Cytokine Production Induced by Low-Molecular-Weight Chemicals as a Function of the Stimulation Index in a Modified Local Lymph Node Assay: An Approach to Discriminate Contact Sensitizers from Respiratory Sensitizers

Och, François M.M van; Loveren, Henk Van; Jong, Wim H. de; Vandebriel, Rob J.

doi:10.1006/taap.2002.9473

Cited by 69 publications

(12 citation statements)

References 21 publications

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“…The observation that topical exposure of BALB/c strain mice to different classes of chemical allergen stimulates divergent immune responses at the level of cytokine expression has been confirmed by other investigators (Vandebriel et al, 2000;Hayashi et al, 2001;Manetz et al, 2001;Plitnick et al, 2002;Van Och et al, 2002). Protocol variations including assessment of cytokine production at the level of mRNA, rather than secreted protein product, measured by reverse transcription polymerase chain reaction (RT-PCR) or by ribonuclease protection assay (RPA), using freshly isolated cells or following culture…”

Section: Cytokine Secretion Profiles Provoked By Topical Exposure Of mentioning

confidence: 85%

Methods for the identiﬁcation of chemical respiratory allergens in rodents: comparisons of cytokine proﬁling with induced changes in serum IgE

Dearman

Skinner

Humphreys

et al. 2003

J of Applied Toxicology

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No validated or widely recognized test methods are currently available for the prospective identification of chemicals with the potential to cause respiratory allergy. The cellular and molecular mechanisms that result in the induction of chemical sensitization of the respiratory tract are unclear, although there is evidence for the selective development of T helper 2 (Th2)-type responses and, in some cases, the production of IgE antibody. We have therefore examined the utility of cytokine profiling using BALB/c mice, together with the measurement of induced increases in the total serum concentration of IgE in the Brown Norway (BN) rat, as markers for the prospective identification of chemical respiratory allergens. Responses provoked by the reference respiratory allergen trimellitic anhydride (TMA) have been compared with those stimulated by the respiratory sensitizing diisocyanates toluene diisocyanate (TDI) and hexamethylene diisocyanate (HDI) and by the acid anhydride hexahydrophthalic anhydride (HHPA). Topical exposure of BN rats to TMA, TDI and HHPA each provoked marked immune activation (increases in lymph node cellularity and proliferation). However, only treatment with TMA stimulated vigorous increases in the total serum concentration of IgE. In contrast, exposure to HHPA, TDI or HDI failed to provoke significant changes in serum IgE concentration or induced only transient and relatively weak increases in serum IgE levels. In parallel experiments using BALB/c strain mice, however, topical application of all four chemical respiratory allergens provoked a marked Th2-type cytokine secretion profile in draining lymph node cells. These data suggest that the measurement of induced changes in serum IgE is not sufficiently sensitive for the robust identification of chemical respiratory allergens. Furthermore, irrespective of the reasons for variations in TMA-induced IgE production among BN rats, doubts remain regarding the utility of these animals for the characterization of immune responses to chemical allergens. Cytokine profiling using the BALB/c strain mouse apparently provides a more robust method for the hazard assessment of chemical respiratory allergens.

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Section: Cytokine Secretion Profiles Provoked By Topical Exposure Of mentioning

confidence: 85%

Methods for the identiﬁcation of chemical respiratory allergens in rodents: comparisons of cytokine proﬁling with induced changes in serum IgE

Dearman

Skinner

Humphreys

et al. 2003

J of Applied Toxicology

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show abstract

“…Many studies have tried to characterize the chemical sensitizers according to the cytokine profiles they provoke (Dearman et al, 1996a(Dearman et al, , b, 2003Dearman and Kimber, 1999;Manetz and Meade, 1999;Hayashi et al, 2001;Manetz et al, 2001;Plitnick et al, 2002;van Och et al, 2002;Vandebriel et al, 2003). Changes (i.e., increases) in cytokine levels are likely related to T-helper cell proliferation that plays an important role in the induction and elicitation of contact sensitivity.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of non-radioactive endpoints ofex vivolocal lymph node assay-BrdU to investigate select contact sensitizers

Ülker

Ateş

Atak

et al. 2012

Journal of Immunotoxicology

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“…Many studies have tried to characterize the chemical sensitizers according to the cytokine profiles they provoke (Dearman et al, 1996a(Dearman et al, ,b, 1999(Dearman et al, , 2003Manetz and Meade, 1999;Hayashi et al, 2001;Manetz et al, 2001;Plitnick et al, 2002;Vandebriel et al, 2000Vandebriel et al, , 2003Van Och et al, 2002). The regulation of contact sensitivity responses by T H 1 and T H 2 cytokines has been studied intensively.…”

Section: Discussionmentioning

confidence: 99%

“…Evaluation of auricular lymph node cell lymphocyte proliferation and cytokine production as non-radioactive endpoints during murine contact allergy to the cytokine profiles (Dearman et al, 1994(Dearman et al, ,1996a(Dearman et al, ,b, 1999(Dearman et al, , 2003Manetz and Meade, 1999;Hayashi et al, 2001;Manetz et al, 2001;Vandebriel et al, 2000Vandebriel et al, , 2003Plitnick et al, 2002;Van Och et al, 2002, Ku et al, 2008. Cutaneous immune responses involving T-helper Type 1 (T H 1) and Type 2 (T H 2) cells, characterized by secretion of interferon (IFN)-γ and interleukin (IL)-2 and of IL-4 and IL-5, respectively, have been reported in ACD (Romagnani, 1991, Rowe andBunker, 1998 ;Ulrich et al, 2001).…”

Section: Research Articlementioning

confidence: 99%

Evaluation of auricular lymph node cell lymphocyte proliferation and cytokine production as non-radioactive endpoints during murine contact allergy

Ülker

Atak

Ateş

et al. 2011

Journal of Immunotoxicology

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Cytokine Production Induced by Low-Molecular-Weight Chemicals as a Function of the Stimulation Index in a Modified Local Lymph Node Assay: An Approach to Discriminate Contact Sensitizers from Respiratory Sensitizers

Cited by 69 publications

References 21 publications

Methods for the identiﬁcation of chemical respiratory allergens in rodents: comparisons of cytokine proﬁling with induced changes in serum IgE

Methods for the identiﬁcation of chemical respiratory allergens in rodents: comparisons of cytokine proﬁling with induced changes in serum IgE

Evaluation of non-radioactive endpoints ofex vivolocal lymph node assay-BrdU to investigate select contact sensitizers

Evaluation of auricular lymph node cell lymphocyte proliferation and cytokine production as non-radioactive endpoints during murine contact allergy

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