Regulatory role of Sirt1 on the gene expression of fatty acid‐binding protein 3 in cultured porcine adipocytes

Shan, Tizhong; Ren, Yanling; Wu, Ting; Liu, Chen X.; Wang, Yi Z.

doi:10.1002/jcb.22203

Cited by 21 publications

(14 citation statements)

References 46 publications

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“…The FABP3 , C/EBP and PPAR families are crucial for fat differentiation [66], [67]. We found that CEBPD and CEBPG highly expressed at 2 dpn of both breeds and FABP3 highly expressed at 2 and 28 dpn, while PPARA and PPARG expressed at very low levels in skeletal muscle (Figure 4).…”

Section: Discussionmentioning

confidence: 76%

Comparative Analyses by Sequencing of Transcriptomes during Skeletal Muscle Development between Pig Breeds Differing in Muscle Growth Rate and Fatness

et al. 2011

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Understanding the dynamics of muscle transcriptome during development and between breeds differing in muscle growth is necessary to uncover the complex mechanism underlying muscle development. Herein, we present the first transcriptome-wide longissimus dorsi muscle development research concerning Lantang (LT, obese) and Landrace (LR, lean) pig breeds during 10 time-points from 35 days-post-coitus (dpc) to 180 days-post-natum (dpn) using Solexa/Illumina's Genome Analyzer. The data demonstrated that myogenesis was almost completed before 77 dpc, but the muscle phenotypes were still changed from 77 dpc to 28 dpn. Comparative analysis of the two breeds suggested that myogenesis started earlier but progressed more slowly in LT than in LR, the stages ranging from 49 dpc to 77 dpc are critical for formation of different muscle phenotypes. 595 differentially expressed myogenesis genes were identified, and their roles in myogenesis were discussed. Furthermore, GSK3B, IKBKB, ACVR1, ITGA and STMN1 might contribute to later myogenesis and more muscle fibers in LR than LT. Some myogenesis inhibitors (ID1, ID2, CABIN1, MSTN, SMAD4, CTNNA1, NOTCH2, GPC3 and HMOX1) were higher expressed in LT than in LR, which might contribute to more slow muscle differentiation in LT than in LR. We also identified several genes which might contribute to intramuscular adipose differentiation. Most important, we further proposed a novel model in which MyoD and MEF2A controls the balance between intramuscular adipogenesis and myogenesis by regulating CEBP family; Myf5 and MEF2C are essential during the whole myogenesis process while MEF2D affects muscle growth and maturation. The MRFs and MEF2 families are also critical for the phenotypic differences between the two pig breeds. Overall, this study contributes to elucidating the mechanism underlying muscle development, which could provide valuable information for pig meat quality improvement.The raw data have been submitted to Gene Expression Omnibus (GEO) under series GSE25406.

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Section: Discussionmentioning

confidence: 76%

Comparative Analyses by Sequencing of Transcriptomes during Skeletal Muscle Development between Pig Breeds Differing in Muscle Growth Rate and Fatness

et al. 2011

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“…At d30, genes related to adipose deposition were clearly more active in Jinhua than in Landrace pigs (Jinhua-up genes) (Table 4). These include stearoyl-CoA desaturase (NM_213781.1), acetyl-Coenzyme A acyltransferase 1 (CK455955), lipoprotein lipase (BF712908) [33]–[35], hormone-sensitive lipase (AY686758.1) [36]–[38], fatty acid synthase (CN166778) [39]–[41], fatty acid binding protein 3 (CB471223) [42]–[44], C1Q and collagen domain containing adiponectin (AY589691.1) [45] and 1-acylglycerol-3-phosphate O-acyltransferase 1 (BG608754) etc. At d90 and d150, more adipose deposition-related genes were classified as Jinhua-up genes, including caveolin 2 (BF191227) [46]–[48], C-4 to C-12 straight chain acyl-Coenzyme A dehydrogenase (NM_214039.1) [49], [50], lipoprotein lipase (AY686760.1) and 3-oxoacid CoA transferase 1 (NM_213938.1) [51] etc at d90 (Table 5), and solute carrier family 27 member 4 (fatty acid transporter) (CN156586), nitrilase 1 (BX672817) [52], ribosomal protein L32 (NM_001001636.1), ribosomal protein L23 (AJ296004) [53], ribosomal protein L12 (BP172489), claudin 7 (CK450245) and carboxylesterase (NM_214246.1) [54]–[56] etc at d150 (Table 6).…”

