Cytogenetic studies on submerged plants from the Yenisei River area in the zone of radioactive contamination

Муратова, Е. Н.; Goryachkina, Olga V.; Kornilova, M.; Pimenov, А. V.; Седельникова, Т. С.; Bolsunovsky, A. Ya.

doi:10.1134/s1062359014050094

Cited by 8 publications

(6 citation statements)

References 4 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…We did not manage to definitively identify dicentric chromosomes either in metaphase or in ana‐telophase of the Elodea cell cycle, nor were we able to exactly identify double bridges and double fragments. We observed a few ring structures in the present study and in Elodea roots previously collected from the radioactively contaminated part of the Yenisei River . However, we did not show enhanced levels of abnormal cells in Elodea in sediments with the highest concentrations of radionuclides (bottom sediment‐5), which could be considered as a dose‐dependent response.…”

Section: Discussioncontrasting

confidence: 65%

“…The Allium test is commonly used for testing natural soils and sediments . The number of chromosomes in the karyotype of E. canadensis is quite variable and much higher (2n = 24–96) compared with Allium cepa (2n = 16) , and this makes analysis of chromosome abnormalities more difficult. Chromosomes of E. canadensis (Figure ) are, however, not much smaller than chromosomes of onion (the width of chromosomes ranges from 0.75 µm to 1.0 µm for Elodea and 1.5 µm to 1.75 µm for onion); they are quite visible, allowing identification of unstable chromosome abnormalities (bridges, fragments, vagrant chromosomes, multipolar mitosis, and agglutination).…”

Section: Discussionmentioning

confidence: 99%

“…Elodea canadensis is widely used for monitoring anthropogenic contamination of freshwater ecosystems , including the Yenisei River , and is also used in bioassays . The sensitivity of E. canadensis to genotoxic xenobiotics deposited in bottom sediment of the Yenisei River was demonstrated in our previous studies . Hence, E. canadensis can be considered a potential indicator of genotoxicity in whole‐sediment tests.…”

Section: Introductionmentioning

confidence: 89%

“…A dose‐dependent increase in the incidence of chromosome abnormalities was reported in roots of aquatic plants from water bodies in the Chernobyl (Ukraine) exclusion zone . A high percentage of cells with chromosome abnormalities was recorded in roots of aquatic plants sampled in the radioactively and chemically contaminated sediments of the Yenisei River (Siberia, Russia) .…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Use of the aquatic plant Elodea canadensis to assess toxicity and genotoxicity of Yenisei River sediments

Зотина

Трофимова

Medvedeva

et al. 2015

Enviro Toxic and Chemistry

View full text Add to dashboard Cite

The toxicity, cytotoxicity, and genotoxicity of bulk sediments from the Yenisei River (Siberia, Russia) were estimated in laboratory bioassays based on several endpoints in the aquatic plant Elodea canadensis. The bottom sediment samples were collected in the Yenisei River upstream and downstream of the sources of chemical and radioactive contamination. The testing revealed different sensitivities of Elodea endpoints to the quality of the bottom sediment: weight of shoots < length of shoots < mitotic index < length of roots < percentage of abnormal cells. The response of the genotoxicity endpoint (percentage of cells with chromosome abnormalities in roots of Elodea) was the highest in sediments with chemical pollution, whereas the highest inhibition of toxicity endpoints (shoot and root length) occurred in sediments with the highest level of radioactive pollution. The extreme response of Elodea endpoints to the quality of certain sediment samples may be regarded as related to the possible presence of unknown toxicants. The results show that E. canadensis can be used as an indicator species in laboratory contact testing of bottom sediment. The responses of shoot and root length growth endpoints of Elodea can be recommended as basic sensitivity indicators of bottom sediment toxicity. Analysis of cells carrying abnormal chromosomes in the apical root meristem of Elodea can be performed optionally in the same test to assess the genotoxicity of sediments.

show abstract

Section: Discussioncontrasting

confidence: 65%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 89%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Use of the aquatic plant Elodea canadensis to assess toxicity and genotoxicity of Yenisei River sediments

Зотина

Трофимова

Medvedeva

et al. 2015

Enviro Toxic and Chemistry

View full text Add to dashboard Cite

show abstract

“…Радіаційно-індуковані в G 2 -періоді розриви хроматид утворюються в момент конденсації хроматину та формування хроматинового волокна (Parshad & Sanford, 2001;Magin et al, 2015;Guogyte et al, 2017). Мости з'являються як у результаті розриву та об'єднання розірваних кінців хромосом, так і внаслідок аглютинації (злипання хромосом) (Muratova et al, 2014;Albertson, 2015;Zotina et al, 2015;Pampalona et al, 2016). У працях інших дослідників також зазначається причетність районів із радіоактивним забрудненням до утворення подвійних і множинних дицентриків (Geras'kin et al, 2011;Firbas & Amon, 2014).…”

Section: обговоренняunclassified

Cytogenetic activity of radionuclide contamination of water reservoirs of the alienation zone of Chornobyl NPP

Yakymchuk

2018

Regul. Mech. Biosyst.

