Cytological intracellular glycogen evaluation using PAS and PAS-D stains to correlate plasma glucose in diabetics

Ravikumar, S Shamala; Menaka, T R; Vasupradha, G; Dhivya, K; Dinakaran, J; Saranya, V

doi:10.4103/ijdr.ijdr_815_18

Cited by 5 publications

(4 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…During glycogen visualization via a traditional PAS reaction [ 20 ], it was found that OD values of the liver sections are reduced in LAP rats by 25.5% ( p = 0.0003) and by 32.1% ( p = 0.0001) in HAP animals compared to control ( Fig. 3 ).…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Phenotypically Determined Liver Dysfunction in a Wistar Rat Model of Post-Traumatic Stress Disorder

Kondashevskaya

Artem’eva

Алексанкина

et al. 2022

J Evol Biochem Phys

View full text Add to dashboard Cite

The extraordinary situation of the 2019–2022 pandemic caused a dramatic jump in the incidence of post-traumatic stress disorder (PTSD). PTSD is currently regarded not only as a neuropsychiatric disorder, but also as a comorbidity accompanied by cardiovascular diseases, circulatory disorders, liver dysfunction, etc. The relationship between behavioral disorders and the degree of morphofunctional changes in the liver remains obscure. In this study, PTSD was modeled in sexually mature male Wistar rats using predatory stress induced by a prey’s fear for a predator. Testing in an elevated plus maze allowed the rat population to be divided into animals with low-anxiety (LAP) and high-anxiety (HAP) phenotypes. It was found that morphofunctional analysis of the liver, in contrast to its biochemical profiling, provides a clearer evidence that predatory stress induces liver dysfunction in rats of both phenotypes. This may indicate a decrease in the range of compensatory adaptive reactions in stressed animals. However, in HAP rats, the level of morphofunctional abnormalities in the mechanisms responsible for carbohydrate-fat, water-electrolyte and protein metabolism in the liver testified the prenosological state of the organ, while further functional loading and resulting tension of the regulatory systems could lead to homeostatic downregulation. Meanwhile, the liver of LAP animals was only characterized by insignificant diffuse changes. Thus, we demonstrate here a link between behavioral changes and the degree of morphofunctional transformation of the liver.

show abstract

Section: Resultsmentioning

confidence: 99%

“…The use of the glycogen-specific PAS reaction [ 20 ] made it possible to detect a sharp decrease in the level of this energy storage material in HAP rats, and its far lesser depletion in LAP animals. A decrease in the OD of sections stained for glycogen was accompanied by a decrease in plasma CORT level (positive correlation).…”

Section: Discussionmentioning

confidence: 99%

Phenotypically Determined Liver Dysfunction in a Wistar Rat Model of Post-Traumatic Stress Disorder

Kondashevskaya

Artem’eva

Алексанкина

et al. 2022

J Evol Biochem Phys

View full text Add to dashboard Cite

show abstract

“…these studies provide findings consistent with the results of this study. The most common complications of open reduction and internal fixation include pain, swelling, trismus, paresthesia, and plate removal [ 10 , 11 , 12 ]. Additionally, neurosensory disturbances of the IAN can present as loss of vitality in affected teeth, with 81% of the affected teeth showing no response [ 13 ].…”

Section: Discussionmentioning

confidence: 99%

Incidence neurosensory deficit in mandibular fractures

Sneha

2023

Bioinformation

View full text Add to dashboard Cite

Sensory disturbances in the inferior alveolar nerve (IAN) prior to treatment can be attributed to various factors, including the site, type of fracture, and fracture displacement. Therefore, it is of interest to assess the incidence of inferior alveolar nerve injuries associated with mandibular fractures before and after surgical treatment. Group A consisted of patients with inferior alveolar nerve paresthesia before treatment, while Group B consisted of patients with inferior alveolar nerve paresthesia after treatment. A significant difference was observed between the two groups, with a p-value of 0.031 (p < 0.05) with the overall incidence of IAN deficit was 57.33% before treatment and 61.33% after treatment. These findings highlight the importance of promptly identifying and managing IAN injuries to minimize long-term consequences.

show abstract

“…Glycogenesis occurs with glucose entering the cell through glucose transporters (GLUT-1) which are tissuespecific. The entry of glucose into the cells is facilitated by two transport systems: insulin-dependent and insulinindependent glucose transport system [30] .…”

Section: Discussionmentioning

confidence: 99%

Placental Alterations of Gravidas with Vitamin D Deficiency: A Light and Electron Microscopic Study

El Sawy,

Desouky,

Ramadan

2023

Egyptian Journal of Histology

View full text Add to dashboard Cite

Introduction:Recently vitamin D showed greater importance in reproduction. Vitamin D affects cellular proliferation and differentiation, angiogenesis, fetal bone formation and immune modulation. There is increased number of pregnant women with vitamin D deficiency worldwide. Vitamin D deficiency (VDD) has been related to pregnancy complications such as preeclampsia, gestational diabetes mellitus and preterm birth. Objectives: To evaluate histological changes in placentas of vitamin D deficient mothers. Material and Methods: Twenty placentas of term live births were divided into two groups; normal vitamin D level (control) and VDD (n = 10 in each group). The morphology of placental villi of control group and VDD group were subjected to light microscopic (LM) examination using H&E, Masson's trichrome and PAS stains, and transmission electron microscopic (TEM) examination. Quantitative parameters of the placenta in the selected sections were estimated and statistically analyzed. Results: LM examination of placenta of VDD group showed collagen fibers deposition in the villous core, stromal edema, increased syncytial knots, irregularities and thickening of basement membrane of fetal capillaries, and deposition of fibrin around the villi. On TEM, syncytiotrophoblast of VDD group exhibited fragmented heterochromatic nuclei, dilated profiles of rough endoplasmic reticulum, disrupted mitochondria and disorganized and separated microvilli. Cytotrophoblasts of VDD group showed irregular nuclear outline among numerous intracytoplasmic vacuoles. Concerning the histopathological grading, the VDD group showed a higher score in trophoblast degenerative knots compared to control. Edema and collagen fibers deposition were present in VDD group in 70% and 60% of the examined sections respectively. Conclusion: Maternal VDD is likely to be responsible for placental barrier impairment that disrupts transplacental transport and exchange which could be associated with some pregnancy complications and might affect development of the fetus and even future life.

show abstract

Cytological intracellular glycogen evaluation using PAS and PAS-D stains to correlate plasma glucose in diabetics

Cited by 5 publications

References 12 publications

Phenotypically Determined Liver Dysfunction in a Wistar Rat Model of Post-Traumatic Stress Disorder

Phenotypically Determined Liver Dysfunction in a Wistar Rat Model of Post-Traumatic Stress Disorder

Incidence neurosensory deficit in mandibular fractures

Placental Alterations of Gravidas with Vitamin D Deficiency: A Light and Electron Microscopic Study

Contact Info

Product

Resources

About