Cytokine profile of the blood in mice with normal and abnormal heart rhythm

Стенина, М. А.; Krivov, L. I.; Воеводин, Д. А.; Савчук, В. Й.; Ковальчук, Л. В.; Yarygin, V. N.

doi:10.1007/s10517-012-1608-9

Cited by 14 publications

(12 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In both cases, at 18 weeks postirradiation, the two weeks of KL 4 treatment resulted in essentially baseline levels of both cytokines, while untreated control and vehicle-treated irradiated mice both clearly showed increase over baseline. The wide variability in levels is consistent with other reported studies (44). For IL-2, it is notable that only the irradiated untreated control and vehicle-treated mice had levels outside of normal reference ranges (44).…”

Section: Resultssupporting

confidence: 93%

“…The wide variability in levels is consistent with other reported studies (44). For IL-2, it is notable that only the irradiated untreated control and vehicle-treated mice had levels outside of normal reference ranges (44). In fact, despite the variability in the data, reduction in KL 4 vs. untreated control for IL-2 was statistically significant (19 ± 3 pg/ml vs. 35 ± 5, P = 0.02) and KL 4 treatment returned IL-2 levels to the normal range.…”

Section: Resultssupporting

confidence: 93%

See 1 more Smart Citation

Radiation Mitigating Properties of Intranasally Administered KL₄Surfactant in a Murine Model of Radiation-Induced Lung Damage

Christofidou‐Solomidou

Pietrofesa

Arguiri

et al. 2017

Radiation Research

View full text Add to dashboard Cite

The threat of exposure to ionizing radiation from a nuclear reactor accident or deliberate terrorist actions is a significant public health concern. The lung is particularly susceptible to radiation-induced injury from external sources or inhalation of radioactive particles from radioactive fallout. Radiation-induced lung disease can manifest with an acute radiation pneumonitis and/or delayed effects leading to pulmonary fibrosis. As prior warning of radiation exposure is unlikely, medical countermeasures (MCMs) to mitigate radiation-induced lung disease that can be given in mass-casualty situations many hours or days postirradiation are needed to prevent both early and late lung damage. In this study, KL4 surfactant (lucinactant) was evaluated as a radiation mitigator in a well-characterized mouse model of targeted thoracic radiation exposure, for its effect on both early (several weeks) and late (18 weeks) lung damage. Here, 120 mg/kg total phospholipid of KL4 surfactant was administered twice daily intranasally, (enabling intrapulmonary inhalation of drug) to C57BL/6 mice 24 h after a single 13.5 Gy dose of thoracic irradiation (LD50 dose). Both early and chronic phase (2 and 4 weeks and 18 weeks postirradiation, respectively) assessments were performed. Mice were evaluated for evidence of reduced arterial blood oxygenation and early and chronic lung and systemic inflammation, lung fibrosis and oxidative stress. Analysis was done by performing lung function/respiration dynamics and measuring cellular protein content of bronchoalveolar lavage fluid (BALF), and levels of cytokines, 8-iso-prostaglandin F2α, hydroxyproline in lung and plasma, along with evaluating lung histology. The results of this study showed that intranasal delivery of KL4 surfactant was able to preserve lung function as evidenced by adequate arterial oxygen saturation and reduced lung inflammation and oxidative stress; total white count and absolute neutrophil count was decreased in BALF, as were plasma pro-inflammatory cytokine levels and biomarker of oxidative stress. KL4 surfactant is a promising MCM for mitigation of lung tissue damage after targeted, thoracic irradiation and has the potential to be developed as a broad-spectrum, multi-use MCM against chemical, biological, radiological or nuclear threat agents with potential to cause lung injury.

show abstract

Section: Resultssupporting

confidence: 93%

Section: Resultssupporting

confidence: 93%

Radiation Mitigating Properties of Intranasally Administered KL₄Surfactant in a Murine Model of Radiation-Induced Lung Damage

Christofidou‐Solomidou

Pietrofesa

Arguiri

et al. 2017

Radiation Research

View full text Add to dashboard Cite

show abstract

“…17 Moreover, there were no clear patterns indicating that these minor differences were entirely treatment dependent. In all cases, differences were not dose-dependent and cytokine responses in phage treated mice were at times lower than in control mice.…”

