Cytokinins Secreted by<i>Agrobacterium</i>Promote Transformation by Repressing a Plant Myb Transcription Factor

Sardesai, Nagesh; Lee, Lan‐Ying; Chen, Huabang; Yi, Hochul; Olbricht, Gayla R.; Stirnberg, Alexandra; Jeffries, Jacob; Xiong, Kia; Doerge, R. W.; Gelvin, Stanton B.

doi:10.1126/scisignal.2004518

Cited by 50 publications

(48 citation statements)

References 60 publications

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“…Previous reports on At14a described it as a PM protein (12,23,24), and AFL1 colocalized with FM4-64 [N-(3-Triethylammoniumpropyl)-4-(6-(4-(Diethylamino)phenyl)hexatrienyl)Pyridinium Dibromide] along the PM (Fig. 3A).…”

Section: Afl1 Colocalization With Clathrin Light Chain As Well As Strmentioning

confidence: 97%

“…Indeed, this may be related to AFL1, as cytokinin metabolism and signaling genes were differentially expressed in AFL1 O.E. plants (Dataset S1), and a connection of At14a to cytokinin effect on Agrobacteriummediated plant transformation was recently described (24).…”

Section: Discussionmentioning

confidence: 99%

“…The structural modeling raised questions of whether AFL1 is really a transmembrane protein, as previously suggested for At14a (23,24,27,28). Aqueous twophase partitioning found that AFL1 associated with both PM and endomembrane (Fig.…”

Section: Afl1 Is a Peripheral Membrane Protein Associated With Both Pmentioning

confidence: 99%

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At14a-Like1 participates in membrane-associated mechanisms promoting growth during drought in Arabidopsis thaliana

Kumar

Hsieh

Verslues

2015

Proc. Natl. Acad. Sci. U.S.A.

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Limited knowledge of how plants regulate their growth and metabolism in response to drought and reduced soil water potential has impeded efforts to improve stress tolerance. Increased expression of the membrane-associated protein At14a-like1 (AFL1) led to increased growth and accumulation of the osmoprotective solute proline without negative effects on unstressed plants. Conversely, inducible RNA-interference suppression of AFL1 decreased growth and proline accumulation during low water potential while having no effect on unstressed plants. AFL1 overexpression lines had reduced expression of many stress-responsive genes, suggesting AFL1 may promote growth in part by suppression of negative regulatory genes. AFL1 interacted with the endomembrane proteins protein disulfide isomerase 5 (PDI5) and NAI2, with the PDI5 interaction being particularly increased by stress. PDI5 and NAI2 are negative regulatory factors, as pdi5, nai2, and pdi5-2nai2-3 mutants had increased growth and proline accumulation at low water potential. AFL1 also interacted with Adaptor protein2-2A (AP2-2A), which is part of a complex that recruits cargo proteins and promotes assembly of clathrin-coated vesicles. AFL1 colocalization with clathrin light chain along the plasma membrane, together with predictions of AFL1 structure, were consistent with a role in vesicle formation or trafficking. Fractionation experiments indicated that AFL1 is a peripheral membrane protein associated with both plasma membrane and endomembranes. These data identify classes of proteins (AFL1, PDI5, and NAI2) not previously known to be involved in drought signaling. AFL1-predicted structure, protein interactions, and localization all indicate its involvement in previously uncharacterized membrane-associated drought sensing or signaling mechanisms.E ven relatively mild drought that causes reduced soil water potential (ψ w ) can result in dramatically reduced plant growth and agricultural productivity. Physiological analyses have shown that plant growth is actively down-regulated during drought and is not limited by carbon supply (1-3). Reductions of growth help ensure survival by conserving water but can be undesirable for agriculture, as plant productivity is reduced more than need be if growth were less sensitive to changes in water status (3). Also, specific metabolic pathways, such as proline metabolism, are stress regulated and contribute to drought tolerance.The sensing and signaling mechanisms controlling growth and metabolic responses to drought remain unclear. Many hypotheses of how plants sense water loss center on detection of mechanical stimuli generated by loss of turgor and cell shrinkage. This includes changes in membrane shape or disruption of cell wall-cell membrane connections possibly detected by proteins, such as mechanosensitive channels or receptor-like kinases that bind cell wall components (4-9). Proteins that induce or detect membrane curvature are known in mammalian cells (10) but have been little considered in plants. Also, in analogy to mammal...

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Section: Afl1 Colocalization With Clathrin Light Chain As Well As Strmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

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At14a-Like1 participates in membrane-associated mechanisms promoting growth during drought in Arabidopsis thaliana

Kumar

Hsieh

Verslues

2015

Proc. Natl. Acad. Sci. U.S.A.

