Cytolethal Distending Toxin Production, Genotypes and Atimicrobial Susceptibility of Campylobacter jejuni Isolates from Diarrhea Patients and Chickens

Abstract

Campylobacter jejuni isolates from diarrhea patients and chickens in 2008 in Iksan, Korea were tested for biochemical characteristics, and for possession of genes hipO, mutated gyrA, and cdtB. Among the chickens tested 52% carried C. jejuni. All 28 patient isolates and 48 chickens isolates had typical biochemical characteristics, except for nalidixic acid resistance. All isolates from patients and chickens were resistant to ciprofloxacin, and had mutated gyrA gene indicating good correlation of the two tests. Analysis of pulsed-field gel electrophoresis (PFGE) pattern of SmaI-restricted DNA of 53 isolates showed 14 clusters. Twenty-eight patient isolates and two chicken isolates (57%) showed an identical pattern (cluster 9). Chicken isolates C37 and C48 (cluster 2), C31 and C33 (cluster 3), C29, C34, C35, and C36 (cluster 4), and C43, C44 (cluster 6) had identical patterns. All patient isolates, compared to 87% and 80% of chicken isolates, were susceptible to amikacin and chloramphenicol, respectively. Antibiotics with the lowest MIC 90 were imipenem, gentamicin, and erythromycin, whereas, those with the highest were ampicillin and tetracycline. In conclusion, C. jejuni carriage rate of chickens in Iksan, Korea, was high, all 28 isolates from patients and two from chickens were an identical clone, whereas isolates from patients and remaining chickens were different clones with only 62% similarity, all isolates had hipO and cdtB genes, and all isolates were resistant to ciprofloxacin.

Genetic Properties and Antimicrobial Resistance of Campylobacter jejuni Isolates from Diarrhea Patients in Gyeonggi-do

“…C. jejuni 균을 동정하는 다른 방법으로는 독력 인자의 유무를 확인하는 것이다. 장염을 유발하는 독력인자는 명확하게 밝혀지지 않았지만 최근의 연구에 의하면 상피세포에 부착, 침입하는 능력, cytolethal distending toxin (CDT) 생성이 관여한다는 보고가 있다 (Kim et al, 2008). CDT 독소는 cdtA, cdtB, cdtC로 이루어져 있고, 그 중에서 C. jejuni에 의한 독성에 cdtB가 중요한 역할을 한다고 알려져 있다 (Purdy et al, 2000;Dassanayake et al, 2005).…”

“…CDT 독소는 cdtA, cdtB, cdtC로 이루어져 있고, 그 중에서 C. jejuni에 의한 독성에 cdtB가 중요한 역할을 한다고 알려져 있다 (Purdy et al, 2000;Dassanayake et al, 2005). 방글라데시 지역에서 분리한 C. jejuni의 97.5%에서 cdtB 유전자가 검출되었고, 우리나라 전북 지역에서 분리한 균의 경우 cdtB 유전자가 100% 검출되었다 (Kim et al, 2008;Talukder et al, 2008;Lindmark et al, 2009 (Zirnstein et al, 1999). (Gootz and Martin, 1991).…”

“…그러나 이외에 C. jejuni에 치료제로 쓰이는 macrolide계 항균 제인 erythromycin, azithromycin에는 41균주(97.6%)가 감수성 을 보였다 (Table 4) 그밖에 cephalothin에는 100%, tetracycline에는 69%가 내성 인 반면 imipenem에는 97.6%, chloramphenicol에는 100% 감 수성이었다 (Table 4). 이는 우리나라 전북지역과 부산지역에서 분리된 C. jeuni 항생제 내성 특징처럼 cephalothin과 tetracyclin (51.9%-93%)에는 높은 내성을, imipenem과 chloram phenicol 에는 높은 감수성을 보이는 결과와 비슷하다 (Park et al, 2007;Kim et al, 2008). …”