Induced tetraploidy in mulberry I. Morphological, anatomical and cytological investigations in cultivar RFS-135.

Dwivedi, N. K.; Sikdar, A. K.; Dandin, S. B.; Sastry, C. R.; Jolly, M. S.

doi:10.1508/cytologia.51.393

Cited by 34 publications

(22 citation statements)

References 9 publications

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“…The presence of 14 bivalents in most of the PMCs at metaphase I confirms the diploid nature of the variant. One bivalent was found to be larger in comparison to the other, as also reported by Gill and Gupta (1979) and Dwivedi et al (1986) in Morus, and by Das (1961) in Morus indica.…”

Section: Resultssupporting

confidence: 85%

Cytogenetical Studies in Some Diploid Mulberry Varieties (Moraceae)

2013

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Summary Most Morus species are diploid, having 28 chromosomes, but a few species, specifically, M. tiliafolia Makino, Morus cathyana and M. nigra L., are higher polyploidy. In these species, the chromosome number varies from 2n=28 to 2n=308, with ploidy level from x to 22x. In the present study, three diploid mulberry varieties, namely, V 1 , S 13 and S 34, have been analyzed for detailed meiotic studies. Based on chromosome configuration and other meiotic behaviour, x=14 has been considered as the basic number of the genus. Meiosis shows marginal differences. Various meiotic abnormalities, such as the occurrence of one large chromosome at metaphase I, unequal separation at anaphase I, the presence of laggards and precocious movement of chromosomes at anaphase II, and two micronuclei at tetrad, have been observed. The low pollen fertility and seed sets may be due to irregular chromosomal pairing and separation rather than due to genetic or physiological causes.

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Section: Resultssupporting

confidence: 85%

Cytogenetical Studies in Some Diploid Mulberry Varieties (Moraceae)

2013

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“…Further lesser frequency per unit area is more suitable for rain fed conditions. However, reduction in the internodal and number of stomata per unit area indicates that the increased dosage of genes does not always increase in size but may also reduce it (Dwivedi et al, 1986).…”

Section: Variety Tr-10mentioning

confidence: 99%

Cytomorphological studies of two mulberry varieties (Moraceae)

Venkatesh¹,

Shivaswamy²,

Munirajappa³

2014

Afr. J. Biotechnol.

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Two mulberry varieties, namely, S 34 and Tr-10 were selected for cytomorphological studies. Stomatal frequency, somatic chromosome number, ploidy level and meiotic behaviour were studied for these varieties. S 34 is diploid with 2n=28 and Tr-10 is triploid with 2n=42 chromosomes. Meiosis was irregular. Various anomalies like univalents, bivalents, trivalents laggards, loose association and unequal separation of chromosomes were observed in some pollen mother cells (PMCs) studied. Stomatal frequency was found to be lesser in triploid when compared to diploid variety.

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“…These mutagenic chemicals disrupt mitosis by preventing microtubule polymerisation and, hence, the polar migration of chromosomes at anaphase. Colchicine is one of the most commonly used spindle inhibitors and has been used to good eVect in numerous plants (Hancock 1997), including some trees and other woody species (Dwivedi et al 1986;Roberts et al 1990;Rose et al 2000;Vainola 2000;Blakesley 2002;Kadota and Niimi 2002;Shao et al 2003). Here, we present the Wrst report of the successful induction of tetraploid plants of P. acerifolia and detail a methodology for the eYcient recovery of such polyploid plants through the in vivo application of colchicine.…”

Section: Introductionmentioning

confidence: 99%

Colchicine-induced chromosome doubling in Platanus acerifolia and its effect on plant morphology

Bao

2007

Euphytica

143

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We report the production of tetraploid plants of Platanus acerifolia, with the ultimate aim of improving the ornamental qualities of this important urban landscaping tree. Chromosome doubling was achieved by the application of colchicine to either pre-soaked seed or to the apical meristems of young seedlings. Treatment of the ungerminated seed was the more eYcient method in terms of numbers of tetraploid seedlings (up to 40%, as determined by chromosome counting of the root-tip nuclei) but this method produced no mature tetraploid plants due to the deleterious eVect of colchicine on subsequent root growth. When colchicine was applied directly to the apical growing tip of cotyledon-stage seedlings, leaf and stem growth was temporarily aVected but the plants eventually recovered. We conducted a preliminary screen for putative tetraploids based on the observation in other plant species of a correlation of stomatal size and distribution with ploidy. Plants containing signiWcantly larger stomata and at a lower density across the lower leaf epidermis, were selected for further analysis by Xow cytometry and chromosome counting. These techniques conWrmed that, of the 12 putative polyploids, four were tetraploid, Wve were mixoploid and three were, in fact, diploid. Morphological diVerences of the tetraploids included a more compact growth habit and broader, thicker leaves. These plants are being grown to full maturity in order to test their potential for use in a breeding programme aimed at producing sterile triploid lines.

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Induced tetraploidy in mulberry I. Morphological, anatomical and cytological investigations in cultivar RFS-135.

Cited by 34 publications

References 9 publications

Cytogenetical Studies in Some Diploid Mulberry Varieties (Moraceae)

Cytogenetical Studies in Some Diploid Mulberry Varieties (Moraceae)

Cytomorphological studies of two mulberry varieties (Moraceae)

Colchicine-induced chromosome doubling in Platanus acerifolia and its effect on plant morphology

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