Cytokines in childhood hemolytic uremic syndrome and thrombotic thrombocytopenic purpura

Karpman, Diana; Andreasson, Annika; Thysell, Hans; Kaplan, Bernard S.; Svanborg, Catharina

doi:10.1007/bf00868714

Cited by 122 publications

(102 citation statements)

References 20 publications

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“…5,7,[16][17][18] Previous studies on cytokines involved in TTP/HUS have suggested a role for TNF-␣ and IL-6. 22 In the present study, however, neither of these cytokines showed any significant differences between patients with and without TM (Figure 1b and c). Only IL-12 showed a significant increase between the aplastic phase and the recovery phase in the patients with TM when compared to those without TM (Figure 1a).…”

Section: Discussioncontrasting

confidence: 68%

“…23,[25][26][27][28] Other actions which have been reported include induction of vascular permeability factor, 25 release of other cytokines (such as TNF-␣, IL-8 and IL-2) by induction of natural killer cells and T cells, and aggravation of acute GVHD. 29 In summary, BMT was complicated by TM following exposure to stimuli such as severe GVHD, CMV infection, and high CsA concentrations when patients had become Various cytokines and ICAM-1 were measured five times: before conditioning, day 0 (before bone marrow infusion), during the aplastic phase (days 5-10), during rapid recovery of WBC (days [11][12][13][14][15][16][17][18][19][20], and during the stable phase after WBC recovery and discontinuation of G-CSF (days [21][22][23][24][25][26][27][28]. The only significant difference in cytokines between the patients with and without TM was a marked increase of interleukin-12 (IL-12) that occurred by the WBC recovery phase in the patients with TM (two-way layout analysis of variance; P Ͻ 0.05) (a).…”

Section: Discussionmentioning

confidence: 99%

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Thrombotic microangiopathy following allogeneic bone marrow transplantation

Takatsuka

Takemoto

Okamoto

et al. 1999

Bone Marrow Transplant

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Summary:Thrombotic microangiopathy is one of the complications of bone marrow transplantation and is related to other complications such as graft-versus-host disease, veno-occlusive disease, diffuse alveolar hemorrhage, and cytomegalovirus infection. Thrombotic microangiopathy occurred in three out of 12 patients who underwent allogeneic bone marrow transplantation over the past 1 year at our department. We compared the changes in cytokines and other molecules between patients with and without microangiopathy from before conditioning to the early post-transplantation period. All three patients with microangiopathy showed a significant increase of interleukin-12 at the time of leukocyte recovery after transplantation (two-way layout analysis of variance; P Ͻ 0.05), while none of the patients without microangiopathy showed an increase of interleukin-12. No significant differences were found between the two groups with respect to the other cytokines and molecules that were tested. These findings suggested that thrombotic microangiopathy might be predicted at an early stage after bone marrow transplantation by detecting an increase of interleukin-12 at the time of leukocyte recovery. The possibility that thrombotic microangiopathy is related to inflammation or autoimmunity was also suggested.

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Section: Discussioncontrasting

confidence: 68%

Section: Discussionmentioning

confidence: 99%

Thrombotic microangiopathy following allogeneic bone marrow transplantation

Takatsuka

Takemoto

Okamoto

et al. 1999

Bone Marrow Transplant

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“…Some kinds of circulating cytokines and the soluble receptors drastically increased with the onset of HUS, including TNF-a, FGF, IL-1b, IL-6, IL-8, IL-10, sTNFR1, sIL-2R, and sIL-4R [12,[17][18][19][20][21][22][23]. Thus, sIL-9R level of the patients with HUS should be shifted.…”

