Cytokine-induced release of ceramide-enriched exosomes as a mediator of cell death signaling in an oligodendroglioma cell line

Podbielska, Maria; Szulc, Zdzisław M.; Kurowska, Ewa; Hogan, Edward L.; Bielawski, Jacek; Bielawska, Alicja; Bhat, Narayan R.

doi:10.1194/jlr.m070664

Cited by 64 publications

(53 citation statements)

References 54 publications

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“…A subsequent study confirmed that Cer-enriched exosomes mediate cytotoxic effects in recipient cells. Specifically, exosomes released from HOG cells treated with a combination of inflammatory cytokines were found to be Cer-enriched and to induce cell death in fresh (naïve) HOG cells (108). Interestingly, Cer levels did not increase much in donor HOG cells under cytokine treatment, suggesting that Cer is immediately exported out of the cells by exosomes after generation.…”

Section: Sl-mediated Ev Action On Recipient Cellsmentioning

confidence: 95%

“…In particular, this study showed that SM is more represented in exosomes secreted across apical compared with the basolateral plasma membrane (107). iii) SL profiling of human oligodendroglioma (HOG) cell-derived exosomes, revealing differences in the SL composition, in particular of C16-, C24-, and C24:1-Cer species; C16-, C24-, and C24:1-dihydroCer species; and C16-, C24-, and C24:1-SM species, between exosomes released constitutively or under stimulation with inflammatory cytokines (108). iv) The work by Carayon et al (109) showing that the exosomal content of Cer versus SM is significantly increased during reticulocyte maturation.…”

Section: Comparative Analysis Of Sl Composition In Exosomes/ Mvs and mentioning

confidence: 99%

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Role of sphingolipids in the biogenesis and biological activity of extracellular vesicles

Verderio

Gabrielli

Giussani

2018

Journal of Lipid Research

182

136

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Extracellular vesicles (EVs) are membrane vesicles released by both eukaryotic and prokaryotic cells; they not only serve physiological functions, such as disposal of cellular components, but also play pathophysiologic roles in inflammatory and degenerative diseases. Common molecular mechanisms for EV biogenesis are evident in different cell biological contexts across eukaryotic phyla, and inhibition of this biogenesis may provide an avenue for therapeutic research. The involvement of sphingolipids (SLs) and their enzymes on EV biogenesis and release has not received much attention in current research. Here, we review how SLs participate in EV biogenesis by shaping membrane curvature and how they contribute to EV action in target cells. First, we describe how acid and neutral SMases, by generating the constitutive SL, ceramide, facilitate biogenesis of EVs at the plasma membrane and inside the endocytic compartment. We then discuss the involvement of other SLs, such as sphingosine-1-phosphate and galactosyl-sphingosine, in EV formation and cargo sorting. Last, we look ahead at some biological effects of EVs mediated by changes in SL levels in recipient cells.

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Section: Sl-mediated Ev Action On Recipient Cellsmentioning

confidence: 95%

Section: Comparative Analysis Of Sl Composition In Exosomes/ Mvs and mentioning

confidence: 99%

Role of sphingolipids in the biogenesis and biological activity of extracellular vesicles

Verderio

Gabrielli

Giussani

2018

Journal of Lipid Research

182

136

View full text Add to dashboard Cite

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“…Moreover, knockdown of Rab27a/b in an astrocyte-derived glioma cell line blocked glioma growth in vivo in mouse xenografts 59 . A likely cause of these different effects is that EV cargo is well known to differ based on the cell of origin 60 . In high-grade GBM tumors secrete EVs that contain many growth factors (e.g.…”

Section: Discussionmentioning

confidence: 99%

SMPD3suppresses IDH mutant tumor growth via dual autocrine-paracrine roles

Balakrishnan

Adnani

Chinchalongporn

et al. 2020

Preprint

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Oligodendrogliomas are lower-grade, slow-growing gliomas that are ultimately fatal. Although driver mutations are known, the mechanisms underlying their signature slow growth rates are poorly understood. We found evidence for intra-tumoral interactions between neoplastic and nonneoplastic cells in oligodendroglioma tissues. To further study these cell interactions, we used two patient-derived oligodendroglioma cell lines of lower and higher aggressivity. Both oligodendroglioma cell lines released extracellular vesicles that had cytotoxic effects on nonneoplastic and neoplastic cells, but each had distinct vesicular proteomes. Consistent with extracellular vesicles mediating growth inhibitory effects in oligodendrogliomas, higher expression levels of several extracellular vesicle biogenesis genes (SMPD3,TSG101, STAM1) correlates with longer survival in oligodendroglioma patients. Furthermore, SMPD3 overexpression slows oligodendroglioma cell growth in culture. Conversely, SMPD3 knockdown enhances oligodendroglioma proliferation in vitro, in murine xenografts, and in human cerebral organoid co-cultures. Oligodendroglioma-derived extracellular vesicles thus mediate tumor cell microenvironmental interactions that contribute to low aggressivity.

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“…Studies show that sphingolipids are important for intracellular signaling, particularly in promoting cell differentiation, proliferation and apoptosis [D'Auria and Bongarzone, 2016;Podbielska et al, 2016;Russo et al, 2016;Ueda, 2017]. In short, higher concentrations of sphingolipids may provide a "survival" or protective signaling to epithelia under conditions of oxidative stress; ceramide, the backbone of sphingolipids, has been shown to promote differentiation, proliferation, and apoptosis [Podbielska et al, 2016]. The notion that exosomes can transport protective messages under harsh conditions has been shown, specifically others have shown that exosomes generated in response to H 2 O 2 communicate protective messages in mice mast cell (MC/9) [Eldh et al, 2010].…”

Section: Discussionmentioning

confidence: 99%

Hydrogen Peroxide Stimulates Exosomal Cathepsin B Regulation of the Receptor for Advanced Glycation End‐Products (RAGE)

Downs

Dang

Johnson

et al. 2017

J of Cellular Biochemistry

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Exosomes are nano-sized vesicles that are secreted into the extracellular environment. These vesicles contain various biological effector molecules that can regulate intracellular signaling pathways in recipient cells. The aim of this study was to examine a correlation between exosomal cathepsin B activity and the receptor for advanced glycation end-products (RAGE). Type 1 alveolar epithelial (R3/1) cells were treated with or without hydrogen peroxide and exosomes isolated from the cell conditioned media were characterized by NanoSight analysis. Lipidomic and proteomic analysis showed exosomes released from R3/1 cells exposed to oxidative stress induced by hydrogen peroxide or vehicle differ in their lipid and protein content, respectively. Cathepsin B activity was detected in exosomes isolated from hydrogen peroxide treated cells. The mRNA and protein expression of RAGE increased in cultured R3/1 cells treated with exosomes containing active cathepsin B while depletion of exosomal cathepsin B attenuated RAGE mRNA and protein expression. These results suggest exosomal cathepsin B regulates RAGE in type 1 alveolar cells under conditions of oxidative stress. J. Cell. Biochem. 119: 599-606, 2018. © 2017 Wiley Periodicals, Inc.

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Cytokine-induced release of ceramide-enriched exosomes as a mediator of cell death signaling in an oligodendroglioma cell line

Cited by 64 publications

References 54 publications

Role of sphingolipids in the biogenesis and biological activity of extracellular vesicles

Role of sphingolipids in the biogenesis and biological activity of extracellular vesicles

SMPD3suppresses IDH mutant tumor growth via dual autocrine-paracrine roles

Hydrogen Peroxide Stimulates Exosomal Cathepsin B Regulation of the Receptor for Advanced Glycation End‐Products (RAGE)

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