Cytokinins production by fluorescent Pseudomonas isolated from rhizospheric soils of Malus and Pyrus

Abstract

Information on microbial production of cytokinins and their effect on plant growth are limited. Therefore, the objective of the present study was to investigate cytokinins production by Pseudomonas sp., a major component of rhizobacteria with multiform and diverse activities, which enhance plant growth by synthesizing various secondary metabolites. In the present investigations, thirty Pseudomonas isolates were isolated from the rhizosphere of Pyrus and Malus and were screened for cytokinins production (50 to 210 μg/ml). Four strains viz PN-4-SAN, PN-10-SAN, AN-2-NAG and AN-4-NAG were selected on the basis of their higher cytokinins production. The maximum cytokinins production was observed at 72h incubation period in nutrient broth at pH 7.0 under shaken condition at 28°C. Cytokinins were extracted, purified and evaluated by thin layer chromatography and specific bioassay method.

In vitro Screening and Optimization of IAA Production from Plant Growth Promoting Rhizobacteria Burkholderia cepacia UPMB3

“…2B). Ritika et al (2012) reported that different isolates of Pseudomonas sp produced auxins like substances in the stationary phase of growth at 72 hrs of incubation period at 28°C. The homogeneity of the partially purified auxins was checked by thin layer chromatography and auxins gave the maximum Rf value of 0.81 in solvent system isopropanol: water (3:2).…”

Mechanisms of Salt Stress Reduction in Plants Using Salt-Resistant Bacteria Isolated From Halophytes

“…Продукция ЦК является относительно обычным признаком PGPR и эндофитных бактерий. PGPR могут влиять на концентрацию ЦК в растении путем синтеза ЦК или изменения гомеостаза ЦК в растении [15]. Растения Platycladus orientalis, инокулированные штаммом PGPR B. subtilis, продуцирующим ЦК, имели повышенный уровень ЦК в побегах и были более устойчивыми к засухе [16].…”

Mechanistic Insights of the Interaction of Plant Growth-Promoting Rhizobacteria (PGPR) With Plant Roots Toward Enhancing Plant Productivity by Alleviating Salinity Stress

“…Cytokinin (CK) is another important class of phytohormones that assists plants in growth and development and in attributing resistance to different stresses (O'Brien and Benková, 2013). Though a common trait of PGPRs, they suffice plants of CK by either synthesizing it or altering its homeostasis in plants (Dodd et al, 2010;Pallai et al, 2012;Kapoor and Kaur, 2016). The inoculation of Pseudomonas sp.…”