Cytogenomic characteristics of murine breast cancer cell line JC

Azawi, Shaymaa; Rinčić, Martina; Liehr, Thomas

doi:10.1186/s13039-020-00524-z

Cited by 3 publications

(4 citation statements)

References 35 publications

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“…As a numerically variant karyotype with ~95 chromosomes was already reported in 1982 3 , it can be concluded that the MTLC-1 cell line remained relatively stable during the last 40 years, as also reported for other murine cell lines before. 4,[12][13][14][15][16][17][18][19][20][21][22][23][24] Like most of these cell lines, MTLC-1 also showed several dicentric chromosomes normally considered to be instable; also, MTLC-1 is one of the few cell lines with a stable neocentric chromosome. 19,22 By the molecular cytogenetic characterization scheme applied here, a detailed characterization of numerical and structural changes of MTLC-1 was performed, which enabled the determination of ploidy-grade, individual chromosome numbers and involved rearrangements.…”

Section: Discussionmentioning

confidence: 96%

First cytogenomic characterization of murine testis tumor cell line MLTC-1

Azawi

Kankel

Liehr

et al. 2022

Mol. exp. biol. med.

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The cell line MLTC-1 was established in 1982 as a transplantable Leydig cell tumor from a C57BL/6 mouse. The cell line has already been applied in >100 studies: still, the only information about its genetic content is given in the first description: MLTC-1 initially had a polyploid karyotype. Here, a molecular karyotyping and multicolor banding-based molecular cytogenetic study was done to provide the first chromosomal/ (molecular) cytogenetic characterization of MLTC-1. A hexaploid karyotype with 72 to 105 chromosomes was hereby characterized. Besides polyploidy, unbalanced two- and three-way translocations, dicentrics and one neocentric derivative were identified. Also, no Y-chromosome could be detected in this clearly male derived cell line. Overall, a completely unbalanced genome is present in MLTC-1 with ~20 regions being subject to copy number losses or gains. After translating these imbalances into the human genome in a standardized way, a 40% match of imbalances with human Leydig cell tumors was evident; however, about the same rate of concordance was detectable for human spermatocytic seminomas and non-seminomas as well as testicular germ cell tumor. Accordingly, MLTC-1 is better suited as an advanced testicular germ cell tumor model in general, rather than specifically for human Leydig cell tumors.

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Section: Discussionmentioning

confidence: 96%

First cytogenomic characterization of murine testis tumor cell line MLTC-1

Azawi

Kankel

Liehr

et al. 2022

Mol. exp. biol. med.

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“…The first cytogenomic characterization of the tumor cell line I-10 revealed a slightly hyperdiploid karyotype of 43 chromosomes, with relatively few imbalances and breakpoints for a cell line that is in cell culture for around 55 years. Interestingly, I-10 is a tumor cell line with dicentric chromosomes, which are normally considered to be unstable [5,[13][14][15][16][17][18][19][20][21][22][23][24][25]. It is one of the few cell lines with a stable neocentric chromosome [20,21].…”

Section: Discussionmentioning

confidence: 99%

“…Here, a comparison of translated I-10-specific imbalances was performed, similar to that performed for other murine cell lines [5,[13][14][15][16][17][18][19][20][21][22][23][24][25]. Sequencing analyses might also be fruitful in the future, as subtype-specific differences in somatic mutations were found in testicular germ cell tumors [31,32].…”

Section: Discussionmentioning

confidence: 99%

First Cytogenomic Characterization of the Murine Testicular Tumor Cell Line I-10

Azawi¹,

Barf²,

Liehr³

et al. 2022

OBM Genet

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After being established in 1967, the commercially available murine Leydig cell tumor line I-10 has been used in almost 50 published studies. I-10 has not been characterized, either at the chromosomal/ cytogenetic level or the genetic level, similar to many other murine tumor cell lines. In this study, we performed molecular karyotyping and multicolor banding-based molecular cytogenetics. A slightly hyperdiploid karyotype with 43 chromosomes was described. The main aberrations comprised several unbalanced translocations and three unusual rearrangements (two dicentric derivatives and one neocentric derivative). Nine regions showed copy number gains, and only five small chromosomal parts showed loss of copy numbers. A standardized translation of these imbalances in the human genome was performed, which showed a 63% overlap of the detected imbalances with testicular germ cell tumors, a 53% concordance with human spermatocytic seminomas and non-seminomas, and only a 36% overlap (approx.) of large copy number gains and losses were similar to the corresponding human Leydig cell tumors. However, no Y-chromosome was detected in this male-derived cell line. Overall, the I-10 cell line was found to be a testicular germ cell tumor model and cannot be treated as a model that is specific to human Leydig cell tumors. At best, it might be suited as a model for an early onset of Leydig cell tumors.

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“…JC cells, a murine primary breast cancer cell line characterized as basal-like or luminal B type [ 66 ], was purchased from Bioresource Collection and Research Center (Hsinchu, Taiwan) and cultured in the RPMI-1640 Medium (Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 1 mM sodium pyruvate and 4.5 g/L glucose. 4T1 cells, a murine triple-negative breast cancer cell line, was provided from American-Type Culture Collection (Manassas, VA) and maintained in RPMI-1640 Medium.…”

Section: Methodsmentioning

confidence: 99%

Diltiazem inhibits breast cancer metastasis via mediating growth differentiation factor 15 and epithelial-mesenchymal transition

Chen

et al. 2022

Oncogenesis

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Migration and metastasis commonly happen to triple-negative breast cancer (TNBC) patients with advanced diseases. In many studies, it has been suggested that epithelial-mesenchymal transition (EMT) is one of the key mechanisms triggering cancer metastasis. Accumulating evidence has proven that calcium channel blockers mediate cell motility. Therefore, we attempt to investigate the effects of diltiazem, which has been selected from several FDA-approved clinical calcium channel blockers, on EMT in TNBC. By using both mouse and human TNBC cell lines, we found that diltiazem decreases colony formation and cell migration in breast cancer cells. The expression of epithelial markers such as E-cadherin and ZO-1 were increased dose-dependently by diltiazem, while mesenchymal markers such as Snail and Twist were decreased. In addition, we found that the expression of growth differentiation factor-15 (GDF-15) was also increased by diltiazem. Administering recombinant GDF-15 also reverses EMT, inhibits colony formation and migration in breast cancer cells. Moreover, treatment with diltiazem in tumor-bearing mice also decreases cancer metastasis and nodule formation, with more GDF-15 expression in diltiazem-treated mice than saline-treated mice, respectively. These findings suggest that diltiazem regulates EMT and cell motility through elevating GDF-15 expression in breast cancers in vitro and in vivo.

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Cytogenomic characteristics of murine breast cancer cell line JC

Cited by 3 publications

References 35 publications

First cytogenomic characterization of murine testis tumor cell line MLTC-1

First cytogenomic characterization of murine testis tumor cell line MLTC-1

First Cytogenomic Characterization of the Murine Testicular Tumor Cell Line I-10

Diltiazem inhibits breast cancer metastasis via mediating growth differentiation factor 15 and epithelial-mesenchymal transition

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