , 103 ]. For example, different ribosomal DNA (rDNA) signals are doubled in a tetraploid compared to those in a diploid.…”

Section: Cytogenetic Evaluation Of Induced Polyploidsmentioning

confidence: 99%

“…Aneuploid genomes have incomplete chromosome sets, which occur naturally in plant populations and are induced by chemical and physical agents [ 4 , 5 , 6 ]. Differences in the number of chromosomes [ 7 , 8 , 9 ] and phenotypic differences [ 10 , 11 , 12 ] have been verified using molecular cytogenetic methods. These differences increase allergic diversity [ 13 ] and heterozygosity [ 14 ] by genome buffering, resulting in novel dose effects.…”

Section: Introductionmentioning

confidence: 99%

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Cytogenetics and Consequences of Polyploidization on Different Biotic-Abiotic Stress Tolerance and the Potential Mechanisms Involved

Mazharul

Deepo

Nasif

et al. 2022

Plants

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The application of polyploidy in sustainable agriculture has already brought much appreciation among researchers. Polyploidy may occur naturally or can be induced in the laboratory using chemical or gaseous agents and results in complete chromosome nondisjunction. This comprehensive review described the potential of polyploidization on plants, especially its role in crop improvement for enhanced production and host-plant resistance development against pests and diseases. An in-depth investigation on techniques used in the induction of polyploidy, cytogenetic evaluation methods of different ploidy levels, application, and current research trends is also presented. Ongoing research has mainly aimed to bring the recurrence in polyploidy, which is usually detected by flow cytometry, chromosome counting, and cytogenetic techniques such as fluorescent in situ hybridization (FISH) and genomic in situ hybridization (GISH). Polyploidy can bring about positive consequences in the growth and yield attributes of crops, making them more tolerant to abiotic and biotic stresses. However, the unexpected change in chromosome set and lack of knowledge on the mechanism of stress alleviation is hindering the application of polyploidy on a large scale. Moreover, a lack of cost–benefit analysis and knowledge gaps on the socio-economic implication are predominant. Further research on polyploidy coupling with modern genomic technologies will help to bring real-world market prospects in the era of changing climate. This review on polyploidy provides a solid foundation to do next-generation research on crop improvement.

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Section: Cytogenetic Evaluation Of Induced Polyploidsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Cytogenetics and Consequences of Polyploidization on Different Biotic-Abiotic Stress Tolerance and the Potential Mechanisms Involved

Mazharul

Deepo

Nasif

et al. 2022

Plants

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show abstract

“…Fluorescent in situ hybridization (FISH) is an advanced technique used to detect DNA sequences and allele copy numbers in different positions on chromosomes (Ramzan et al, 2017;Jo et al, 2019;Islam et al, 2020). Analysis of nuclear organizing regions can be achievable using FISH technique (Fujiwara et al, 2007;Hwang et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Chromosome characterization and physical mapping of 18S rDNA in Lilium longiflorum originated interspecific hybrids using combined genomic and fluorescent in situ hybridization

Islam

Lee²,

Deepo³

et al. 2022

Euphytica

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This study was aimed at differentiating parental genomes, examining intergenomic composition, and mapping mitotic metaphase chromosomes by localizing parental and 18S rDNA probes in seven interspeci c hybrid progenies that originated from Lilium longi orum. Since in situ hybridization has not been previously used in lily breeding, ow cytometry was used in conjunction with genomic and uorescent in situ hybridization to determine the genomic contribution of each parent to the interspeci c progenies. A signi cant variation was observed in the DNA content, chromosome length, and 18S loci in F 1 as compared to the female and male parents. L. longi orum showed nearly two times higher DNA content than the male parents and L. longi orum × Asiatic progenies, but eight times higher than L. longi orum × L. hansonii. Genomic in situ hybridization results revealed that both female and male parents contributed an equal number of chromosomes to their interspeci c F 1 offspring. Fluorescent in situ hybridization mapping revealed that 18S rDNA had 8, 6 and 7 loci in L. longi orum parents, i.e., White heaven, Bright tower, and White tower, respectively, whereas each Asiatic cultivar and L. hansonii used as male showed 8 and 12 loci respectively. Interspeci c progenies showed 8 and 7 loci in LA, and 10-11 in LM hybrids. These cytogenetic results implied equal genetic and chromosomal contribution from both parents to their intergenomic progenies. Therefore, this combined (Schwarzacher et al., 1992)cytogenetic method has the potential to be an affordable and time-saving approach in lily breeding that could determine the status of hybrids and their genomic origin while achieving physical mapping and detecting genes in different genomes.

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“…For example, flow cytometry offers a quick, precise and simple way to determine nuclear DNA content (C-value) (Galbraith et al, 2009). In addition, fluorescence in situ hybridization (FISH) and karyotyping are commonly used techniques for cytogenetic studies (Anamthawat-Jónsson, 2004;Hwang et al, 2020;Islam et al, 2020). FISH analysis may provide basic details about each species' ploidy degree and chromosomal characteristics.…”

Section: Introductionmentioning

confidence: 99%

Induction of Polyploidy in Cucumis melo ‘Chammel’ and Evaluation of Morphological and Cytogenetic Changes

Cho¹,

Deepo²,

Mazharul³

et al. 2021

HST

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This study explored the effects of oryzalin on the polyploidization of a new cultivar, Cucumis melo 'Chammel'. 58 diploid seedlings of 'Chammel' were treated with oryzalin. Their ploidy levels were checked by flow cytometry and number of 5S and 18S rDNA loci were examined by fluorescence in situ hybridization (FISH). Twelve among 58 plants were identified as tetraploids. The tetraploids were self-crossed, and their seeds were harvested. The seeds and seedlings of the tetraploids were compared to the corresponding diploids using morphology and cytogenetic analyses. The seed width, the cotyledon width, and the leaf thickness of the tetraploids increased significantly over those of the diploids. However, the length of the petiole of the tetraploids was shorter than that of the corresponding diploids. The stomata length and width of the guard cells in the tetraploids were longer than in diploids, but the number of stomata per area decreased in the tetraploids. The number of chromosomes was 24 and 48 for the diploids and tetraploids, respectively. Moreover, the chromosomal changes induced by oryzalin were confirmed; the diploids had one pair of 5S rDNA loci and two pairs of 18S rDNA loci, the corresponding numbers were doubled in the tetraploids. These results will help to generate new seedless oriental melon cultivars via polyploidization.

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Investigation of the morphological and cytogenetic variations of an intraspecific Asiatic lily hybrid using 5S and 18S rDNA probes

Cited by 11 publications

References 32 publications

Cytogenetics and Consequences of Polyploidization on Different Biotic-Abiotic Stress Tolerance and the Potential Mechanisms Involved

Cytogenetics and Consequences of Polyploidization on Different Biotic-Abiotic Stress Tolerance and the Potential Mechanisms Involved

Chromosome characterization and physical mapping of 18S rDNA in Lilium longiflorum originated interspecific hybrids using combined genomic and fluorescent in situ hybridization

Induction of Polyploidy in Cucumis melo ‘Chammel’ and Evaluation of Morphological and Cytogenetic Changes

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