Cytokines locally produced by lymphocytes removed from the hypertrophic nasopharyngeal and palatine tonsils

Komorowska, A; Komorowski, Jan; Banasik, Mirosław; Lewkowicz, Przemysław; Tchórzewski, H

doi:10.1016/j.ijporl.2005.01.035

Cited by 48 publications

(38 citation statements)

References 31 publications

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“…T-cell derived IFN-g and IL-10 may be key regulators of the production of mucosal antibody to pneumococcal protein antigens in the nasopharynx and may play an important role in local protection against pneumococcal infection in children [26]. Komorowska [27]. Our results clearly suggest that in the adenoid tissue of children suffering from otitis media with effusion the prevailing T cell response was of the Th2 type due to the higher production of IL-5.…”

Section: Discussionmentioning

confidence: 64%

Production of cytokines by mononuclear cells of hypertrophic adenoids in children with otitis media with effusion

Żelazowska-Rutkowska¹,

Iłendo²,

Skotnicka³

et al. 2012

Folia Histochem Cytobiol.

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Hypertrophic adenoids with otitis media with effusion is a common infectious disease and present a serious otological problem in children. Cytokines, potent inflammatory mediators, play important role in the initiation of immunological response in otitis media. Adenoids excised due to hypertrophy with or without chronic otitis media with effusion were used to isolate mononuclear cells. Secretion of cytokines by non-stimulated and PHA-stimulated cells was determined by specific ELISAs. We found a significant increase in the production of IL-5 and TNF-a secreted by adenoidal cells of children with otitis media with effusion compared to group with hypertrophic adenoids. No differences were found in the secretion of IL-8, IL-6, and IL-10 between these two groups of patients. Our results suggest a difference between the immunological responses in the course of hypertrophic adenoids with otitis media as compared to hypertrophic adenoids.

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Section: Discussionmentioning

confidence: 64%

Production of cytokines by mononuclear cells of hypertrophic adenoids in children with otitis media with effusion

Żelazowska-Rutkowska¹,

Iłendo²,

Skotnicka³

et al. 2012

Folia Histochem Cytobiol.

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“…Published evidence would support a role for cysLT in T-cell lymphocyte proliferation and activation, 25,31 and furthermore, T-cell proliferation leads to proinflammatory cytokine production in adenotonsillar tissues. 32,33 Thus, future studies should explore the specific effects of cysLT 1 and 2 receptor antagonists on the proliferation of T-cell subsets in adenotonsillar cultures in children with OSA.…”

Section: Discussionmentioning

confidence: 99%

Leukotriene Pathways and In Vitro Adenotonsillar Cell Proliferation in Children With Obstructive Sleep Apnea

Dayyat

Serpero

Kheirandish‐Gozal

et al. 2009

Chest

109

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Introduction:The abundant expression of leukotrienes (LTs) and their receptors in adenotonsillar tissues of children with obstructive sleep apnea (OSA) suggest that LT antagonists could be useful in treating OSA. Methods: The effects of LTD4 and of LT receptor antagonists zileuton, montelukast, and BAY u9773 were examined on mixed cell cultures prepared from dissociated tonsils or adenoids harvested intraoperatively from children with polysomnographically diagnosed OSA. Proliferation was assessed by 3 [H]-thymidine incorporation, and inflammatory cytokine production (tumor necrosis factor [TNF]-␣, interleukin [IL]-6, IL-8, IL-10, and IL-12) was assessed in supernatants using enzyme-linked immunosorbent assay.Results: LTD4 elicited dose-dependent increases in adenotonsillar cell proliferation (p < 0.001; n ‫؍‬ 12). All LT antagonists exhibited dose-dependent reductions in adenotonsillar cellular proliferation rates, with montelukast more than BAY u9773 more than zileuton (n ‫؍‬ 14/group; p < 0.001). However, BAY u9773 showed partial agonist effects and increased cellular proliferation at higher concentrations (10 ؊4 mmol/L; p < 0.01; n ‫؍‬ 12). LTD4 effects were partially blocked by montelukast and BAY u9773 but not by zileuton. All three antagonists reduced TNF-␣, IL-6, and IL-12 concentrations, with selective changes in IL-8 and no effects on IL-10 levels. Conclusions: LT pathways mediate intrinsic proliferative and inflammatory signaling pathways in adenotonsillar tissues from children with OSA, and targeted pharmacologic disruption of these pathways may provide nonsurgical alternatives for prevention and treatment of this disease.

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“…Similarly, FLU has been found to have increased potency compared with BUD in inhibiting human T-cell migration and proliferation, and inhibiting CD4+ T-cell cytokine release, and in stimulating inflammatory cell apoptosis [30]. Considering the increased abundance of T-cells, particularly CD4+ T-cells, in the tonsillar tissues of children with OSA [31], and the important roles that these cell populations may have in local regulation of immune responses and proliferation [32][33][34], the use of CS as therapeutic tools seems a logical approach in an attempt to reduce T-cell proliferation and promote apoptosis, while reducing pro-inflammatory cytokine production [35][36][37].…”

Section: Discussionmentioning

confidence: 99%

Corticosteroids suppressin vitrotonsillar proliferation in children with obstructive sleep apnoea

Kheirandish‐Gozal¹,

Serpero²,

Dayyat³

et al. 2008

Eur Respir J

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Intranasal corticosteroids (CS) are potentially useful interventions for children with obstructive sleep apnoea (OSA), and may reduce lymphadenoid tissue size in the upper airway. The present authors hypothesised that CS would reduce cellular proliferation and the production of pro-inflammatory cytokines in a tonsil/adenoid mixed-cell culture system. Dissociated tonsils or adenoids harvested intra-operatively from children with polysomnographically diagnosed OSA were cultured in control medium (CO) or after stimulation with lipopolysaccharide and concanavalin A (STIM), and incubated with dexamethasone (DEX; 10 ; n554). DEX, FLU and BUD reduced cellular proliferation rates, and exhibited dose-dependent effects, with the potency being FLU.BUD.DEX (n525 per group). Conversely, CS increased cellular apoptosis (n520 per group). Furthermore, TNF-a, IL-8 and IL-6 concentrations in the supernatant were increased by STIM, and markedly reduced by all CS (n548 per group).Whole tissue cell cultures of adenoids and tonsils provide a useful approach for in vitro assessment of therapeutic efficacy of corticosteroids in the management of lymphadenoid hypertrophy that underlies obstructive sleep apnoea in children.

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Cytokines locally produced by lymphocytes removed from the hypertrophic nasopharyngeal and palatine tonsils

Cited by 48 publications

References 31 publications

Production of cytokines by mononuclear cells of hypertrophic adenoids in children with otitis media with effusion

Production of cytokines by mononuclear cells of hypertrophic adenoids in children with otitis media with effusion

Leukotriene Pathways and In Vitro Adenotonsillar Cell Proliferation in Children With Obstructive Sleep Apnea

Corticosteroids suppressin vitrotonsillar proliferation in children with obstructive sleep apnoea

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