Cytokines and Their Roles in Pancreatic Islet β-Cell Destruction and Insulin-Dependent Diabetes Mellitus

Rabinovitch, Alex; Suarez-Pinzon, Wilma L.

doi:10.1016/s0006-2952(97)00492-9

Cited by 436 publications

(368 citation statements)

References 101 publications

Supporting

Mentioning

347

Contrasting

Unclassified

Order By: Relevance

“…However, TNF has opposing effects on T1DM. It is shown in an animal model that TNF can be, supported by both interleukin-1 and interferon-γ, toxic for β-cells [11]. Also, nonobese diabetic (NOD) mice that overexpressed TNF-α in their β-cells are predisposed to diabetes [12].…”

Section: Introductionmentioning

confidence: 99%

“…However, several other studies found an independent effect of TNF/LTA with T1DM [17][18][19][20]. TNF involvement in the destruction of pancreatic β-cells [10,11] and multiple inflammatory activities of TNF and LTA cytokines make them plausible targets for revealing molecular susceptibility to T1DM. The joint analysis of TNF and LTA genes may be more informative in defining the risk/protection to T1DM susceptibility [7,18].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Family-based analysis of tumor necrosis factor and lymphotoxin-α tag polymorphisms with type 1 diabetes in the population of South Croatia

et al. 2009

View full text Add to dashboard Cite

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Family-based analysis of tumor necrosis factor and lymphotoxin-α tag polymorphisms with type 1 diabetes in the population of South Croatia

et al. 2009

View full text Add to dashboard Cite

show abstract

“…They were present, however, at a very low level, with cycle threshold values between 32 and 33, while expression levels of Tnf-α and Ifn-γ were very close to the detection limit, with cycle threshold values at least another two decimal powers lower than for Il-1β. On the other hand, immune cells of lymphoid tissues from Psammomys obesus, namely pancreas-draining lymph nodes and spleen, expressed all three proinflammatory cytokines, as well as iNOS, at high levels, comparable to those typically found in infiltrated islets of type 1 diabetes animal models [21,22]. While the source of the high level expression of cytokines in type 1 diabetes infiltrated islets is clearly the infiltrating immune cells [16,23], it is likely that this low level of mRNA in P. obesus islets represents expression of the genes encoding cytokines in beta cells, like that also observed in human [13] and rat [24] pancreatic beta cells.…”

Section: Discussionmentioning

confidence: 85%

Beta cell death in hyperglycaemic Psammomys obesus is not cytokine-mediated

2006

View full text Add to dashboard Cite

Aims/hypothesis It has recently been proposed that IL-1β may be responsible for beta cell death in type 2 diabetes mellitus. Major support for this assumption was derived from experiments in the gerbil Psammomys obesus (sand rat), a model for nutritionally induced non-insulin-dependent type 2 diabetes. Using gerbil-specific primers for the analysis of gene expression, we investigated the validity of this hypothesis. Methods Gene expression was analysed by real-time RT-PCR of isolated and laser-microdissected islets and by in situ RT-PCR, both in beta cells and in immune cells, as well as in lymph nodes and spleen. Results We were unable to detect Il-1β and the IL-1β-inducible enzyme inducible nitric oxide synthase (iNos) by in situ RT-PCR, either in the pancreatic beta cells, or in the small number of non-activated immune cells of healthy and diabetic Psammomys obesus after 1 and 3 weeks on a highenergy diet. Very low levels of Il-1β and iNos mRNA were detectable in collagenase-isolated and laser-microdissected islets of normoglycaemic gerbils by real-time RT-PCR without any increase of these mRNAs in islets from diabetic animals. These results were confirmed by electron microscopy with immunogold staining for IL-1β and insulin. Conclusions/interpretation The diabetic syndrome induced in Psammomys obesus by high-energy diet is a classical type 2 diabetes model, which does not show any evidence of an involvement of the proinflammatory cytokine IL-1β or of activated immune cells in its pathogenesis. This is clearly at variance with the situation in type 1 diabetes.

show abstract

“…Islets pretreated with free L-glutamine or NALG for 24 h and then stressed with H2O2, ETA or cytokine mix 24 h Viability: L-glutamine ↑ NALG 1 Giovagnoli et al [31] , 2005…”

Section: Antioxidant Recruitment In Islet Transplantation Studiesmentioning

confidence: 99%

“…In a genetically susceptible person with an environmental trigger such as viruses and toxins, autoimmune destruction of β cells can occur causing type 1 diabetes usually in childhood and in young adults [1] . Type 2 diabetes usually results from dysfunction of β cells and peripheral insulin resistance.…”

Section: Introductionmentioning

confidence: 99%

Islet transplantation and antioxidant management: A comprehensive review

Monfared¹,

Larijani

Abdollahi

2009

WJG

View full text Add to dashboard Cite

Islet transplantation as a promising treatment for type 1 diabetes has received widespread attention. Oxidative stress plays an essential role in cell injury during islet isolation and transplantation procedures. Antioxidants have been used in various studies to improve islet transplantation procedures. The present study reviews the role of oxidative stress and the benefits of antioxidants in islet transplantation procedures. The bibliographical databases Pubmed and Scopus were searched up to November 2008.All relevant human and animal in-vivo and in-vitro studies, which investigated antioxidants on islets, were included. Almost all the tested antioxidants used in the in-vitro studies enhanced islet viability and insulin secretion. Better control of blood glucose after transplantation was the major outcome of antioxidant therapy in all in-vivo studies. The data also indicated that antioxidants improved islet transplantation procedures. Although there is still insufficient evidence to draw definitive conclusions about the efficacy of individual supplements, the benefits of antioxidants in islet isolation procedures cannot be ignored.

show abstract

Cytokines and Their Roles in Pancreatic Islet β-Cell Destruction and Insulin-Dependent Diabetes Mellitus

Cited by 436 publications

References 101 publications

Family-based analysis of tumor necrosis factor and lymphotoxin-α tag polymorphisms with type 1 diabetes in the population of South Croatia

Family-based analysis of tumor necrosis factor and lymphotoxin-α tag polymorphisms with type 1 diabetes in the population of South Croatia

Beta cell death in hyperglycaemic Psammomys obesus is not cytokine-mediated

Islet transplantation and antioxidant management: A comprehensive review

Contact Info

Product

Resources

About