Cytogenetic mapping of eight genes encoding fatty acid binding proteins (FABPs) in the pig genome

Abstract

In the present study cytogenetic localization of eight fatty acid binding protein genes in the pig genome was shown. BAC clones, containing sequences of selected genes (FABP1, FABP2, FABP3, FABP4, FABP5, FABP6, FABP7 and FABP8) were derived from porcine BAC libraries and mapped by FISH to porcine chromosomes (SSC) 3q12, 8q25, 6q26, 4q12, 4q12, 16q22, 1p22 and 4q12, respectively. Detailed analyses of regions containing gene clusters (FABP4, FABP5, FABP8) in chromosome 4 were performed and their order was established. It was shown that these three genes are located beyond the FAT1 region. Assignment of the FABP genes to chromosome regions harboring quantitative trait loci (QTL) for fat deposition is discussed.

A note on associations between polymorphism within the 2,4-dienoyl-CoA reductase gene (<i>DECR1</i>) and growth rate of Polish Landrace boars

“…The inconsistency may be due to differences in population origin and size as well as the statistical model applied (GLM vs haplotype contrasts). Among other possible candidate genes located within QTL interval 71-86cM on the long arm of SSC4, a polymorphism within the FABP4 gene (adipocyte fatty acid binding protein) mapped physically at position 4q12 (Szczerbal et al, 2007) revealed no association with fatness traits in Polish Landrace pigs (Chmurzyńska et al, 2008). Our current fi ndings support the hypothesis of the more general role of DECR1 V54L polymorphism, namely its participation in biosynthetic processes also expressed in growth regulation.…”

Regulatory role of Sirt1 on the gene expression of fatty acid‐binding protein 3 in cultured porcine adipocytes

“…Together, these results demonstrated that Sirt1 could downregulate FABP3 gene expression in porcine adipocytes, suggesting that FABP3 and Sirt1 are directly involved in adipocyte function. FABPs are abundant intracellular proteins that play important roles in intracellular fatty acid transport, regulation of gene expression and cell growth [Haunerland and Spener, 2004;Chmurzynska, 2006;Szczerbal et al, 2007]. The FABPs have unique tissue-specific distributions and many FABPs are prominently expressed in a single tissue or cell type.…”

“…Numerous functions have been proposed, including cellular uptake and transport of fatty acids (FAs), targeting of FAs to specific metabolic pathways, participation in gene expression regulation and cell growth [Haunerland and Spener, 2004;Szczerbal et al, 2007]. At this point in time, nine tissue-specific cytoplasmic FABPs have been identified and their tertiary structures have proven to be highly conserved [Chmurzynska, 2006;Furuhashi and Hotamisligil, 2008;Storch and Corsico, 2008].…”

Identification of target sequences for association studies - analysis of the pig <i>FABP3</i> and <i>FABP4</i> loci using comparative genomics methods

“…Good functional candidates are FABP loci which encode proteins involved in the transmembrane and intracellular fatty acid movement (Zimmerman and Veerkamp, 2002). The porcine FABP4 is localized in 4q12 and H-FABP in 6q26, in chromosomal regions where QTLs for fatness traits were found (Szczerbal et al, 2007).…”