Section: Discussionmentioning

confidence: 98%

The impact of orally administered phages on host immune response and surrounding microbial communities

et al. 2016

View full text Add to dashboard Cite

Numerous studies have shown the efficacy of phage therapy in reducing foodborne pathogen carriage in food animals. Fewer studies have focused on host reactions, especially in terms of phage-mediated acute immune responses and effects on the gut microbiome. Here we administered E. coli O157:H7 phages in low (single dose of 10 5 PFU) or high (single dose of 10 7 PFU) quantities to mice. While there were time points at which cytokine levels in different treatment groups differed from one another, all cytokine levels remained within normal ranges for mice regardless of treatment. Similarly, the patterns of these differences were not dose related, indicating that phage treatment did not result in a strong acute immune response as measured here. In separate experiments, 3-week-old pigs received a diet containing an in-feed antibiotic or daily phage treatment. After two weeks, microbial DNA of ileal, cecal, and fecal contents was characterized using 16S rRNA sequencing. There were no statistical differences in performance among the different groups. Compared to control pigs (no antibiotic, no phage), antibiotic treatment significantly altered ileal microbiome composition (P < 0.05), with Bacilli being most affected (antibiotic treated: 22%; control: 76%; FDR D 0.0572). No significant differences were observed in cecal and fecal microbiome composition between antibiotic-treated and control pigs, and there were no differences in gut microbiome composition between phage treated and control pigs in any intestinal compartment. Significant abundance differences were observed at the OTU level, with OTUs belonging to genera such as Lactobacillus and Streptococcus being over-or underrepresented in either antibiotic or phage treated groups compared to control pigs. Determining whether these changes are deleterious to host, however, requires further study.

show abstract

“…Capoferri et al found in their studies that of the inflammatory markers, interleukin‐6 had the strongest correlation with relative bradycardia and other arrhythmias 7 . Interleukin‐6 is a significant inflammatory marker associated with the cytokine storm seen in COVID‐19 infection and has been reported to have direct impact on the sinoatrial node causing increased vagal tone and minimized heart rate variability ultimately leading to relative bradycardia 27‐29 . In addition to these hypotheses, as the disease course worsens arrhythmias are likely worsened by the hypoxia and multiorgan failure, resulting in electrolyte derangements and buildup of toxic metabolites 5,10 …”

Section: Mechanism Of Cardiac Involvementmentioning

confidence: 99%

COVID‐19 induced bradyarrhythmia and relative bradycardia: An overview

Douedi

Mararenko

Alshami

et al. 2021

Journal of Arrhythmia

View full text Add to dashboard Cite

Novel coronavirus 2019 (COVID‐19) has been the focus of the medical community since its emergence in December 2019 and has already infected more than 100 million patients globally. Primarily described to cause a respiratory illness, COVID‐19 has been found to affect almost every organ system. Bradycardia is a newly recognized ramification of COVID‐19 that still has unknown prognostic value. Studies have shown an increase in the incidence of arrhythmias, cardiomyopathies, myocarditis, acute coronary syndromes, and coagulopathies in infected patients as well as an increased risk of mortality in patients with preexisting cardiovascular disease. While the pathogenesis of bradycardia in COVID‐19 may be multifactorial, clinicians should be aware of the mechanism by which COVID‐19 affects the cardiovascular system and the medication side effects which are used in the treatment algorithm of this deadly virus. There has yet to be a comprehensive review analyzing bradyarrhythmia and relative bradycardia in COVID‐19 infected patients. We aim to provide a literature review including the epidemiology, pathogenesis, and management of COVID‐19 induced bradyarrhythmia.

show abstract

Cytokine profile of the blood in mice with normal and abnormal heart rhythm

Cited by 14 publications

References 8 publications

Radiation Mitigating Properties of Intranasally Administered KL₄Surfactant in a Murine Model of Radiation-Induced Lung Damage

Radiation Mitigating Properties of Intranasally Administered KL₄Surfactant in a Murine Model of Radiation-Induced Lung Damage

The impact of orally administered phages on host immune response and surrounding microbial communities

COVID‐19 induced bradyarrhythmia and relative bradycardia: An overview

Contact Info

Product

Resources

About

Cytokine profile of the blood in mice with normal and abnormal heart rhythm

Cited by 14 publications

References 8 publications

Radiation Mitigating Properties of Intranasally Administered KL4Surfactant in a Murine Model of Radiation-Induced Lung Damage

Radiation Mitigating Properties of Intranasally Administered KL4Surfactant in a Murine Model of Radiation-Induced Lung Damage

The impact of orally administered phages on host immune response and surrounding microbial communities

COVID‐19 induced bradyarrhythmia and relative bradycardia: An overview

Contact Info

Product

Resources

About

Radiation Mitigating Properties of Intranasally Administered KL₄Surfactant in a Murine Model of Radiation-Induced Lung Damage

Radiation Mitigating Properties of Intranasally Administered KL₄Surfactant in a Murine Model of Radiation-Induced Lung Damage