Cytogenetics of Neuroblastoma : A Study Using Fine Needle Aspiration Cultures

Udayakumar, Achandira M.; Sundareshan, T.S.; Goud, Tadakal Mallana; Biswas, S.; Devi, Muniyappa Gayathri; Kumari, Babita; Appaji, L.

doi:10.1016/s0003-3995(01)01082-6

Cited by 7 publications

(2 citation statements)

References 21 publications

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“…39 NB cells are negative for CD99, cytokeratins, desmin, myogenin, smooth muscle actin, muscle-specific actin, CD34, S-100 protein, and CD45. 4,7,40 Furthermore, NB usually shows a deletion or rearrangement of material of chromosome 1p and amplification of N-myc, 41 and material from FNA can be used for these studies. 42 RMS cells are small, round, lymphocyte-like, and have a finely granular chromatin.…”

Section: Discussionmentioning

confidence: 99%

Fine‐needle aspiration of adult small‐round‐cell tumors studied with flow cytometry

Leon¹,

Hou²,

Galindo³

et al. 2004

Diagnostic Cytopathology

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Immunophenotypic study is critical for the diagnosis of adult small-round-cell tumors (SRCTs). We describe three patients with Ewing's sarcoma/primitive neuroectodermal tumor (ES/PNET) and one patient with neuroblastoma in which flow cytometry immunophenotyping (FCI) on the fine-needle aspirate (FNA) and bone marrow aspirate (BMA) demonstrated an abnormal population of cells that were CD45(-) and CD16/CD56(+). Four patients with mean age of 30 years, three male and one female, clinically suspicious for a lymphoma or SRCT are described. FNA, BMA, and biopsy specimens were obtained for routine cytologic and histologic evaluation. Fresh tissue was studied by FCI. In all cases, the cytology smears showed small cells with round nuclei, slightly irregular nuclear membranes, fine chromatin, and scant cytoplasm. FCI showed CD16/56(+) and CD45(-) neoplastic cells in all cases. In one case, 76% of these cells were CD99(+). The diagnoses of ES/PNET were confirmed by immunohistochemical, ultrastructural, and cytogenetic studies. ES/PNET in FNA and BMA can be efficiently and rapidly diagnosed by combining cytologic examination with FCI using a panel including CD45, CD16/56, and CD99.

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Section: Discussionmentioning

confidence: 99%

Fine‐needle aspiration of adult small‐round‐cell tumors studied with flow cytometry

Leon¹,

Hou²,

Galindo³

et al. 2004

Diagnostic Cytopathology

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“…Only single reports have analyzed the value of cytologic aspirates as cellular material used for genomic techniques. [3][4][5][6][7][8][9][10][11][12] Cytological diagnosis in pediatric tumors has been used at the Institut Curie since 1984. In our previous report of cases recruited between 1987 and 1999, 13 147 patients were diagnosed with neuroblastic tumors using cytology.…”

Section: Introductionmentioning

confidence: 99%

Diagnostic and prognostic information obtained on fine‐needle aspirates of primary neuroblastic tumors

Klijanienko

Couturier

Brisse

et al. 2011

Cancer Cytopathology

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BACKGROUND:The use of cytological material for diagnosis and prognosis in patients with neuroblastic tumors is poorly described in the literature. METHODS: A total of 129 patients with primary neuroblastic tumors underwent sampling with fine-needle aspiration for diagnosis and the evaluation of prognostic parameters. Of these, 125 (97%) were neuroblastomas or ganglioneuroblastomas and 4 (3%) were ganglioneuromas. Cellularity of the smears was assessed, as well as the percentage of neuroblasts versus stroma, maturation (differentiating/mature vs immature cells), the mitosis-karyorrhexis index (MKI), and the number of Homer Wright rosettes. The cytology samples were also analyzed for MYCN amplification, flow cytometric DNA index, and array comparative genomic hybridization. RESULTS: MYCN was found to be amplified in 35 (27%) cases. Strong correlations with overall survival were found for MYCN amplification in localized stages including IVs (P < .001), the percentage of neuroblasts versus stroma (P < .001), maturation (P ¼ .002), MKI (P < .001), and DNA index (P < .03). CONCLUSIONS: Poorly differentiated tumors with few stromal components and low numbers of differentiating/mature cells were found to be MYCN amplified. The authors propose a ''cytology prognostic score'' in which neuroblastomas comprised of predominantly neuroblastic elements with no differentiating cells and an MKI >; 2% are classified as high-risk tumors, regardless of their MYCN amplification status. The use of this proposed score would ensure accurate and optimal diagnostic and prognostic classifications of neuroblastic tumors in cases in which the histological biopsy is absent or inadequate for analysis. Cancer

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