Cytogenetic study of the pure Resveratrol extracted from grape skin on normal peripheral human blood lymphocytes

Mohammed, Zainab Y.; Al-Jumaily, Essam F.; Yaseen, Nahi Y.

doi:10.24126/jobrc.2011.5.2.156

Cited by 2 publications

(2 citation statements)

References 8 publications

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“…However, these values are all not significant compared to the negative control. Nevertheless, A. santolina was the best among the studied plants because it has the highest MI values at all concentrations used (3.3±0.28 at 1 mg/ml, 3±0.14 at 3 mg/ml 2.25±0.07 at 5 mg/ml) close to the normal MI Studies showed that the flavonoid and thymol decreased MI at a higher concentration, and also the pure resveratrol from grape skin antagonized the mitotic action of PHA and acted as an anti-mitotic agent 17, 18 . The extracts from three plants which are investigated in the current study contain flavonoids and tannins (Phytochemical screening), and the highest flavonoid content was observed in C. verutum 5 .…”

Section: Discussionmentioning

confidence: 90%

In Vitro Genotoxicity of Three Plants From Asteraceae Family in Human Lymphocytes Cultures

2022

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Background: Onopordum carduiforme, Centaurea verutum, and Achillea santolina are medicinal plants grown in Syria and commonly used in traditional medicine. Such as antibacterial, antioxidant and anticancer properties. However, the genotoxic effects of these plants have not been studied. Aim and objective: the aim of this study was to evaluate the genotoxic effects of hydroethanolic extracts of these plants on human lymphocyte cultures model by evaluating the cell proliferation, determination of mitotic index (MI), and their effects on chromosomes. Methods: the hydroethanolic extracts of the aerial parts of the three plants were extracted using an Ultrasonic bath. Then the genotoxic effects of hydroethanolic extracts of these plants on human lymphocyte cultures was conducting by determination of mitotic index (MI). Results: the results showed that all three plants decreased non-significantly the mean of mitotic index in comparison with negative control (normal MI) (p>0.05) at concentrations (1, 3, 5 mg/ml) and the mitotic index values ranged was between (2.25±0.07 and 3.3±0.28). However, C. verutum showed the lowest mitotic index (3±0.14 at 1 mg/ml) and (2.25±0.07 at 5 mg/ml), and did not induce chromatid or chromosome breaks or gaps. Conclusion: these preliminary results on cytotoxicity and mutagenicity of these plants provide valuable information about the safety of using them in alternative medicine. Peer Review History: Received: 11 November 2021; Revised: 13 December; Accepted: 28 December, Available online: 15 January 2022 Academic Editor: Dr. A.A. Mgbahurike, University of Port Harcourt, Nigeria, amaka_mgbahurike@yahoo.com UJPR follows the most transparent and toughest ‘Advanced OPEN peer review’ system. The identity of the authors and, reviewers will be known to each other. This transparent process will help to eradicate any possible malicious/purposeful interference by any person (publishing staff, reviewer, editor, author, etc) during peer review. As a result of this unique system, all reviewers will get their due recognition and respect, once their names are published in the papers. We expect that, by publishing peer review reports with published papers, will be helpful to many authors for drafting their article according to the specifications. Auhors will remove any error of their article and they will improve their article(s) according to the previous reports displayed with published article(s). The main purpose of it is ‘to improve the quality of a candidate manuscript’. Our reviewers check the ‘strength and weakness of a manuscript honestly’. There will increase in the perfection, and transparency. Received file: Reviewer's Comments: Average Peer review marks at initial stage: 6.0/10 Average Peer review marks at publication stage: 7.0/10 Reviewers: Ahmad Najib, Universitas Muslim Indonesia, Makassar, Indonesia, ahmad.najib@umi.ac.id Dr. Dennis Amaechi, MrsFoluBabade Mini Estate , Flat 5 by Old Soldiers Quarter, Sabongari/Bwari, Abuja- Federal Capital Territory, Nigeria. amaechitoexcel@yahoo.com Dr. Sangeetha Arullappan, Universiti Tunku Abdul Rahman, Malaysia, sangeetha@utar.edu.my Similar Articles: A STUDY ON DIFFERENT PLANTS OF APOCYNACEAE FAMILY AND THEIR MEDICINAL USES STUDY LITERATION OF CHEMICAL CONTENTS OF SOME PLANTS THAT POTENTIALLY AS THE SOLAR SOWS EXPLORING THE ANTIPARASITIC ACTIVITY OF MEDICINAL PLANTS

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Section: Discussionmentioning

confidence: 90%

In Vitro Genotoxicity of Three Plants From Asteraceae Family in Human Lymphocytes Cultures

2022

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“…Resveratrol may exert its anti-inflammatory effects by reducing the quantity and kind of leukocytes in BALF (Zhang et al, 2014) . Additionally, a cytogenetic analysis of resveratrol on healthy human blood lymphocytes showed a significant reduction of the mutagen's ability to induce mitosis (Mohammed et al, 2011). Previous published research demonstrated that resveratrol may ameliorate asthma by preventing the production of inflammatory mediators, increasing airway remodeling, and suppressing inflammation that is brought on by asthma (Alharris et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

Ovalbumin-Induced Asthma in Rats is Alleviated by Resveratrol Treatment

AL-Khaqani,

Mohammed

2024

JAHP

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The number of affected people by asthma is rising to reach up to 339 million worldwide. Asthma defined as chronic airway inflammation. A natural substance called resveratrol, which is present in several plants, red wine, and grapes, has been researched for its potential anti-inflammatory, and antioxidant qualities, and to induce relaxation of airway smooth muscle. The current study investigated whether Resveratrol (RES) could lessen allergic asthma that ovalbumin (OVA) causes in rats. Current results revealed that RES improved significantly (P<0.05) allergic asthma attenuation by decreasing the number of infiltrated mononuclear cells in Bronchoalveolar Lavage Fluid (BALF) and non-significantly (P>0.05) the number of infiltrated mononuclear cells intraperitoneally. RES increased lung integrity by reducing Evans blue extravasation in the lung (P<0.05) and liver (P>0.05). Additionally, in the BALF, RES therapy reduced (P<0.05) inflammatory cytokines including interleukin-17 (IL-17) and interferon-gamma (IFN-γ). RES increases (P<0.05) the expression of Il10 gene in the spleen, which is associated with T-regulatory and anti-inflammatory processes in the immune system. Our findings showed that RES may reduce asthma by activating anti-inflammatory genes, restoring lung barrier integrity, and decreasing proinflammatory cytokines and infiltrating inflammatory cells.

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Cytogenetic study of the pure Resveratrol extracted from grape skin on normal peripheral human blood lymphocytes

Cited by 2 publications

References 8 publications

In Vitro Genotoxicity of Three Plants From Asteraceae Family in Human Lymphocytes Cultures

In Vitro Genotoxicity of Three Plants From Asteraceae Family in Human Lymphocytes Cultures

Ovalbumin-Induced Asthma in Rats is Alleviated by Resveratrol Treatment

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