Cytogenetic Studies on Nine Genotypes of Phaseolus vulgaris 
 L. Cultivated in Egypt in Relation to Zinc Efficiency

Mekki, Laila; Badr, Abdelfattah; Fekry, M.

doi:10.3923/pjbs.2007.4230.4235

Cited by 5 publications

(8 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Castagnaro et al (1990) reported that the genome size of the wild common bean was 1.71 pg2C -1 , whereas the cultivated common bean had different values, such as 1.56, 1.63, 1.69, and 1.79 pg2C -1 . In another study involving 9 accessions, it was reported that the genome size of the common bean varied between 2.65 and 4.96 pg2C -1 (Mekki et al, 2007). It was clear that the results obtained from the current study were similar to some of the previous studies, while different from others.…”

Section: Resultssupporting

confidence: 66%

“…Today, the flow cytometry method is used to determine genome size; however, the genetic resources of the common bean have not been examined in detail using the flow cytometry method (FCM). To date, a very limited number of studies have been conducted, in which only a few common bean accessions coexisted or were analyzed with other species (Ayonoadu, 1974;Bennet et al, 1982;Castagnaro et al, 1990;Arumuganathan and Earle, 1991;Beletti et al, 1997;Mekki et al, 2007;Labotan et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Molecular cytogenetic characterization of common bean (Phaseolusvulgaris L.) accessions

Tuna¹,

Yücel²,

Aşçioğul³

et al. 2020

Turk J Agric For

View full text Add to dashboard Cite

With an annual global production of approximately 25 million tons, the common bean (Phaseolus vulgaris L), a member of the genus Phaseolus, is one of the major protein sources used as food for humans. In this study, it was aimed to investigate the genome size of the common bean genetic resource collection (154 common bean accessions) in Turkey by flow cytometry (FCM) and determine whether geographical variables affected the genome size. In addition, the number and distribution of 5S and 45S ribosomal DNA loci were designated by performing a fluorescence in situ hybridization (FISH) analysis in some of the accessions. The FCM analyses revealed that the mean nuclear DNA content of the accessions varied from 1.28 pg2C-1 to 1.55 pg2C-1 (mean 1.35 pg2C-1), and the differences between these accessions were statistically significant (P < 0.01). Intraspecific variation in the genome size was determined, and a positive correlation was found between the altitude and genome size. However, latitude and longitude did not have any statistically significant effect on the genome size. In the principal coordinate analysis, the accessions were divided into 3groups. Based on the results of the FISH analysis performed on 5 different accessions with varying genome sizes, using 5S and 45S rDNA genes as probes, the number of 5S rDNA loci was 4 in the common bean and stable among the common bean accessions, while the number of 45S rDNA loci was highly polymorphic, varying between 6 and 16. Consequently, it was determined in the present study that the genetic resource collection of common bean had a wide variation in terms of genome size and genome organization.

show abstract

Section: Resultssupporting

confidence: 66%

Section: Introductionmentioning

confidence: 99%

Molecular cytogenetic characterization of common bean (Phaseolusvulgaris L.) accessions

Tuna¹,

Yücel²,

Aşçioğul³

et al. 2020

Turk J Agric For

View full text Add to dashboard Cite

show abstract

“…The chromosome number in all genotypes was 2n = 2x = 22, confirming several previous results [17,[19][20][21][22][23][24][27][28][29][30]. The MTCL (Mean Total Chromosome Length) recorded in the studied genotypes varied from 15.65 ± 0.99 to 34.24 ± 0.35 micrometers (Table 2), which is almost similar to the previous study on nine genotypes of the common bean, who's MTCL was measured to be between 13.74 and 29.50 micrometers [24]. In another study on bean karyotype analysis, the MCI (Mean Centromere Index) ranged between 42 and 48, which is very similar to our results (43.18 ± 0.99 to 46.83 ± 3.52) (Table 2) [39].…”

Section: Discussionsupporting

confidence: 89%

“…In another study on bean karyotype analysis, the MCI (Mean Centromere Index) ranged between 42 and 48, which is very similar to our results (43.18 ± 0.99 to 46.83 ± 3.52) (Table 2) [39]. The MAR (Mean Arm Ratio) was calculated to be between 1.14 ± 0.10 and 1.36 ± 0.07 (Table 2) in this study, which does not correspond to previous results (3.50 ± 0.36 to 4.30 ± 0.35) [24]. The mean genome size of the common bean accessions varied between 1.49 ± 0.032 pg in the E.L. genotype and 3.28 ± 0.101 pg in the B.T.…”

