Cytokine Profiles in Tears Accompanying the Secondary Conjunctival Responses Induced by Nasal Allergy

Pelikán, Z

doi:10.3109/02713683.2013.836542

Cited by 7 publications

(7 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As most groups did not examine the amount of G-CSF in AR, little is known about its impact on AR. Pelikan [ 34 ] found elevated levels in tears of SAR patients after allergen provocation, providing evidence for an influence of G-CSF in this disease entity. But further research is needed to define its role in allergy.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Cytokine profiles in nasal fluid of patients with seasonal or persistent allergic rhinitis

König

Klemens

Eder

et al. 2015

Allergy Asthma Clin Immunol

View full text Add to dashboard Cite

BackgroundNew therapeutic approaches with biologic agents such as anti-cytokine antibodies are currently on trial for the treatment of asthma, rhinosinusitis or allergic diseases necessitating patient selection by biomarkers. Allergic rhinitis (AR), affecting about 20 % of the Canadian population, is an inflammatory disease characterised by a disequilibrium of T-lymphocytes and tissue eosinophilia. Aim of the present study was to describe distinct cytokine patterns in nasal secretion between seasonal and perennial AR (SAR/PAR), and healthy controls by comparing cytokines regulating T-cells or stimulating inflammatory cells, and chemokines.MethodsNasal secretions of 44 participants suffering from SAR, 45 participants with PAR and 48 healthy controls were gained using the cotton wool method, and analysed for IL-1β, IL-4, IL-5, IL-6, IL-10, IL-12, IL-13, IL-17, GM-CSF, G-CSF, IFN-γ, MCP-1, MIP-1α, MIP-1β, eotaxin, and RANTES by Bio-Plex Cytokine Assay as well as for ECP and tryptase by UniCAP-FEIA.ResultsParticipants with SAR or PAR presented elevated levels of tryptase, ECP, MCP-1, and MIP-1β, while values of GM-CSF, G-CSF, IL-1β, and IL-6 did not differ from the controls. Increased levels of IL-5, eotaxin, MIP-1α, and IL-17 and decreased levels of IFN-γ, IL-12 and IL-10 were found in SAR only. RANTES was elevated in SAR in comparison to PAR. Interestingly, we found reduced levels of IL-4 in PAR and of IL-13 in SAR.ConclusionsElevated levels of proinflammatory cytokines were seen in both disease entities. They were, however, more pronounced in SAR, indicating a higher degree of inflammation. This study suggests a downregulation of TH1 and Treg-lymphocytes and an upregulation of TH17 in SAR. Moreover, the results display a prominent role of eosinophils and mast cells in AR. The observed distinct cytokine profiles in nasal secretion may prove useful as a diagnostic tool helping to match patients to antibody therapies.

show abstract

Section: Discussionmentioning

confidence: 99%

“…Data on these two general markers of inflammation in nasal fluids are inconsistent [ 12 ]. Pelikan [ 34 ] did not find elevated levels of IL-6 in tears of allergic subjects. This is consistent with our results, but disagrees with an elevation of IL-1β and IL-6 found by others [ 9 ].…”

Section: Discussionmentioning

confidence: 99%

Cytokine profiles in nasal fluid of patients with seasonal or persistent allergic rhinitis

König

Klemens

Eder

et al. 2015

Allergy Asthma Clin Immunol

View full text Add to dashboard Cite

show abstract

“…IL-8 levels were higher in nasal biopsy specimens from patients with persistent AR vs. controls (114). Pelikan demonstrated high concentrations of IL-8 in tears from patients with AR after nasal provocation tests with allergen (115,116). In conclusion, IL-8 is a key player in the pathogenesis of asthma through its roles in neutrophil functions.…”