Section: Discussionmentioning

confidence: 99%

Age-Related Changes of Serum Soluble Interleukin 9 Receptor (sIL-9Rα) in Healthy Subjects

et al. 2012

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Most cytokine receptors including interleukin (IL)-9 have soluble counterparts in body fluids. We planned to investigate the pathophysiological significance of the serum soluble IL-9 receptor (sIL-9R) level. We determined the serum sIL-9Ra chain (sIL-9Ra) levels in 96 healthy Japanese individuals to establish a control value by means of specific human sIL-9Ra ELISA, followed by a preliminary application in a patient with diarrhea positive hemolytic uremic syndrome. Age was negatively correlated with the sIL-9Ra level (Spearman r = -0.241, n = 96, p = 0.0180). The serum sIL-9Ra level showed a progressive decline to the normal adult level by the age of 30. The serum sIL-9Ra level of the patient with HUS was markedly higher than those of the age-matched control from the onset of the disease. Because of the remarkable age-dependent variability of sIL-9Ra in healthy subjects, disease-related changes, as well as therapy-dependent alterations, should be considered with caution. Thus, it is recommended that when the serum sIL-9Ra levels of patients are evaluated, the values should be compared with those of age-matched controls. The established control value will be used to discriminate between normal and the pathological conditions in our future studies.Keywords Soluble interleukin 9 receptor a chain (sIL-9Ra) Á Hemolytic uremic syndrome (HUS) Á ELISA

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“…Elevations of TNF-a, IL-1, and IL-6 were described in patients with disseminated intravascular coagulation (DIC) as well as in TTP, but not in patients with red-cell fragmentation syndrome induced by mitomycin C [22]. In childhood TTP/hemolytic uremic syndrome, IL-6 and TNF-a were shown to be elevated [23]. In kidney/pancreas transplant patients who developed TTP following treatment with tacrolimus, IL-8, IL-10, and IL-12 were elevated during the acute phase in all three reported patients [24].…”

Section: Discussionmentioning

confidence: 99%

“…TGF-b1 upregulation has also been reported to be partially protective, suppressing microvascular endothelial cell damage via its anti-apoptotic properties [20]. Measurements of other cytokines including pro-inflammatory cytokines have produced variable results and have been limited to measurements at the disease onset and complete remission [21][22][23][24][25]. In this pilot study, we used a novel multiplexing bead array technique that allowed for simultaneous measurement of multiple cytokines in single serum samples.…”

Section: Introductionmentioning

confidence: 99%

Effect of plasma exchange on cytokines measured by multianalyte bead array in thrombotic thrombocytopenic purpura

2005

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The serum cytokine profile in thrombotic thrombocytopenic purpura (TTP) has not been extensively characterized. In this pilot study, a novel technique was utilized to evaluate multiple cytokines in patients with idiopathic TTP during a course of plasma exchange (PE). Single serum samples were obtained from five TTP patients before and after each PE. Random sera were obtained from nine healthy volunteers who served as controls. The samples were evaluated for 13 cytokines (IL-1B, IL-2 , IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12, IL-13, IFN-g , GM-CSF, and TNF-a) using the Luminex bead array (LINCOplex) multianalyte detection system that permits simultaneous detection of multiple cytokines from a single sample. Each patient received 4-6 PEs (Cobe Spectra) with 3.0-4.0 L of fresh frozen plasma as replacement fluid. Four of 5 patients received corticosteroids prior to and during PE. The control group did not receive steroids. Baseline values for IL-8 (182.9 vs. 6.5 pg/mL, P < 0.05) and TNF-a (11.4 vs. 0.9 pg/mL, P < 0.001) were significantly higher in TTP patients compared with controls. Other tested cytokines were not significantly different between the two groups. Comparison of cytokine values pre-and post-PE indicate a substantial decrease after each PE. However, cytokines rebounded to abnormal levels by the following day. There was no correlation between cytokines and serum LDH or platelet count. These findings suggest that certain cytokines, particularly IL-8 and TNF-a, are altered in TTP, and this may indicate a direct role in TTP pathogenesis, reflect ongoing tissue injury, or perhaps indicate an inadequate attempt to limit tissue injury.

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Cytokines in childhood hemolytic uremic syndrome and thrombotic thrombocytopenic purpura

Cited by 122 publications

References 20 publications

Thrombotic microangiopathy following allogeneic bone marrow transplantation

Thrombotic microangiopathy following allogeneic bone marrow transplantation

Age-Related Changes of Serum Soluble Interleukin 9 Receptor (sIL-9Rα) in Healthy Subjects

Effect of plasma exchange on cytokines measured by multianalyte bead array in thrombotic thrombocytopenic purpura

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