Cytokinin dehydrogenase: a genetic target for yield improvement in wheat

Chen, Lei; Zhao, Jingjing; Song, Jiancheng; Jameson, Paula E.

doi:10.1111/pbi.13305

Cited by 113 publications

(151 citation statements)

References 127 publications

(217 reference statements)

Supporting

Mentioning

146

Contrasting

Order By: Relevance

“…None exhibits the same role in the GC and in the field. Zhang et al [ 35 ] reported that TaCKX6 , renamed by [ 20 ] as TaCKX2.2.1-3D , is an orthologue of OsCKX2 regulating rice yield [ 10 ]. The cultivar, which has an 18-bp deletion, showed decreased expression of this gene, which was associated with a greater TGW than in other haplotypes.…”

Section: Discussionmentioning

confidence: 99%

“…TaCKX2 underwent gene duplication [ 15 ] and the resultant genes were assigned as TaCKX2.1 and TaCKX2.2 based on the Ensembl Plants database [ 19 ] and phylogenetic analysis by Ogonowska et al [ 16 ]. The numbering of TaCKX GFMs was recently revised taking advantage of the most up-to-date databases (including IWGSC RefSeq v2.0), their homology with closely related monocot species including barley and diploid genome specificity [ 20 ]. Based on these data, there are 13 TaCKX GFMs basically numbered as TaCKX1 , TaCKX2.1 , TaCKX2.2.1 , TaCKX2.2.2 , TaCKX2.2.3 , TaCKX3 ( 6 ), TaCKX4 , TaCKX5 , TaCKX7 , TaCKX8 ( 11 ), TaCKX9 ( 10 ), TaCKX10 ( 9 ) and TaCKX11 ( 3 ), and 12 of them are allocated to each of the three chromosomes associated with the A, B and D genomes, giving the number of 35 homoeologous genes.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Different sets of TaCKX genes affect yield-related traits in wheat plants grown in a controlled environment and in field conditions

et al. 2020

View full text Add to dashboard Cite

Background TaCKX wheat gene family members (GFMs) encode the enzyme cytokinin oxidase/dehydrogenase (CKX), which irreversibly degrades cytokinins. The genes are important regulators of cytokinin content and take part in growth and development, with a major impact on yield-related traits. The goal of this research was to test whether these genes might be differentially expressed in the field compared to laboratory conditions and consequently differently affect plant development and yield. Results We compared expression and crosstalk of the TaCKX GFMs and TaNAC2-5A gene in modern varieties grown in a growth chamber (GC) and in the field and looked for differences in their impact on yield-related traits. The TaNAC2-5A gene was included in the research since it was expected to play an important role in co-regulation of these genes. The range of relative expression levels of TaCKX GFMs and TaNAC2-5A gene among tested cultivars was from 5 for TaCKX8 to more than 100 for TaCKX9 in the GC and from 6 for TaCKX8 to 275 for TaCKX10 in the field. The range was similar for four of them in the GC, but was much higher for seven others and TaNAC2-5A in the field. The TaCKX GFMs and TaNAC2-5A form co-expression groups, which differ depending on growth conditions. Consequently, the genes also differently regulate yield-related traits in the GC and in the field. TaNAC2-5A took part in negative regulation of tiller number and CKX activity in seedling roots only in controlled GC conditions. Grain number and grain yield were negatively regulated by TaCKX10 in the GC but positively by TaCKX8 and others in the field. Some of the genes, which were expressed in seedling roots, negatively influenced tiller number and positively regulated seedling root weight, CKX activity in the spikes, thousand grain weight (TGW) as well as formation of semi-empty spikes. Conclusions We have documented that: 1) natural variation in expression levels of tested genes in both environments is very high, indicating the possibility of selection of beneficial genotypes for breeding purposes, 2) to create a model of an ideotype for breeding, we need to take into consideration the natural environment.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Different sets of TaCKX genes affect yield-related traits in wheat plants grown in a controlled environment and in field conditions

et al. 2020

View full text Add to dashboard Cite

show abstract

“…The other important component of CK metabolism is the CKX enzyme, which accounts for the irreversible inactivation of CKs by cleaving the side chain from CK molecules [46]. Genome-wide association studies have recently shed more light on the potential function of CKX members in plant species, with 13 CKX GFMs in maize [71], 11 CKX GFMs in rice [66], 12 CKX GFMs in apple [67], CKX GFMs in wheat (Triticum aestivum) [72] and 23 CKX GFMs in rapeseed (Brassica napus) [73]. Increasing lines of evidence suggest that CKX GFMs play important roles in various plant physiological and developmental modifications under drought stress [74][75][76] (Table 1).…”

