Cytophotometric analysis of corresponding cytological and histological cervical intraepithelial neoplasia grade III specimens

Abstract

Cytophotometric analysis of cervical intraepithelial neoplasia grade I11 (CIN 111) was performed in 22 cytological smears (CS) and in 22 corresponding cytospin specimens retrieved from selected areas of paraffin-embedded tissues (PEC). The average time interval between cytological and histological diagnosis was 6 weeks. CIN I11 nuclei in CS and PEC specimen were Thionin-Feulgen stained and digitized. Beside the visual classification of DNA ploidy patterns, the 2 . 5~ and 5c exceeding rates and the specimen mean and standard deviation values of 21 photometric features were also analyzed. It was shown that, although there was a significant correlation between DNA ploidy patterns in corresponding PEC and CS specimen, the DNA patterns were dissimilar in eight of 22 cases. The DNA index, as represented by 2 . 5~ and 5c exceeding rates, was significantly higher in the CS specimen. High-resolution cytophotometric analysis of cell nuclei in CS and PEC specimens showed significant differences for a large number of nuclear photometric features. These findings can possibly be explained by differences in selection of CIN I11 cells from CS and PEC specimens and by differences between fixation procedures as used for the two techniques. It was concluded that cytophotometric data of CS and PEC specimens representing CIN I11 lesions should not be regarded as interchangeable.

Utility of Residual AutoCyte Cervical Cytology Samples for Image Analysis

“…DNA profiling of cervical cytology and biopsy samples has been proposed by a number of investigators who have found such studies to be informative for classification of lesions 2,3,12,14,19,20 and for the predicition of progressive behavior. 4,18 Visual classification of profile histograms has been used as have derived parameters, such as the 2c deviation index and the 5c exceeding rate.…”

“…[2][3][4]12,14,16,19,20,22 Although the DNA profile values in conventional smears show a good correlation with those obtained from disaggregated cervical biopsies, lack of complete concordance has been noted. 20 The availability of liquid-based, thin-layer cytology presents the pathologist with the opportunity to obtain additional cells to examine in the case of ambiguous samples or for ancillary studies without committing the sole diagnostic slide to the procedure. We previously found such liquid-based samples to be stable for extended periods.…”

“…9 A particularly useful ancillary study in cervical cytology would be DNA profiling, as performed earlier. 13,18,20,26 The process of destaining these conventional smears and restaining with the Feulgenthionin procedure, however, leads to unpredictable losses in stainable content according to some investigators. 1 Other investigators have resorted to disaggregation of nuclei from subsequent cervical biopsies in order to investigate DNA ploidy of cervical lesions.…”

Study of numerical aberrations of chromosome 1 by fluorescent in situ hybridization and DNA content by densitometric analysis on (pre)-malignant cervical lesions

“…For cervical smears, a good discrimination between control samples and dysplasia can also be reached by determination of the DNA content (Atkin & Kay, 1979;Frielander et aI., 1984) by image analysis (Bibbo et al, 1985;Hanselaar et al, 1991;Deleener et aI., 1991). Several studies have demonstrated that the preneolastic stages showed a much higher DNA content (polyploidisation with subsequent aneuploidization) than the normal cervical smears (Bibbo et aI., 1985;Hanselaar et al, 1991;Deleener et al, 1991).…”

“…Several studies have demonstrated that the preneolastic stages showed a much higher DNA content (polyploidisation with subsequent aneuploidization) than the normal cervical smears (Bibbo et aI., 1985;Hanselaar et al, 1991;Deleener et al, 1991).…”