Cytogenetic assignment of 53 microsatellites from the USDA-MARC porcine genetic map

Abstract

This study provides 53 new fluorescent in situ hybridization cytogenetic assignments for microsatellite markers linked on the swine genetic map. Forty microsatellites are physically assigned for the first time. The chromosomal locations of eight markers were either confirmed or refined, while five loci were assigned to locations different from those given in previous reports. Markers were selected to provide physical anchors based on their presumed proximity to centromeres or telomeres and at approximately 30 cM intervals across the genetic map. The number of physical anchors for pig (SSC) chromosomes 8, 15, and 18 linkage groups was significantly improved. Centromeric regions were localized to areas less than 10 cM for SSC 1, 2, 3, 6, 7, 8, and 9. Although the recombination rate was generally higher across small biarmed chromosomes and lowest for large acrocentric chromosomes, two regions with particularly low (1q2.1→q2.9 and 13q2.3→q4.1) and three regions with extremely high (5p1.5→p1.2, 6p1.4→p1.3, and 12p1.5→p1.4) rates of recombination were detected. These assignments represent an overall 10% increase in the number of physically assigned markers in Sus scrofa and more than a 20% increase in the number of Type II loci assigned to the pig cytogenetic map.

Assignment of swine TNFRSF6 (Fas) to chromosome 14q25→q27 and TNFSF6 (FasL) to chromosome 9q14→q22 by FISH and radiation hybrid mapping

“…Swine chromosome ideograms are shown beside the RH map. The correlation of the locations of TNFRSF6, TNFSF6 and the markers that are nearest to TNFRSF6 and TNFSF6 among those already mapped in both the cytogenetic map and the IMpRH map (SW2593 and SW2515 for TNFRSF6 and S0095 and SW1651 for TNFSF6 [Ellegren, 1993;Rohrer et al, 1996;Lopez-Corrales et al, 1999]) between the RH map and the cytogenetic map was also indicated in this figure to support the correctness of the location mapped based on our results.…”

Mapping and microsatellite marker development for the porcine leukemia inhibitory factor receptor (LIFR) and epidermal growth factor receptor (EGFR) genes

“…Two-point analysis revealed close linkage of the EGFR gene to the microsatellites SW749 at a distance of 6.7 cR (LOD score 5.47) and SW1651 at a distance of 8.2 cR (LOD score 4.00). SW1651 has been physically assigned to SSC9q26 (Lopez-Corrales et al, 1999), which is in perfect accordance with the FISH result.…”

Microsatellite-based characterization of Southern African domestic pigs (<i>Sus scrofa domestica</i>)

“…A panel of 39 microsatellite loci distributed throughout the genome and identified as part of the Pig Genome Project was used in this study. The markers used (with chromosome position) were: S0073 (4), SW35 (4), S0298 (16), SW1134 (5), SW1851 (1), SW2456 (X/Y), SW2514 (2), SW983 (9), S0120 (18), SW2 (5), SW1557 (14), SW378 (5), SW761 (14), SW2008 (11), SW995 (5), SW352 (7), SW472 (7), SW949 (X/Y), S0004 (15), S0165 (3), S0217 (4), SW225 (13), SW1041 (10), SW21 (9), SW2404 (4), S0035 (6), S0006 (16), SW749 (9), SW2410 (8), SW940 (9), S0295 (9), SW839 (4), SW2406 (6), SW2419 (6), SW316 (6), S0121 (7), SW322 (6), S0385 (11) and SW2443 (2) (Fredholm et al, 1993;Ellegren et al, 1994;Hoyheim et al, 1994;McQueen et al, 1994;Robic et al, 1994;Rohrer et al, 1994;Groenen et al, 1995;Alexander et al, 1996;Rohrer et al, 1996;Lopez-Corrales et al, 1999).…”