Section: Response Of Ck Metabolic Genes To Droughtmentioning

confidence: 99%

Role and Regulation of Cytokinins in Plant Response to Drought Stress

Hải

Chuong

et al. 2020

Plants

103

View full text Add to dashboard Cite

Cytokinins (CKs) are key phytohormones that not only regulate plant growth and development but also mediate plant tolerance to drought stress. Recent advances in genome-wide association studies coupled with in planta characterization have opened new avenues to investigate the drought-responsive expression of CK metabolic and signaling genes, as well as their functions in plant adaptation to drought. Under water deficit, CK signaling has evolved as an inter-cellular communication network which is essential to crosstalk with other types of phytohormones and their regulating pathways in mediating plant stress response. In this review, we revise the current understanding of CK involvement in drought stress tolerance. Particularly, a genetic framework for CK signaling and CK crosstalk with abscisic acid (ABA) in the precise monitoring of drought responses is proposed. In addition, the potential of endogenous CK alteration in crops towards developing drought-tolerant crops is also discussed.

show abstract

“…We documented that both tZ and cZ, which are isomers of zeatin, together with their derivatives are a major group of isoprenoid CKs in 7 DAP spikes. It has already been shown that trans-zeatin is the predominant form after anthesis (Morris et al 1993, Hess et al 2002), but comprehensive analysis of cytokinins during spike, spikelet, ovule and grain development has not yet been reported for wheat using LC-MS/MS (Chen et al 2019).…”

Section: Discussionmentioning

confidence: 99%

“…TaCKX6a02 was annotated as TaCKX2.1 , TaCKX6-D1 (JQ797673) was annotated as TaCKX2.2 and TaCKX2.4 was annotated as TaCKX2.2 . Annotations for these genes were maintained in the recently published review on the TaCKX (Chen et al 2019), however tested in this research TaCKX10 was renamed as TaCKX9 and TaCKX3 was renamed as TaCKX11 . Newly revised by Chen et al (2019) naming is applied and former names are in brackets.…”

Section: Introductionmentioning

confidence: 99%

Silencing ofTaCKX1mediates expression of otherTaCKXgenes to increase grain yield in wheat

Jabłoński

Ogonowska

Szala

et al. 2020

Preprint

View full text Add to dashboard Cite

TaCKX family genes influence development of wheat plants by specific regulation of cytokinin content in different organs. However, their detailed role is not known. The TaCKX1, highly and specifically expressed in developing spikes and in seedling roots, was silenced by RNAi-mediated gene silencing via Agrobacterium and the effect of silencing was investigated in 7 DAP spikes of T1 and T2 generations. Various levels of TaCKX1 silencing in both generations influence different models of co-expression with other TaCKX genes and parameters of yield-related traits. Only a high level of silencing in T2 resulted in strong down-regulation of TaCKX11 (3), up-regulation of TaCKX2.1, 2.2, 5 and 9 (10), and a high yielding phenotype. This phenotype is characterised by higher spike number, grain number and grain yield, as well as slightly higher mass of seedling roots, but lower thousand grain weight (TGW) and slightly lower spike length. Content of most of cytokinin forms in 7 DAP spikes of silenced T2 lines increased from 40 to 76% compared to the non-silenced control. The CKs cross talk with other phytohormones.Each of the tested yield-related traits is regulated by various up- or down-regulated TaCKX genes and phytohormones. Unexpectedly, increased expression of TaCKX2.1 in silent for TaCKX1 T2 plants up-regulated trans- and cis-zeatin and trans-zeatin glucosides, determining lower TGW and chlorophyll content in flag leaves but higher grain yield. The coordinated effect of TaCKX1 silencing on expression of other TaCKX genes, phytohormone levels in 7 DAP spikes and yield-related traits in silenced T2 lines is presented.One-sentence summaryDifferent levels of TaCKX1 silencing influence various models of coordinated expression of TaCKX genes and phytohormone levels in 7 DAP spikes, as well as yield parameters.

show abstract

Cytokinin dehydrogenase: a genetic target for yield improvement in wheat

Cited by 113 publications

References 127 publications

Different sets of TaCKX genes affect yield-related traits in wheat plants grown in a controlled environment and in field conditions

Different sets of TaCKX genes affect yield-related traits in wheat plants grown in a controlled environment and in field conditions

Role and Regulation of Cytokinins in Plant Response to Drought Stress

Silencing ofTaCKX1mediates expression of otherTaCKXgenes to increase grain yield in wheat

Contact Info

Product

Resources

About