Cytoplasm

Gartner, Leslie P.; Hiatt, James L.

doi:10.1016/b978-1-4160-2945-8.50004-1

Cited by 57 publications

(86 citation statements)

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“…Glycogen synthesis and detoxification of drugs are performed in sER in the liver. Severely dilated ER is representative of severely damaged hepatocytes [26] . When we examined ERs of the rats treated with saline, we found serious damage as irregular lamellar organization, large dilatations and focal breaks in rERs, and vacuolizations and myelin figures in sERs in many areas.…”

Section: Discussionmentioning

confidence: 99%

“…Swelling and loss of regular cristae structure with rupture of outer membrane, which are characteristics of deteriorated function of mitochondria [22,24,25] , decreased with taurine in our study. Endoplasmic reticulum is normally composed of tubules, vesicles and sacs forming lamel-like shape [26] . Crucial pathways that are related to carbohydrate metabolism, biotransformation, steroid metabolism and protein processing function in the ER with integration to other organelles [27] .…”

Section: Discussionmentioning

confidence: 99%

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Ultrastructural changes in hepatocytes after taurine treatment in CCl4 induced liver injury

Taşçı

Mas²,

Mas³

et al. 2008

WJG

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AIM:To search the organelle based changes in hepatocytes after taurine treatment in experimental liver fibrosis induced by CCl 4 administration. METHODS: Thirty rats were divided into two groups. Group 1 (n = 15) was injected with CCl 4 plus taurine and Group 2 (n = 15) with CCl 4 plus saline for 12 wk. At the end of 12th wk, mitochondria, rough and smooth endoplasmic reticulum, and nuclei of hepatocytes were evaluated using a scoring system. The results were compared with histopathological findings, as well. RESULTS: Taurine treatment reduced fibrosis scores significantly as compared to placebo. Organelle injury scores decreased significantly with taurine treatment. Ultrastructural and histopathological scores in both groups were in strong correlation (r = 0.931 for CCl 4 plus taurine and r = 0.899 for CCl 4 plus saline group). CONCLUSION:Organelle based transmission electron microscopy findings can reflect successfully histological results as well as tissue healing in hepatocytes from hepatotoxin-induced liver fibrosis.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Ultrastructural changes in hepatocytes after taurine treatment in CCl4 induced liver injury

Taşçı

Mas²,

Mas³

et al. 2008

WJG

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“…This is in agreement with our data showing that cells receiving 360 mins Pluronic L61 or P85 alone was able to deplete ATP to a level that at which the addition of 15 mins heat showed no added effects. In the current study, L61, a highly hydrophobic polymer, has a PO block length (approximately 9.3 nm) comparable to the thickness of the cell membrane which is about 7–10 nm (60) and a very short hydrophilic block (0.7 nm) length. This may facilitate its interaction with the lipid bilayer, such as increased lipid “flip-flop” action of the membrane (61), hence reaching the site of action with more ease, and leading to a more potent thermal sensitizing effect (54, 62).…”

Section: Discussionmentioning

confidence: 77%

Time and Dose Dependence of Pluronic Bioactivity in Hyperthermia-Induced Tumor Cell Death

Krupka

Dremann

Exner

2009

Exp Biol Med (Maywood)

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Pluronic block copolymers have been shown to sensitize cancer cells resulting in an increased activity of antineoplastic agents. In the current study we examined a new application of Pluronic bioactivity in potentiating hyperthermia-induced cancer cell injury. DHD/K12/TRb rat adenocarcinoma cells were exposed to low-grade hyperthermia at 43°C with or without Pluronic P85 or Pluronic L61. A range of Pluronic doses, pre-exposure and heat exposure durations were investigated, and the test conditions were optimized. Treatment efficacy was assessed by measurement of intracellular ATP and mitochondrial dehydrogenase activity. Both P85 and L61 in synergy with heat reduced cell viability appreciably compared to either heat or Pluronic alone. Under optimal conditions, P85 (10 mg/ml, 240 mins) combined with 15 mins heat reduced intracellular ATP to 60.1 ± 3.5% of control, while heat alone and P85 without heat caused a negligible decrease in ATP of 1.2% and 3.8%, respectively. Similarly, cells receiving 120 mins pre-exposure of L61 (0.3 mg/ml) showed reduction in intracellular ATP to 14.1 ± 2.1% of control. Again, heat or L61 pre-exposure alone caused a minor decrease in levels of intracellular ATP (1.5% and 4.4%, respectively). Comparable results were observed when viability was assessed by mitochondrial enzyme activity. Survival studies confirmed that the loss of viability translates to a long-term reduction in proliferative activity, particularly for L61 treated cells. Based on these results, we conclude that Pluronic is effective in improving hyperthermic cancer treatment in vitro by potentiating heat-induced cytotoxicity in a concentration and time dependent manner.

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“…Fe HX is commonly used to detect cytoskeletal elements in different types of nervous (Gharravi et al, 2007; Tubbs et al, 2015) and muscular tissues including SMF (Gartner & Hiatt, 2006), cardiac (Abdelhadi et al, 2015; Gartner, 2020), and skeletal muscles (El Dawi et al, 2013).…”

Section: Discussionmentioning

confidence: 99%