Cytological examination of cerebrospinal fluid: Sysmex UF-1000i versus optical microscopy

Maleb, Adil; Bouayadi, Ouardia; Malki, J. El; Rifai, Safaa; Lamrabat, Somiya; Benaissa, Elmostafa; Lahlou, Yassine Ben; Frikh, Mohammed; Elouennass, M.

doi:10.1016/j.ab.2019.113501

Cited by 3 publications

(2 citation statements)

References 22 publications

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“…Diversos estudios han evaluado el grado de concordancia en los recuentos obtenidos con ambos analizadores y el método de referencia en cámara citométrica. En el caso del UF-1000i, Fleming et al [48] demostraron un buen acuerdo para el RBC-BF, pero solo aceptable para el WBC-BF, de forma similar al demostrado por Buoro et al [46] en muestras con recuentos <30 células/μL y más recientemente por Maleb et al [50]. En estos estudios, el analizador sobreestimó el WBC-BF.…”

Section: Analizadores Para Urianálisis Analizadores Sysmexunclassified

El recuento automatizado de células en líquidos biológicos: una revisión

Martín¹,

Queral²,

Frías³

et al. 2021

Advances in Laboratory Medicine / Avances en Medicina De Laboratorio

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Resumen El recuento de células en líquidos biológicos proporciona una información valiosa para el diagnóstico y tratamiento de diferentes patologías. El recuento en cámara y el estudio de la celularidad mediante microscopía óptica han sido consideradas tradicionalmente como método de referencia. Sin embargo, esta metodología implica un tiempo de respuesta del laboratorio elevado, carece de la reproducibilidad adecuada y requiere de personal experto. El avance tecnológico ha permitido el desarrollo de módulos de análisis específicos para los líquidos biológicos, incorporados en analizadores de hematología y de orinas, que permiten la automatización del recuento celular y han sido rápidamente incorporados a la práctica asistencial de los laboratorios En la actualidad diferentes analizadores están disponibles para ofrecer soluciones de automatización en el recuento de células en líquidos biológicos. Sin embargo, el empleo de dichos analizadores no está exento de limitaciones y su utilización requiere de un profundo conocimiento por los especialistas de la Medicina de Laboratorio. En esta revisión, se describen las principales tecnologías para la automatización del recuento celular en líquidos biológicos, el significado de los parámetros informados por los analizadores, sus principales características analíticas, así como el papel de la microscopía óptica en un contexto de utilización creciente de estas tecnologías.

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Section: Analizadores Para Urianálisis Analizadores Sysmexunclassified

El recuento automatizado de células en líquidos biológicos: una revisión

Martín¹,

Queral²,

Frías³

et al. 2021

Advances in Laboratory Medicine / Avances en Medicina De Laboratorio

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“…WBC count was done by Sysmex KX-21 automated hematology analyzer )Sysmex Corporation, Japan( and crosschecked by an improved Neubar chamber by using a light microscope. 13 The ACD is defined as elevated CSF albumin level )>45 mg/dL( along with normal white blood cell count )<5.0/µL(. 14 The ACD was found in 95 out of 110 GBS patients.…”

Section: Exclusion Criteriamentioning

confidence: 99%

Clinical and biochemical profile of Guillain Barré Syndrome in Pakistan

Iqbal

Asad

Shah

et al. 2021

NSJ

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The GBS patients were diagnosed through physical examination and nerve conduction study )NCS(. Hemoglobin )Hb(, liver function tests, renal function tests, lipid profile, and interleukin-17 levels were investigated through the blood. Cerebrospinal fluid analysis )CSF( was performed to measure albuminocytological dissociation )ACD(. Results:One hundred and ten patients and 130 controls participated in this study. Fifty-nine )53.63 %( patients had gastrointestinal infection while 52 )47.27 %( had AIDP. IL-17 serum levels were associated with GBS showing elevated values 18.49 pg/mL )SD=4.10( in cases as compared to controls 10.66 )SD=2.09(, p<0.001. The CSF proteins were statistically significant in GBS patients, 127.93 mg/dL )SD=51.28(, p=0.002. Ascending weakness was observed in 82 )74.5 %( patients. The results showed that the mean age value of GBS patients was 41.27 years, showing males preponderance. The mean Hb value for males and females was 14.83 g/dL and 10.88 g/dL respectively. Seasonal trends in the disease showed that 40% of GBS patients had infections during the spring. Conclusion:The results of the current study suggest that IL-17 levels trigger autoimmunity in GBS patients. The ACD could be used as a diagnostic marker of GBS along with NCS. Antecedent infections were common in a majority of GBS patients.

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Automated cell count in body fluids: a review

Martín

Queral

Frías

et al. 2021

Advances in Laboratory Medicine / Avances en Medicina De Laboratorio

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Body fluid cell counting provides valuable information for the diagnosis and treatment of a variety of conditions. Chamber cell count and cellularity analysis by optical microscopy are considered the gold-standard method for cell counting. However, this method has a long turnaround time and limited reproducibility, and requires highly-trained personnel. In the recent decades, specific modes have been developed for the analysis of body fluids. These modes, which perform automated cell counting, are incorporated into hemocytometers and urine analyzers. These innovations have been rapidly incorporated into routine laboratory practice. At present, a variety of analyzers are available that enable automated cell counting for body fluids. Nevertheless, these analyzers have some limitations and can only be operated by highly-qualified laboratory professionals. In this review, we provide an overview of the most relevant automated cell counters currently available for body fluids, the interpretation of the parameters measured by these analyzers, their main analytical features, and the role of optical microscopy as automated cell counters gain ground.

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Cytological examination of cerebrospinal fluid: Sysmex UF-1000i versus optical microscopy

Cited by 3 publications

References 22 publications

El recuento automatizado de células en líquidos biológicos: una revisión

El recuento automatizado de células en líquidos biológicos: una revisión

Clinical and biochemical profile of Guillain Barré Syndrome in Pakistan

Automated cell count in body fluids: a review

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