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“…1C), suggesting that MTF1 influences plant growth and development. The importance of MTF1 in plant growth and development was strengthened by the observation that several different self-fertilized heterozygous mtf1 mutants which would encode less than 85% of the MTF1 protein failed to generate homozygous mutants, 1 indicating that loss of all MTF1 function is lethal. The mutant mtf1-4 would encode a protein ~85% of the length of the wild-type protein; homozygous mtf1-4 plants appear wild-type in growth and development.…”

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confidence: 99%

“…The mutant mtf1-4 would encode a protein ~85% of the length of the wild-type protein; homozygous mtf1-4 plants appear wild-type in growth and development. 1 The homozygous mutant mtf1-4 is highly susceptible to Agrobacterium infection. We tested the possibility that MTF1 is a general regulator of disease susceptibility by investigating whether mtf1-4 plants showed altered susceptibility to other pathogens.…”

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TheArabidopsisMyb transcription factor MTF1 is a unidirectional regulator of susceptibility toAgrobacterium

Sardesai

Laluk

Mengiste

et al. 2014

Plant Signaling & Behavior

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We recently described the Arabidopsis Myb transcription factor MTF1 that negatively regulates plant susceptibility to Agrobacteriummediated transformation. Roots of mtf1 mutant plants show increased susceptibility to several Agrobacterium strains, and complementing the mutants with a MTF1 cDNA decreases transformation susceptibility to wild-type levels. Here, we show that overexpression of MTF1 in a wild-type Arabidopsis background does not result in altered transformation susceptibility. However, MTF1 overexpressing plants show increased root length and larger and darker leaves, indicating that MTF1 plays a role in plant growth and development. MTF1 decreases Arabidopsis root susceptibility specifically to Agrobacterium but plant responses to the pathogens Alternaria brassicicola or Pseudomonas syringae pv Tomato were not altered. However, the homozygous MTF1 mutant mtf1-4 is resistant to Botrytis cinerea strain BO5-10 and is regulated through the ethylene signaling pathway mediated by upregulation of the AP2/ERF transcription factor ORA59.Over the past ~40 years, numerous studies have been conducted elucidating the molecular and genetic mechanisms of Agrobacterium-mediated plant transformation. However, only recently has the involvement of plant genes in the transformation process been investigated. Although specific plant genes mediate various steps of transformation, no gene that regulates the extent of transformation susceptibility had previously been described.We recently identified an Arabidopsis Myb transcription factor, MTF1, that plays a role in regulating Agrobacteriummediated transformation susceptibility. 1 mtf1 mutants show increased susceptibility to several Agrobacterium strains. In addition, decreasing MTF1 expression, using an RNAi approach, increases transformation susceptibility of a recalcitrant Arabidopsis ecotype. Ectopic overexpression of a MTF1 cDNA in the mtf1-4 homozygous mutant resulted in decreased transformation susceptibility of the resultant transgenic lines. Because decreased expression of MTF1 results in increased transformation susceptibility, we hypothesized that MTF1 is a "bidirectional regulator" such that decreased MTF1 expression would increase plant transformation susceptibility, whereas overexpression of MTF1 would decrease susceptibility to Agrobacterium-mediated transformation.We generated 5 MTF1 cDNA overexpression lines in wild-type Arabidopsis (ecotype Col-7) that showed a 9-to 43-fold increase in MTF1 transcript levels, as assessed by quantitative real-time reverse transcription PCR (Fig. 1A). We tested the transformation susceptibility of the overexpression line 4 (MTF-4) that had the highest MTF1 transcript level. There was no alteration in the transformation susceptibility of MTF-4 compared with that of wild-type plantsThe Arabidopsis Myb transcription factor MTF1 is a unidirectional regulator of susceptibility to Agrobacterium

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Advancement in Delivery Systems and Vector Selection for CRISPR/Cas‐Mediated Genome Editing in Plants

Vats,

Kaur,

Chauhan

et al. 2023

Applications of Genome Engineering in Plants

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Cytokinins Secreted byAgrobacteriumPromote Transformation by Repressing a Plant Myb Transcription Factor

Cited by 50 publications

References 60 publications

At14a-Like1 participates in membrane-associated mechanisms promoting growth during drought in Arabidopsis thaliana

At14a-Like1 participates in membrane-associated mechanisms promoting growth during drought in Arabidopsis thaliana

TheArabidopsisMyb transcription factor MTF1 is a unidirectional regulator of susceptibility toAgrobacterium

Advancement in Delivery Systems and Vector Selection for CRISPR/Cas‐Mediated Genome Editing in Plants

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