Section: Discussionsupporting

confidence: 74%

“…Many classical and molecular cytogenetic studies have been performed on this plant, but since these characteristics are variable under the influence of geographical and ecological factors [15,16], it seems necessary to study the characteristics of the nuclear and chromosomal genomes separately in each region. Several cytogenetic studies have been conducted to investigate the chromosomal structure of the common bean, which include chromosome analysis and cytogenetic maps [17][18][19][20][21][22][23][24][25][26][27][28][29][30]. Genome size information is an important issue in ploidy analysis, genome analysis, taxonomy, evolution, and breeding studies [31][32][33][34][35].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Analysis of Nuclear DNA Content and Karyotype of Phaseolus vulgaris L.

Kulaz

Najafi

Tunçtürk

et al. 2022

Genes

View full text Add to dashboard Cite

The common bean (Phaseolus vulgaris L.), whose annual production is 26 million tons worldwide, is one of the main sources of protein and is known as one of the most important food sources. In this study, the karyotype variations and the genome size of four common bean genotypes in Turkey were investigated to determine whether the geographic variables in these regions affected the genome size and the karyotype parameters. In addition, it is known that as that the cytological and chromosomal parameters change under the influence of the climatic conditions of each region, appropriate and stable cytological methods for each plant facilitate and enable the determination of the chromosomal structure and the identification of specific chromosomes in the genotypes of the relevant region. Correct and valuable information such as this enables breeders and researchers to determine the correct shape and actual size of chromosomes. The genome size of the genotypes was measured with a flow cytometer, and chromosome analyses were performed with the squash method. For each genotype, the karyotype parameters, such as the number of somatic chromosomes, the Mean Total Chromosome Length (MTCL), the Mean Centromere Index (MCI), and the Mean Arm Ratio (MAR), were measured. The results showed that the highest and the lowest amounts of DNA per nucleus (3.28 pg and 1.49 pg) were observed in the Bitlis and Elaziğ genotypes. In addition, all genotype chromosome numbers were counted to be 2n = 2x = 22. The Mean Total Chromosome Length varied from 15.65 µm in Elaziğ to 34.24 µm in the Bitlis genotype. The Mean Chromosome Length ranged between 1.42 µm and 3.11 µm in the Elaziğ and Bitlis genotypes. The Hakkari and Van genotypes consist of eleven metacentric chromosomes, while the Bitlis and Elaziğ genotypes consist of ten metacentric chromosomes and one sub-metacentric chromosome. However, the Mean Centromere Index and Arm Ratio differed considerably among the genotypes. The highest (46.88) and the lowest (43.18) values of the Mean Centromere Index were observed in the Hakkari and Elaziğ genotypes, respectively. On the other hand, the lowest (1.15) and the highest (1.36) values of the Mean Arm Ratio were obtained in the Bitlis and Elaziğ genotypes, respectively. Eventually, intraspecies variations in genome size and chromosomal parameters were observed, and it was determined that the changes in nuclear DNA content and different chromosomal parameters among the four Phaseolus genotypes from four different regions of Turkey indicate the effect of climate change in the regions on these parameters. Such information in these areas can be used as useful information for the improvement of this plant and breeding programs.

show abstract

Morphological and rDNA fluorescence in situ hybridization analyses of chrysanthemum cultivars from Korea

Wang

Jung²,

Kim³

et al. 2021

Hortic. Environ. Biotechnol.

View full text Add to dashboard Cite

Cytogenetic Studies on Nine Genotypes of Phaseolus vulgaris L. Cultivated in Egypt in Relation to Zinc Efficiency

Cited by 5 publications

References 35 publications

Molecular cytogenetic characterization of common bean (Phaseolusvulgaris L.) accessions

Molecular cytogenetic characterization of common bean (Phaseolusvulgaris L.) accessions

Analysis of Nuclear DNA Content and Karyotype of Phaseolus vulgaris L.

Morphological and rDNA fluorescence in situ hybridization analyses of chrysanthemum cultivars from Korea

Contact Info

Product

Resources

About