View full text Add to dashboard Cite

Thirty years after the accident, the alienation zone of Chornobyl NPP continues to be an open source of radionuclide spread which is carried with superficial and soil waters into river systems and moves beyond the area. The study of mutagenic activity of radionuclide contamination of the water reservoirs in the near zone of Chornobyl NNP will make it possible to predict genetic consequences of their effect in the years after the accident. The purpose of this research is to study frequency and spectrum of chromosome aberrations in root meristem cells of Triticum aestivum L. under the prolonged effect of radionuclide contamination of water and bottom deposits of the water reservoirs in the near alienation zone of Chornobyl NPP. Seeds of winter wheat varieties Al’batros odes’kyi and Zymoiarka were sprouted in the conditions of the effect of water radionuclide contamination of the Prypiat River, Brahinka River, a reservoir-cooler of ChNPP, Semyhodskyi backwater, drainage-way 3 of ChNPP, Lakes Hlyboke and Azbuchyn (total specific activity of 137Cs and 90Sr – 0.17–52.99 Bq/м3) and bottom deposits of the left and right banks of Prypiat canal, a reservoir-cooler of ChNPP, drainage-ways 1–3 of ChNPP (total specific activity of 137Cs and 90Sr – 16.0–45.0 Bq/kg). Frequency and spectrum of cytogenetic disorders were identified in the cells of root meristem sprouts with help of the ana-telophase method. Under the influence of radiation on water and bottom deposits of the water reservoirs in the alienation zone of ChNPP, a 1.6–4.2 times increase in the frequency of chromosome aberrations and mitosis disorders was found. The highest levels of cytogenetic activity were shown by water radionuclide contamination in a reservoir-cooler of ChNPP, Semyhodskyi backwater and bottom deposits of drainage-way 2. The correlation between frequency of chromosome aberrations and specific value of radionuclide activity of water reservoirs has not been recorded, which can prove the induction of cytogenetic disorders resulting from the radiation in the low-rate range. The spectrum of cytogenetic disorder types is mostly represented by acentric fragments, bridges and lagging chromosomes. The induction of the cells with lagging chromosomes, which exhibit the highest levels (0.24–0.38%), under the effect of radionuclide contamination of water in Hlyboke Lake, the Brahinka River, the Prypiat River, a reservoir-cooler of ChNPP and bottom deposits of drainage-way 3, allows one to assume the availability of aneugenic factors in the water reservoirs in the alienation zone of ChNPP. The water entities of the alienation zone of ChNPP, the level of radionuclide contamination of which is characterized by a high cytogenetic activity, induce cells with complex chromosome rearrangements of high frequency. Despite the decrease in chromosome aberration frequency effected by the water of the Prypiat River near Chornobyl city, the Brahinka River and bottom deposits of the right bank of Prypiat canal, the increased level of aneugenic cells and the induction of multiple chromosome rearrangements confirm the persistence of mutagenic activity in the abovementioned contaminated water entities.

show abstract

How can the natural radiation background affect DNA integrity in angiosperm plant species at different altitudes in Rila Mountain (Southwest Bulgaria)?

Jovtchev

Stankov

Ravnachka

et al. 2019

Environ Sci Pollut Res

View full text Add to dashboard Cite

Cytogenetic studies on submerged plants from the Yenisei River area in the zone of radioactive contamination

Cited by 8 publications

References 4 publications

Use of the aquatic plant Elodea canadensis to assess toxicity and genotoxicity of Yenisei River sediments

Use of the aquatic plant Elodea canadensis to assess toxicity and genotoxicity of Yenisei River sediments

Cytogenetic activity of radionuclide contamination of water reservoirs of the alienation zone of Chornobyl NPP

How can the natural radiation background affect DNA integrity in angiosperm plant species at different altitudes in Rila Mountain (Southwest Bulgaria)?

Contact Info

Product

Resources

About