Cytogenetics of Clitoria. I. Induced autotetraploidy in C. ternatea.

Srivastav, P. K.; Raina, S. N.

doi:10.1508/cytologia.47.99

Cited by 5 publications

(5 citation statements)

References 8 publications

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“…Treatments with higher concentrations and longer durations proved to be deleterious. Similar results were reported by Srivastav and Raina (1982). Out of these 11 autotetraploid plants, only 4 plants could survive up to maturity.…”

Section: Methodssupporting

confidence: 86%

“…According to Kostaff (1940), large size of the chromosomes have direct bearing on multivalent frequency. However, Morrison and Rajhathy (1960a) and Srivastav and Raina (1982) suggested that multivalent frequency in autotetraploids was largely dependent on short size of chromosomes. Present studies indicate that apart from chromosome size, other factors such as chiasma frequency which is under genetic control, heterozygous nature of plants, environmental conditions etc., might also be responsible for multivalent formation (Riley and Chapman 1958).…”

Section: Cytological Observationsmentioning

confidence: 99%

“…There are few reports on chromosome number, karyomorphology and meiotic chromosome behaviour in this species (Fedorov 1969, Mathew and Ninan 1989, Gandhi and Patil 1993. Srivastav and Raina (1982) have obtained colchiploid autotetraploid plants in the same species. Present study deals with induction of autotetraploidy and morphological characters as well as chromosome behaviour in C. ternatea white.…”

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confidence: 97%

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Colchicine-Induced Autotetraploidy in Clitoria ternatea L.

Gandhi

Patil

1997

CYTOLOGIA

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Section: Methodssupporting

confidence: 86%

Section: Cytological Observationsmentioning

confidence: 99%

mentioning

confidence: 97%

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Colchicine-Induced Autotetraploidy in Clitoria ternatea L.

Gandhi

Patil

1997

CYTOLOGIA

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“…The induced colchitetraploids showed all the characteristics features of induced polyploidy. A slower rate of growth and development was pronounced at the early stages of colchitetraploid plants and this observation is supported by earlier reports (Srivastav and Raina 1982, 1987, Dibyendu 2010, Dar et al 2017, Verma et al 2017. This reduced rate of growth and development at the initial stage could be because of a reduced rate of mitotic cell division (Eigsti 1947), a lesser quantity of growth hormone (Avery and Pottorf 1945), or a slower rate of physiological and biochemical processes (Chen and Tang 1945).…”

Section: Discussionsupporting

confidence: 81%

Morphological and Chromosomal Characterization of Diploid and Colchicine Induced Autotetraploid Plants of <i>Commelina benghalensis</i> L. (Commelinaceae)

et al. 2021

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Induced polyploidy is well known to enhance the genetic architecture of plants that confers better survivorship to them in various agro-climatic conditions than their respective diploids. In this regard, the present study was aimed to obtain the autotetraploid of Commelina benghalensis through antimitotic agent colchicine in order to increase its commercial, ornamental, and medical value. For tetraploidy induction, ungerminated seeds and tips of cotyledon-stage seedlings of the plant were treated with various concentrations of colchicine (0.1, 0.15, 0.2, and 0.25%). Experiments carried out on ungerminated seeds were unsuccessful in generating stable polyploids as it causes the death of germinated seedlings at the very earliest stage. Conversely, applying aqueous colchicine solution constantly on tips of seedlings for 6-8 h per day for three consecutive days was found significant in producing stable tetraploids. Putative tetraploids were subjected to chromosomal, morphological, and palynological observations for their exact confirmation. Out of total 120 seedlings treated, six plants (5%) were found polyploid in C 0 generation with the highest frequency (10%) observed in 0.2% colchicine. During meiosis, the PMCs of tetraploid plants exhibited 44 chromosomes in different associations like quadrivalent, trivalent, bivalent and univalent, while that of the control diploid plants showed regular 11 bivalents. Meiotic analysis showed a constant decrease of quadrivalent frequency and the increasing number of bivalents from C 0 to C 2 for better fertility. Furthermore, differences in vegetative and ornamental characteristics between diploid and polyploid plants were significant. The size of pollen grains, capsules, and seeds were significantly greater than those of diploid plants.

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“…However, reduction in the height of the plant, number of branches, internodal distance and number of stomata per unit area indicates that the duplication of gene does not always increase in size but may also reduce it (Singh andRoy 1971, Srivastav andRaina 1982). While considering morphological effects in induced tetraploids it is often stated that one of the chief attributes of polyploidy is gigantism (Sastry et al 1968, Das et al 1970, Alekperova 1979.…”

Section: Cytologymentioning

confidence: 99%