Section: Resultsmentioning

confidence: 99%

“…The number of intramuscular pre-adipocytes isolated in suspension was determined as described previously. The preadipocytes were seeded on six-well (35-mm) tissue culture plates in complete media (DMEM/F12+10% fetal bovine serum (FBS)+100 Upenicillin+100 Ustreptomycin) and cultured at 37°C under a humidified atmosphere of 95% air and 5% carbon dioxide according to previous study [42].…”

Section: Methodsmentioning

confidence: 99%

Distinctive Genes Determine Different Intramuscular Fat and Muscle Fiber Ratios of the longissimus dorsi Muscles in Jinhua and Landrace Pigs

Zhang

Yuan

et al. 2013

PLoS ONE

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Meat quality is determined by properties such as carcass color, tenderness and drip loss. These properties are closely associated with meat composition, which includes the types of muscle fiber and content of intramuscular fat (IMF). Muscle fibers are the main contributors to meat mass, while IMF not only contributes to the sensory properties but also to the plethora of physical, chemical and technological properties of meat. However, little is known about the molecular mechanisms that determine meat composition in different pig breeds. In this report we show that Jinhua pigs, a Chinese breed, contains much higher levels of IMF than do Landrace pigs, a Danish breed. We analyzed global gene expression profiles in the longissimus dorsi muscles in Jinhua and Landrace breeds at the ages of 30, 90 and 150 days. Cross-comparison analysis revealed that genes that regulate fatty acid biosynthesis (e.g., fatty acid synthase and stearoyl-CoA desaturase) are expressed at higher levels in Jinhua pigs whereas those that regulate myogenesis (e.g., myogenic factor 6 and forkhead box O1) are expressed at higher levels in Landrace pigs. Among those genes which are highly expressed in Jinhua pigs at 90 days (d90), we identified a novel gene porcine FLJ36031 (pFLJ), which functions as a positive regulator of fat deposition in cultured intramuscular adipocytes. In summary, our data showed that the up-regulation of fatty acid biosynthesis regulatory genes such as pFLJ and myogenesis inhibitory genes such as myostatin in the longissimus dorsi muscles of Jinhua pigs could explain why this local breed produces meat with high levels of IMF.

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“…In addition, it is reported that Sirt1 can also regulate the expression of FABP3 gene partly through the PPARc in pig adipocytes [21]. Taken together, we speculate that RXRa cooperated with PPARa to regulate the expression of FABP3 in muscle, while KLF15 and CREB interact with PPARa or PPARc to regulate the expression of FABP3 in adipocytes.…”

Section: Transcriptional Activity Of the Bovine Fabp3 Gene Promotermentioning

confidence: 90%

“…To date, there are many reports about the expression regulation of FABP3 [19][20][21][22]. However, the information about transcriptional regulation of FABP3 in cattle is limited.…”

Section: Introductionmentioning

confidence: 97%

Tissue expression analysis, cloning and characterization of the 5′-regulatory region of the bovine FABP3 gene

Wang

et al. 2016

Mol Biol Rep

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Fatty acid binding protein 3 (FABP3) is a member of the FABP family which bind fatty acids and have an important role in fatty acid metabolism. A large number of studies have shown that the genetic polymorphisms of FABP3 are positively correlated with intramuscular fat (IMF) content in domestic animals, however, the function and transcriptional characteristics of FABP3 in cattle remain unclear. Real-time PCR analysis revealed that bovine FABP3 was highly expressed in cardiac tissue. The 5'-regulatory region of bovine FABP3 was cloned and its transcription initiation sites were identified. Sequence analysis showed that many transcriptional factor binding sites including TATA-box and CCAAT-box were present on the 5'-flanking region of bovine FABP3, and four CpG islands were found on nucleotides from -891 to +118. Seven serial deletion constructs of the 5'-regulatory region evaluated in dual-luciferase reporter assay indicated that its core promoter was 384 base pairs upstream from the transcription initiation site. The transcriptional factor binding sites RXRα, KLF15, CREB and Sp1 were conserved in the core promoter of cattle, sheep, pigs and dogs. These results provide further understanding of the function and regulation mechanism of bovine FABP3.

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Regulatory role of Sirt1 on the gene expression of fatty acid‐binding protein 3 in cultured porcine adipocytes

Cited by 21 publications

References 46 publications

Comparative Analyses by Sequencing of Transcriptomes during Skeletal Muscle Development between Pig Breeds Differing in Muscle Growth Rate and Fatness

Comparative Analyses by Sequencing of Transcriptomes during Skeletal Muscle Development between Pig Breeds Differing in Muscle Growth Rate and Fatness

Distinctive Genes Determine Different Intramuscular Fat and Muscle Fiber Ratios of the longissimus dorsi Muscles in Jinhua and Landrace Pigs

Tissue expression analysis, cloning and characterization of the 5′-regulatory region of the bovine FABP3 gene

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