Cytogenetics of in vitro cultured somatic cells and regenerated plants of barley (Hordeum vulgare L.)

Gaponenko, A. K.; Petrova, T. F.; Iskakov, A. R.; Sozinov, A. A.

doi:10.1007/bf00258052

Cited by 41 publications

(20 citation statements)

References 9 publications

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“…Similar results have been obtained by other authors (Ziauddin & Kasha, 1990;Hang & Bregitzer, 1993). However, Singh (1986) and Gaponenko et al (1988) observed that diploid cells predominated in morphogenic calli obtained from immature barley embryo while polyploid and aneuploid cells increased significantly or even predominated when calli became non-morphogenic. A correlation between chromosomal variability and morphogenic capability in barley embryogenic suspensions (Huang et al, 1993) has also been indicated.…”

Section: Discussionsupporting

confidence: 79%

“…Since one of the cultivars, Golden Promise, and the explant are commons in this latter work and in our work, the only variable to attribute the observed differences is the concentration of 2,4-D, 3 mg/l vs. 2 mg/1. Gaponenko et al (1988) carried out cytogenetic studies with morphogenic cultures for one year. A major part of meristematic cells in morphogenic calli were diploid at first.…”

Section: Discussionmentioning

confidence: 99%

“…In barley calli, karyotypic instability as a consequence of in vitro tissue culture has been repeatedly reported (Orton, 1980;Ruiz & V~izquez, 1981Ruiz & V~izquez, , 1982Singh 1986;Gaponenko et al, 1988;Mohanty, 1990;Ziauddin & Kasha, 1990;Hang & Bregitzer, 1993). In this species, in vitro plant regeneration is achieved via somatic embryogenesis.…”

Section: Introductionmentioning

confidence: 99%

“…The morphogenic potential of calli has been related to the lack of numerical and structural changes in the chromosomes (Singh, 1986;Gaponenko et al, 1988). Although explant and genotype may affect the karyotypic stability of the cultures, karyotypic differences have been mainly studied in cultures obtained from immature embryos (Singh, 1986;Karp et al, 1987;Gaponenko et al 1988;Ziauddin & Kasha, 1990;Hang & Bregitzer, 1993), but little is known about the karyotypic changes due to the nature of the explant within a given genotype (Mohanty, 1990).…”

Section: Introductionmentioning

confidence: 99%

“…Although explant and genotype may affect the karyotypic stability of the cultures, karyotypic differences have been mainly studied in cultures obtained from immature embryos (Singh, 1986;Karp et al, 1987;Gaponenko et al 1988;Ziauddin & Kasha, 1990;Hang & Bregitzer, 1993), but little is known about the karyotypic changes due to the nature of the explant within a given genotype (Mohanty, 1990). Likewise, to date, no work has compared the cytogenetical response of different explants using several cultivars, and only a few reports describe callus chromosome variation and its influence on plant regeneration (Gaponenko et al, 1988;Ziauddin & Kasha, 1990).…”

Section: Introductionmentioning

confidence: 99%

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Cytogenetic variation in somatic tissue cultures and regenerated plants of barley (Hordeum vulgare L.)

1996

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Chromosome number of morphogenic and non-morphogenic calli and regenerated plants of barley were determined. Cultures were obtained from two kinds of explants, immature embryos and seedling leaves from three cultivars, Ingrid, Dissa and Golden Promise. Callus chromosome analyses were carried out during a 12 month period in a medium containing 2 mg/1 of 2,4-D. Diploid cells were predominant in all cases; although in leaf-derived cultures tetraploid cells (2n --4x --28) showed a tendency to increase as time in culture increased and after more than six months in culture, diploid cells decreased to percentages of almost 70%. Aneuploid cells were generally infrequent in all cases. The source of explant has been more important than the genotype (cultivar) and the type of callus (morphogenic vs. non-morphogenic) in the chromosomal stability of cultures as time increases. From short term cultures, only 1.85% of the regenerated plants were tetraploid, the remaining were diploids. The ability of morphogenic calli to regenerate plants decreased before any significant reduction of diploid cells were observed.

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Section: Discussionsupporting

confidence: 79%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Cytogenetic variation in somatic tissue cultures and regenerated plants of barley (Hordeum vulgare L.)

1996

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Barley

Ganeshan

Chibbar

Dahleen

et al. 2008

Compendium of Transgenic Crop Plants

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The value of barley ( Hordeum vulgare L.) has steadily gained importance over the years. The economic and consumer importance of barley is constantly being re‐evaluated as new market niches and end‐uses become evident. Improvement strategies have generally employed conventional breeding methods, including mutation‐breeding approaches. However newer technology‐driven improvement strategies offer promising new perspectives and include genetic transformation. Barley was generally recalcitrant to tissue culture and transformation, but is now fairly amenable to genetic transformation by particle bombardment as well as by Agrobacterium . Target traits for modification have encompassed malting, nutritional and disease‐resistance attributes in barley. However, with the emerging trends in high‐throughput genomic sequencing and the need for functional validation of cloned genes, genetic transformation of barley has become an important tool and use of approaches such as transposable elements mediated transformation offers the possibility of producing large numbers of transgenic barley plants. Approaches based on the RNAi technology are also likely to advance understanding of the functions of cloned genes in barley. In this chapter, there is a wide‐ranging discussion on barley genetic transformation, prospects, challenges and risk assessment and environmental concerns.

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