Cytokine profiling in the sub-silicone oil fluid after vitrectomy surgeries for refractory retinal diseases

Kaneko, Hiroki; Takayama, Kei; Asami, Tetsu; Ito, Yasuki; Tsunekawa, Taku; Iwase, Takeshi; Funahashi, Yasuhito; Ueno, Shinji; Nonobe, Norie; Yasuda, Shunsuke; Suzumura, Ayana; Shimizu, Hideyuki; Kimoto, Reona; Hwang, Shiang-Jyi; Terasaki, Hiroko

doi:10.1038/s41598-017-03124-x

Cited by 27 publications

(23 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition, previous histopathological studies of eyes that have undergone ILM peeling have suggested that damage to Mü ller glial cells following ILM peeling may be responsible for the thinning of the temporal macula [21,39] . Another study reported that the fluid between the SO and the surface of the retina in SO-injected eyes has a higher expression of proinflammatory cytokines [40,41] . It is reasonable to speculate that the ILM plays a protective role against vitreous fluid containing higher levels of proinflammatory cytokines in eyes treated with SO endotamponade and that retinas without an ILM are directly exposed to the fluid, resulting in more severe damage.…”

Section: Discussionmentioning

confidence: 99%

Increased Retinal Thinning after Combination of Internal Limiting Membrane Peeling and Silicone Oil Endotamponade in Proliferative Diabetic Retinopathy

Kaneko

Matsuura²,

Takayama³

et al. 2017

Ophthalmologica

Self Cite

View full text Add to dashboard Cite

Background: The aim of this study was to examine the change in retinal thickness after vitrectomy with internal limiting membrane (ILM) peeling and/or silicone oil (SO) endotamponade in proliferative diabetic retinopathy (PDR). Methods: The actual amount and ratio of changes in the retinal thickness were calculated. Results: Compared to control eyes in the ILM peeling (-)/SO (-) group, the central, superior inner, and temporal inner retina in the ILM peeling (+)/SO (-) group, the central and superior inner retina in the ILM peeling (-)/SO (+) group, and the central, inferior inner, temporal inner, and nasal inner retina in the ILM peeling (+)/SO (+) group showed a significant reduction of the retinal thickness. The central, superior inner, and temporal inner retina in the ILM peeling (+)/SO (-) group, the central and superior inner retina in the ILM peeling (-)/SO (+) group, and the central, superior inner, inferior inner, and temporal inner retina in the ILM peeling (+)/SO (+) group showed a significantly increased reduction rate of the retinal thickness compared to the control group. Conclusions: Macular retinal thinning in PDR was observed after ILM peeling and SO endotamponade, and it was increased by the combination of these 2 factors.

show abstract

Section: Discussionmentioning

confidence: 99%

Increased Retinal Thinning after Combination of Internal Limiting Membrane Peeling and Silicone Oil Endotamponade in Proliferative Diabetic Retinopathy

Kaneko

Matsuura²,

Takayama³

et al. 2017

Ophthalmologica

Self Cite

View full text Add to dashboard Cite

show abstract

“…On the basis of the hypothesis that SOF has an important role in the pathogenesis of SORVL, we analyzed the inflammatory cytokine and electrolyte contents of SOF samples. We had already reported our finding that IL-6 and TNF-α levels in SOF samples were higher in eyes with revision surgery required–PVR than in those with simple PVR needing only SO evacuations at the time of the second surgeries 7. In contrast, FGF-2, IL-10, IL-12p40, IL-8, VEGF, and TGF-β1 were higher in the SOFs of eyes with revision surgery required–PDR than in those needing simple PDR 7.…”

Section: Discussionmentioning

confidence: 78%

“…We obtained written informed consent from all participating patients. We collected SOF samples as described 7. Briefly, before the beginning of the infusion, the edge of a 25-G blunt needle was placed above the surface of the posterior retina in a SO-filled eye using a RESIGHT surgical microscope (Zeiss, Oberkochen, Germany).…”

Section: Methodsmentioning

confidence: 99%

“…During the evacuation, a certain amount of fluid in the space between SO and the surface of the posterior retina can be found in the eye. We have dubbed this fluid “subsilicone oil fluid (SOF)” and have hypothesized that inflammatory cytokines in it have pivotal roles in SORVL induction 7. We have proposed a safe method to extract this SOF and have examined the levels of major inflammatory cytokines in the SOF from eyes with proliferative vitreoretinopathy (PVR), proliferative diabetic retinopathy (PDR), retinal detachment (RD), and macular hole (MH)-associated retinal detachment.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Biological Characteristics of Subsilicone Oil Fluid and Differences With Other Ocular Humors

Shimizu

Kaneko

Suzumura

et al. 2019

Trans. Vis. Sci. Tech.

