Cytological damage of nasal epithelium associated with decreased glutathione peroxidase in residents from a heavily polluted city

Hernández-Escobar, S. A.; Soto‐Abraham, Virgilia; Escudero, O. L. López; Soto, María Elena; Vega-Bravo, M. L.; Fortoul, Teresa I.; Reyes‐Maldonado, Elba

doi:10.1007/s00420-008-0378-1

Cited by 8 publications

(8 citation statements)

References 36 publications

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“…The decision to use nasal lavage method to sample the upper respiratory airways in this study was due to the fact that besides being a reproducible and well-tolerated procedure ) it is not invasive as bronchial biopsies and brochoalveolar lavage which require local anesthesia and a clinical setting. In addition, the nasal epithelium is the first to come into contact with inspired pollutants and therefore protects the lower airways (Hernández-Escobar et al 2009). Even though during highintensity exercise, such as the one performed in this study, athletes tend to breathe predominantly through their mouths, the NL technique is still useful to detect changes in the upper respiratory airways as shown in the ''Results'' section and elucidated further on.…”

Section: Discussionmentioning

confidence: 98%

Impact of heat and pollution on oxidative stress and CC16 secretion after 8 km run

2011

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To investigate the acute effect of a hot, humid and ozone-polluted (O(3)) environment on lung inflammation and oxidative tress of runners performing 8 km time trial run. Using a single-blinded randomized design, 10 male athletes (mean[Formula: see text]= 64.4 mlO(2) kg(-1) min(-1), SD = 4.4) took part in a time trial run in four different environmental conditions: 20°C + 50% relative humidity (rh) (Control); 20°C + 50% rh + 0.10 ppm O(3) (Control + O(3)); 31°C + 70% rh (Heat); 31°C + 70% rh + 0.10 ppm O(3) (Heat + O(3)). Blood samples and nasal lavage were collected post-exercise and analyzed for inflammatory, epithelial damage and oxidative stress markers. Data were analyzed using repeated measures ANOVA with Tukey's post hoc test. A significant increase in CC16 concentration (P < 0.05) and GSH/protein concentration (P < 0.05) in the upper respiratory airways was observed following the 8 km run in the Heat + O(3) trial compared with the control trial. There were no differences in the neutrophil counts between trials. No differences were observed for the other antioxidants analyzed. A hot, humid and ozone-polluted environment (0.1 ppm) elicits an early epithelial damage and antioxidant protection process in the upper respiratory airways of athletes immediately after performing 8 km time trial run.

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Section: Discussionmentioning

confidence: 98%

Impact of heat and pollution on oxidative stress and CC16 secretion after 8 km run

2011

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“…cGPx knockout mice exposed to noise showed significantly increased loss of hair cell and cochlear nerve fiber (Ohlemiller et al 2000). GPx concentration in human nasal scraping is associated with cytological damage of nasal epithelium induced by ozone exposure (Hernández-Escobar et al 2009). In the present study, pGPx mRNA was strongly expressed in neuroepithelial cells of the inner ear vestibule and olfactory epithelia of nasal cavity in embryos after E15.5, but pGPx protein showed a higher expression on all the periods examined until parturition.…”

Section: Discussionmentioning

confidence: 98%

Developmental expression of plasma glutathione peroxidase during mouse organogenesis

et al. 2011

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Plasma glutathione peroxidase (pGPx) is an extracellular antioxidative selenoenzyme which has been detected in various adult tissues, but little is known about the expression and distribution of pGPx during embryogenesis. To investigate the expression patterns of pGPx during embryogenesis, we performed quantitative real-time PCR, in situ hybridization, Western blot, and immunohistochemistry analyses in whole embryos or each developing organ of mice on embryonic days (E)7.5-18.5. In whole embryos of E7.5-8.5, pGPx mRNA was more typically expressed in extra-embryonic tissues including ectoplacental cone, trophectoderm, and decidual cells than in embryos. However, after E9.5, pGPx mRNA and protein levels were increased in the embryos with differentiation and growth, but trended to gradually decrease in the extra-embryonic tissues until E18.5. In sectioned embryonic tissues on E13.5-18.5, pGPx mRNA and protein were mainly expressed in the developing nervous tissues, the sensory organs, and the epithelia of lung, skin, and intestine, the heart and artery, and the kidney. In particular, pGPx immunoreactivity was very strong in the developing liver. These results indicate that pGPx is spatio-temporally expressed in various embryonic organs as well as extra-embryonic tissues, suggesting that pGPx may function to protect the embryos against endogenous and exogenous reactive oxygen species during organogenesis.

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“…This perturbation in natural antioxidant activity can result in endothelial damage especially if it were to occur during viral respiratory infections, as the host may be unable to repair oxidative damage caused by viral inflammation [5]. A similar process likely occurs in the nasal epithelium, as in situ glutathione levels and cytotoxic damage fluctuates in response to ozone and particulate matter [6]. …”

Section: Introductionmentioning

confidence: 99%

The association between acetaminophen and asthma

Kim¹,

Johnson²

2014

Current Opinion in Allergy &Amp; Clinical Immunology

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Purpose of review to examine literature evidence for the association between acetaminophen (paracetamol) use and development of rhinitis Recent findings Increased use of acetaminophen (paracetamol) as the favored antipyretic during pregnancy and infancy has been hypothesized to be a risk factor for the development of asthma. There is a paucity of well-designed birth cohort studies to examine paracetamol as a risk factor in the development of rhinitis. Confounding by antibiotic use, viral infections, and recall bias are problematic for many of the studies that are published. Summary Prospective birth cohorts need to dedicate adequate time and research personnel to adequately assess paracetamol exposure as a primary variable of interest rather than as an incidental exposure variable collected during routine questionnaires administration.

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Cytological damage of nasal epithelium associated with decreased glutathione peroxidase in residents from a heavily polluted city

Abstract: Cytological evaluation of nasal epithelium and GPX immunodetection are satisfactory methods to evaluate the earliest damage produced by atmospheric pollution in heavily contaminated cities.

Cited by 8 publications

References 36 publications

Impact of heat and pollution on oxidative stress and CC16 secretion after 8 km run

Impact of heat and pollution on oxidative stress and CC16 secretion after 8 km run

Developmental expression of plasma glutathione peroxidase during mouse organogenesis

The association between acetaminophen and asthma

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