Section: Chemokines Associated With Function Of Th1 Lymphocytesmentioning

confidence: 81%

Relationship between chemokines and T lymphocytes in the context of respiratory allergies (Review)

et al. 2020

View full text Add to dashboard Cite

Allergic diseases have been classified in the last decades using various theories. The main classes of the newest classification in allergic respiratory diseases focus on the characterization of the endotype (which takes into account biomarkers related to determinant pathophysiological mechanisms) and of the phenotype (based on the description of the disease). Th2, Th1 and Th17 lymphocytes and the type of inflammatory response mediated by them represent the basis for Th2 and non-Th2 endotype classification. In addition, new lymphocytes were also used to characterize allergic diseases: Th9 lymphocytes, Th22 lymphocytes, T follicular helper cells (T FH) lymphocytes and invariant natural killer T (iNKT) lymphocytes. In the last decade, a growing body of evidence focused on chemokines, chemoattractant cytokines, which seems to have an important contribution to the pathogenesis of this pathology. This review presents the interactions between chemokines and Th lymphocytes in the context of Th2/non-Th2 endotype classification of respiratory allergies.

show abstract

“…The activation of mast cells in a Th2 microenvironment is a critical characteristic in allergic conjunctivitis inflammation [35]. At the ocular surface level, IL-10 is produced by conjunctival fibroblasts [36], by blood inflammatory cells such as basophils and eosinophils, and by local mast cells after an allergenic challenge [37,38]. Some authors have suggested a pathogenic role of IL-10 in ocular surface inflammatory diseases in which the Th2 profile is associated with ocular damage [39,40], and in a mice model of induced allergic conjunctivitis, IL-10 contributed to the development of the acute effector phase, augmenting the infiltration of eosinophils into the conjunctiva [41].…”

Section: Discussionmentioning

confidence: 99%

Low Expression of IL-10 in Circulating Bregs and Inverted IL-10/TNF-α Ratio in Tears of Patients with Perennial Allergic Conjunctivitis: A Preliminary Study

Salazar

Casanova-Méndez

Pacheco-Quito

et al. 2019

IJMS

View full text Add to dashboard Cite

Allergic conjunctivitis (AC) is one of the most common ophthalmological disorders seen in clinical practice. Growing evidence from recent years suggests that a subset of IL-10-expressing B cells is involved in inflammatory allergic diseases. In this study, we aimed to evaluate the potential involvement of blood Bregs cells in perennial allergic conjunctivitis (PAC), and interleukins (IL)-1β, IL-6, IL-8, IL-10, and IL-12, and tumor necrosis factor (TNF)-α, were measured in tear samples and compared with healthy controls (HC) using flow cytometry. Non-significant differences in CD19+IL-10+ cell frequency between PAC patients and healthy controls (HC) were observed. Nevertheless, when we analyzed the mean fluorescence intensity (MFI) of IL-10 on CD19+CD38Lo/Med/Hi-gated cells, we observed a significant decrease in MFI in all Bregs subsets in PAC patients. Additionally, tear cytokines showed 2.8 times lower levels of IL-10 than TNF-α in PAC patients when compared to HC. Our findings demonstrate an immunological dysregulation in patients with allergic conjunctivitis, characterized by the low expression of IL-10 in circulating CD19+CD38+ Bregs subsets and an inverted tear IL-10/TNF-α ratio, promoting a local pro-inflammatory microenvironment. These findings highlight the novel pathologic changes involved in ocular allergic diseases. Understanding systemic and local mechanisms will aid the design of immunomodulating therapeutics at different levels.

show abstract

Cytokine Profiles in Tears Accompanying the Secondary Conjunctival Responses Induced by Nasal Allergy

Cited by 7 publications

References 43 publications

Cytokine profiles in nasal fluid of patients with seasonal or persistent allergic rhinitis

Cytokine profiles in nasal fluid of patients with seasonal or persistent allergic rhinitis

Relationship between chemokines and T lymphocytes in the context of respiratory allergies (Review)

Low Expression of IL-10 in Circulating Bregs and Inverted IL-10/TNF-α Ratio in Tears of Patients with Perennial Allergic Conjunctivitis: A Preliminary Study

Contact Info

Product

Resources

About