Self Cite

View full text Add to dashboard Cite

Purpose: Subsilicone oil fluid (SOF) in eyes with silicone oil (SO) endotamponade possibly has a role in complications (e.g., vision loss); thus, we aimed to examine inflammatory cytokine and electrolyte levels and retinal glial cell viability in SOF. Methods:We measured major inflammatory cytokine levels and electrolytes in SOF and compared them with those in vitreous fluid (VF) and anterior chamber fluid (ACF). We analyzed the correlation between inflammatory cytokines and retinal thickness in SO-filled eyes. Further, we measured the MIO-M1 cell viability in medium with SOF and compared it with that containing VF.Results: We collected and examined 57 SOF, 22 ACF, and 21 VF samples from eyes with PVR, PDR, RD, and MH. Interleukin (IL)-8 and monocyte chemoattractant protein (MCP)-1 levels in SOF were significantly higher than those in ACF. There was no significant difference for all cytokines between SOF and VF. Retinal thickness changes during SO endotamponade were not correlated with the presence of any inflammatory cytokines. Levels of ferrous iron, but not of potassium, showed a significant decrease in SOF compared with VF. The WST-1 assay showed that SOFadded medium induced higher MIO-M1 cell viability than VF-added medium. Conclusions:We found no significant correlation between the change in the retinal thickness and cytokine levels, but SOF contains higher concentrations of cytokines and lower concentrations of ferrous iron and can be biologically distinguished from ACF and VF.Translational Relevance: Novel knowledge of inflammatory cytokine levels and electrolytes in SOF provides better understanding of pathology of SO-filled eyes.

show abstract

“…[78][79][80][81][82][83] IL-6 can also stimulate corneal epithelial cells and stromal fibroblasts (and macrophages) to produce profibrotic VEGF. 78 Like most inflammatory cytokines, IL-6 is present in subretinal fluid in high titers during retinal detachment and RRD repair, 84,85 and their presence is correlated with the subsequent, development of postoperative PVR, 9 as well as being elevated in the vitreous of patients with early PVR, 38,86 and correlating with PVR severity when found in sub silicone-oil fluid, 87 but, because subretinal and vitreous IL-6 levels significantly overlap between patients with uncomplicated retinal detachment and severe or future PVR, they have limited biomarker potential.…”

Section: Interleukin-6mentioning

confidence: 99%

Inflammatory and Fibrogenic Factors in Proliferative Vitreoretinopathy Development

Chaudhary

Scott²,

Wallace

et al. 2020

Trans. Vis. Sci. Tech.

View full text Add to dashboard Cite

Citation: Chaudhary R, Scott RAH, Wallace G, Berry M, Logan A, Blanch RJ. Inflammatory and fibrogenic factors in proliferative vitreoretinopathy development. Trans Vis Sci Tech. 2020;9(3):23, https://doi.org/10.1167/tvst.9.3.23Purpose: Proliferative vitreoretinopathy (PVR) occurs in 5%-10% of rhegmatogenous retinal detachment cases and is the principle cause for failure of retinal reattachment surgery. Although there are a number of surgical adjunctive agents available for preventing the development of PVR, all have limited efficacy. Discovering predictive molecular biomarkers to determine the probability of PVR development after retinal reattachment surgery will allow better patient stratification for more targeted drug evaluations.Methods: Narrative literature review. Results:We provide a summary of the inflammatory and fibrogenic factors found in ocular fluid samples during the development of retinal detachment and PVR and discuss their possible use as molecular PVR predictive biomarkers. Conclusions:Studies monitoring the levels of the above factors have found that few if any have predictive biomarker value, suggesting that widening the phenotype of potential factors and a combinatorial approach are required to determine predictive biomarkers for PVR.Translational Relevance: The identification of relevant biomarkers relies on an understanding of disease signaling pathways derived from basic science research. We discuss the extent to which those molecules identified as biomarkers and predictors of PVR relate to disease pathogenesis and could function as useful disease predictors.

show abstract

Cytokine profiling in the sub-silicone oil fluid after vitrectomy surgeries for refractory retinal diseases

Cited by 27 publications

References 33 publications

Increased Retinal Thinning after Combination of Internal Limiting Membrane Peeling and Silicone Oil Endotamponade in Proliferative Diabetic Retinopathy

Increased Retinal Thinning after Combination of Internal Limiting Membrane Peeling and Silicone Oil Endotamponade in Proliferative Diabetic Retinopathy

Biological Characteristics of Subsilicone Oil Fluid and Differences With Other Ocular Humors

Inflammatory and Fibrogenic Factors in Proliferative Vitreoretinopathy Development

Contact Info

Product

Resources

About