Cytology of desmoplastic small round cell tumor

Crapanzano, John P.; Cardillo, Marina; Lin, Oscar; Zakowski, Maureen F.

doi:10.1002/cncr.10316

Cited by 54 publications

(61 citation statements)

References 22 publications

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“…Similar to reports of Zeppa et al [11], we did not detect in our smears fragments of fibrosis or cytoplasmic granules or vacuoles. The finding of stromal fragments, frequently seen in FNA is not a common finding in liquid based preparations [12]. …”

Section: Discussionmentioning

confidence: 99%

Desmoplastic small round cell tumour : Cytological and immunocytochemical features

et al. 2005

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BackgroundDesmoplastic small round cell tumor (DSRCT) is a rare and highly aggressive neoplasm. The cytological diagnosis of these tumors can be difficult because they show morphological features quite similar to other small round blue cells tumors. We described four cases of DSRCT with cytological sampling: one obtained by fine needle aspiration biopsy (FNAB) and three from serous effusions. The corresponding immunocytochemical panel was also reviewed.MethodsPapanicolaou stained samples from FNAB and effusions were morphologically described. Immunoreaction with WT1 antibody was performed in all cytological samples. An immunohistochemical panel including the following antibodies was performed in the corresponding biopsies: 34BE12, AE1/AE3, Chromogranin A, CK20, CK7, CK8, Desmin, EMA, NSE, Vimentin and WT1.ResultsThe smears showed high cellularity with minor size alteration. Nuclei were round to oval, some of them with inconspicuous nucleoli. Tumor cells are clustered, showing rosette-like feature. Tumor cells in effusions and FNA were positive to WT1 in 3 of 4 cytology specimens (2 out 3 effusions and one FNA). Immunohistochemical reactions for vimentin, NSE, AE1/AE3 and WT1 were positive in all cases in tissue sections.ConclusionThe use of an adjunct immunocytochemical panel coupled with the cytomorphological characteristics allows the diagnosis of DSRCT in cytological specimens.

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Section: Discussionmentioning

confidence: 99%

Desmoplastic small round cell tumour : Cytological and immunocytochemical features

et al. 2005

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“…Despite the fact that DSRCT is now a well-documented entity with several cytological reports in the literature, generally comprising case reports that include findings in both FNA material and ascitic/pleural effusions [6,7,8,9,17,18,19,20,21,22], its diagnosis is still usually established by histopathological examination.…”

Section: Discussionmentioning

confidence: 99%

“…Immunocytochemically, DSRCT demonstrates a divergent differentiation, a striking feature of this tumor [1,2,3,4,5,6]. Typically the tumor cells are immunoreactive for epithelial (low-molecular weight cytokeratins and epithelial membrane antigen), mesenchymal (vimentin and desmin) and neural (neuron-specific enolase and CD56) markers [4,10,11].…”

Section: Introductionmentioning

confidence: 99%

“…The tumor cells demonstrate a high nuclear-cytoplasmatic ratio, oval to round hyperchromatic nuclei with a finely granular chromatin pattern and inconspicuous nucleoli, smooth or irregular nuclear membranes and frequent nuclear molding. The cytoplasm is scant to moderate, palely amphophilic to eosinophilic and occasionally vacuolated [3,4,5,6,7,8,9]. …”

Section: Introductionmentioning

confidence: 99%

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Desmoplastic Small Round Cell Tumor: Diagnosis by Fine-Needle Aspiration Cytology

Leça

Vieira²,

Teixeira³

et al. 2012

Acta Cytologica

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Background: Desmoplastic small round cell tumor (DSRCT) is a distinctive clinicopathologic entity with an aggressive clinical course that typically involves the abdominal and/or pelvic peritoneum of young males. A population of small round blue cells and a fibroesclerotic stroma are the usual morphologic features. This tumor is characterized by a typical polyphenotypic profile with expression of epithelial, mesenchymal and neural markers. Cytogenetically, this tumor presents a unique abnormality – t(11;22)(p13;q12). Case: A 29-year-old male without significant medical history was admitted to our institution with gastrointestinal symptomatology. The physical examination and medical imaging studies revealed an extensive soft tissue mass filling the entire peritoneal cavity/pelvis. A fine-needle aspiration (FNA) of the abdominal mass was performed. The FNA smears revealed fragments of collagenous desmoplastic stroma and clusters of loosely cohesive small round cells that showed positivity for epithelial and myogenic markers. Cytogenetic analysis demonstrated rearrangement of the genes EWSR1 and WT1, resulting from the t(11;22)(p13;q12). Conclusion: DSRCT is an uncommon neoplasm that shares clinical and cytomorphologic features with other small round cell tumors. Therefore, a primary definitive diagnosis based on cytology specimens may be difficult but plausible and can be aided by a typical clinical presentation and ancillary immunocytochemical/cytogenetic studies.

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“…Pseudorosettes and nuclear molding can be seen. 17 In addition, perinuclear cytoplasmic densities and stromal fragments may be seen on direct smears. These features may be especially difficult to differentiate from IOT.…”

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confidence: 98%

Imprint cytology of high‐grade immature ovarian teratoma: A case report, literature review, and distinction from other ovarian small round cell tumors

Ramalingam

Teague

Reid-Nicholson

2008

Diagnostic Cytopathology

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Immature ovarian teratoma (IOT) is a rare and aggressive malignant neoplasm characterized by immature neural tissue. The cytomorphologic features have only rarely been described. We herein describe an additional case and review the literature regarding this entity. To the best of our knowledge, this is the first reported case with imprint cytology. A 35-year-old woman presented with a pelvic mass which was resected and sent for frozen section evaluation. Imprint smears and frozen section of the mass were diagnostic of IOT. IOT has diagnostic cytologic features which show complete concordance with histology. Differential diagnoses include other small round cell neoplasms such as ovarian neuroblastoma, small cell carcinoma of hypercalcemic type, primitive neuroectodermal tumor, Wilm's tumor, desmoplastic small round cell tumor, and Non-Hodgkin lymphoma. Distinguishing IOT from these tumors can be challenging however if diligent morphologic study and/or ancillary studies are performed accurate diagnosis is possible.

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Cytology of desmoplastic small round cell tumor

Cited by 54 publications

References 22 publications

Desmoplastic small round cell tumour : Cytological and immunocytochemical features

Desmoplastic small round cell tumour : Cytological and immunocytochemical features

Desmoplastic Small Round Cell Tumor: Diagnosis by Fine-Needle Aspiration Cytology

Imprint cytology of high‐grade immature ovarian teratoma: A case report, literature review, and distinction from other ovarian small round cell tumors

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