Cytogenetic comparisons between A and G genomes in Oryza using genomic in situ hybridization

Xiong, Zhiyong; Tan, Guang Xuan; He, Guangyuan; He, Guang Cun; Song, Yun

doi:10.1038/sj.cr.7310033

Cited by 15 publications

(10 citation statements)

References 30 publications

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“…Over the evolutionary long term, there have arisen different degrees of genetic differences among the various genomes and even different chromosomes within the same genome of the genus Oryza, and the GISH method has been widely used to study this differentiation and the resulting relationships [26][27][28][29] . In the present study, a two-step in situ hybridization procedure including GISH and FISH was used to discriminate among the A, C, and D genomes in the interspecific hybrid between O. sativa and O. latifolia and to investigate chromosome pairing at meiotic metaphase I in the pollen mother cells of the hybrid.…”

Section: Discussionmentioning

confidence: 99%

“…In the present study, the crossability of the hybrid between O. sativa and O. latifolia was 0-4.89%. However, with the development of embryo rescue techniques, an increasing number of interspecific hybrids between O. sativa and wild rice species with different genomes has been produced, including O. latifolia (CCDD genome) [19] , O. minuta (BBCC genome) [20] , O. eichingeri (CC genome) [13] , O. officinalis (CC genome) [10,21] , O. australiensis (EE genome) [22,23] , O. brachyantha (FF genome) [24] , O. ridleyi (HHJJ genome) [25] , and O. meyeriana (GG genome) [26] . The obtained interspecific hybrids offer an opportunity to transfer useful genes from wild rice species to commercially cultivated varieties.…”

Section: Discussionmentioning

confidence: 99%

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Development and characterization of interspecific hybrids between Oryza sativa and O. latifolia by in situ hybridization

Tang

Zhou

et al. 2008

Sci. Bull.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Development and characterization of interspecific hybrids between Oryza sativa and O. latifolia by in situ hybridization

Tang

Zhou

et al. 2008

Sci. Bull.

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“…The simultaneous addition of more than one probe to the hybridization mix is called the multicolor GISH (mGISH) technique (Li et al, 2001a,b;Xiong et al, 2006). Even at low stringency levels, mGISH showed good resolution in the karyotypic characterization of species of complex genomic constitution, such as allotetraploids (Li et al, 2001a,b).…”

Section: Discussionmentioning

confidence: 99%

Establishment of the genomic in situ hybridization (GISH) technique for analysis in interspecific hybrids of Passiflora

Melo¹,

Silva²,

Souza³

2015

Genet. Mol. Res.

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ABSTRACT. The genomic in situ hybridization (GISH) technique was applied to Passiflora interspecific F 1 HD13-133 hybrids (Passiflora sublanceolata x Passiflora foetida) and HD15-101 (Passiflora gardineri x Passiflora gibertii), and the backcrossed hybrids (BC 1 ) HD18-106 and HD18-113 (Passiflora sublanceolata x HD13-133). GISH was performed using genomic probes prepared with the DNA from the paternal genitor, whereas the maternal DNA was used as blocking DNA and employed at various concentrations (20X, 40X, 60X, and 100X) in relation to the probe concentration. At the same time, GISH was applied with the use of simultaneous probes from both genomes, paternal and maternal, that were detected with avidin-FITC and antidigoxigenin-rhodamine, respectively. Both methodologies allowed the distinguishing of the maternal and paternal genomes, thus confirming the hybrid nature of all the analyzed genotypes. Furthermore, the presence of recombinant chromosomes in BC 1 hybrids revealed the occurrence of meiotic recombination in HD13 hybrids. This application 2177 ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (1): 2176-2188 (2015 GISH technique for Passiflora of the GISH technique is an important step towards genomic analyses of Passiflora hybrids: it can broaden the phylogenetic and evolutionary studies of the genus and, at the same time, contribute to breeding programs.

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“…on MI cells but some reports on the extent of synapsis at the pachytene stage can also be found (Xiong et al, 2006). Traditional dyes or modern equivalents like DAPI are the easiest and less time-consuming tools for the purpose when both parental species are diploids because the chromosomes in the hybrid have only the choice of allosyndetic (crop-wild) pairing.…”

Section: Potential Of Crop-to-wild Genetic Transfermentioning

confidence: 99%

“…Thinopyrum bessarabicum hybrids (King et al, 1993). Nowadays, this cytomolecular tool has become the most extended method for MI pairing analyses of interspecific hybrids not only from wheat (e.g., Benavente et al, 1998;Jauhar et al, 2004) but from many other crop species as distinct as tomato (Ji and Chetelat, 2003), onion (Stevenson et al, 1998), barley (Zhang et al, 1999), maize (Gonzalez et al, 2006), dahlia (Gatt et al, 2000), lily (Lim et al, 2001), rice (Xiong et al, 2006) and several brassicas (Wang et al, 2004;Wei et al, 2006), among others ( Figs. 4, 8).…”

Section: Potential Of Crop-to-wild Genetic Transfermentioning

confidence: 99%

The use of cytogenetic tools for studies in the crop-to-wild gene transfer scenario

Benavente

Cifuentes

Dusautoir³

et al. 2008

Cytogenet Genome Res

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Interspecific hybridization in plants is an important evolutionary phenomenon involved in the dynamics of speciation that receives increasing interest in the context of possible gene escapes from transgenic crop varieties. Crops are able to cross-pollinate with a number of wild related species and exchange chromosome segments through homoeologous recombination. In this paper, we review a set of cytogenetic techniques that are appropriate to document the different steps required for the stable introgression of a chromosome segment from a donor species (i.e., the crop) into a recipient species (i.e., the wild). Several examples in hybrids and derivatives are given to illustrate how these approaches may be used to evaluate the potential for gene transfer between crops and wild relatives. Different techniques, from classical chromosome staining methods to recent developments in molecular cytogenetics, can be used to differentiate genomes and identify the chromosome regions eventually involved in genetic exchanges. Some clues are also given for the study of fertility restoration in the interspecific hybrid forms.

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Cytogenetic comparisons between A and G genomes in Oryza using genomic in situ hybridization

Cited by 15 publications

References 30 publications

Development and characterization of interspecific hybrids between Oryza sativa and O. latifolia by in situ hybridization

Development and characterization of interspecific hybrids between Oryza sativa and O. latifolia by in situ hybridization

Establishment of the genomic in situ hybridization (GISH) technique for analysis in interspecific hybrids of Passiflora

The use of cytogenetic tools for studies in the crop-to-wild gene transfer scenario

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