“…In fact, they made us disqualify several subjects' material (61.1%) for having a totally irregular distribution of chromogen results in diverse nucleus cells and a major intensity in a specific place, perhaps, due to the different fixing agents during impregnation method (Hanselaar et al, 1992). The usage of different fixing agents with different fixation methods shows some varieties about chromogen intensity in the ionic hydrogen concentration and the hydrolise time in Feulgen's reaction (Böhm;Böhm et al, 1968) that could interfere in the condensation of chromatin's spatial distribution and, apart from tracking the DNA, does the same with proteins (Stedman & Stedman, 1950), Umayahara et al (2002).…”