Cytoprotective Activity of Ethanol Fraction of Coleus Amboinicus Lour. Leaves Against Vero Cells Induced by H2o2

Haryani, Reny; Harahap, Urip; Masfria, Masfria; Satria, Denny

doi:10.22159/ajpcr.2018.v11s1.26559

Cited by 3 publications

(4 citation statements)

References 6 publications

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“…MTT method is a quantitative analysis of viable cell which conducted to the amount of formazan crystal where life cells reduce MTT salts [19], [20], [21], [22].…”

Section: Discussionmentioning

confidence: 99%

“…MTT Assay. Cytoprotective activity of EAF against H 2 O 2 (0.8 mM) induced oxidative stress was determined by MTT assay based on [19], [20]. The data which were absorbed from each well were converted to percentage of viable cells.…”

Section: Preparation Of Ethylacetate Fraction (Eaf)mentioning

confidence: 99%

“…6 cells/well) were seeded into 6-well plate and incubated for 24 h. After that, the cells were treated with extract and then incubated for 24 h. Each of well was added 10 µL DCFDA and incubated for 30 m, and then 1000 µL medium with or without H 2 O 2 (0.8 mM) was added and incubated for 3h. Cells were harvested and analyzed based on previous study [17], [18], [19], [20], [21].…”

Section: Ros Expression Analysis Vero Cells (1x10mentioning

confidence: 99%

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Cytoprotective Activity of Ethylacetate Fraction of Picria fel-terrae Lour. Herbs

Satria¹,

Silalahi²,

Haro³

et al. 2019

Open Access Maced J Med Sci

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AIM: Free radicals produce from metabolism or enviromental which interact continously with biological system. Picria fel-terrae Lour. herbs have been used as antioxidant and treat various diseases. The aim of this study was to evaluate cytoprotective activity of ethylacetate fraction (EAF) of Picria fel-terrae Lour. herbs. METHODS: Cytoprotective activity were determined by MTT asay and flow cytometry assay on Vero cells which induced with H2O2 0.8 mM. RESULTS: EAF at 100 µg/mL were showed highest viability (88.83 ± 2.90%) and ROS expression (66.75%) on Vero cells. CONCLUSION: EAF of Picria fel-terrae Lour. herbs have cytoprotective activity.

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“…MTT method is a quantitative analysis of viable cell which conducted to the amount of formazan crystal where life cells reduce MTT salts [19], [20], [21], [22].…”

Section: Discussionmentioning

confidence: 99%

Section: Preparation Of Ethylacetate Fraction (Eaf)mentioning

confidence: 99%

Section: Ros Expression Analysis Vero Cells (1x10mentioning

confidence: 99%

See 1 more Smart Citation

Cytoprotective Activity of Ethylacetate Fraction of Picria fel-terrae Lour. Herbs

Satria¹,

Silalahi²,

Haro³

et al. 2019

Open Access Maced J Med Sci

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“…In a study by Sahrial and Solfaine (2019) [ 332 ], the authors noted that the ethanolic extract of C. amboinicus administered to Wistar rats with cisplatin-induced nephropathy inhibited pathological lesions by regulating the renal expression of TGF-1β in areas containing renal glomeruli and tubules. Furthermore, C. amboinicus has shown to exhibit moderate to high antibacterial [ 66 , 67 , 333 , 334 ], antioxidant/cytoprotective [ 333 , 335 , 336 ], and anticancer [ 336 , 337 ] activity.…”

Section: Traditional Usesmentioning

confidence: 99%

The Botanical, Chemical and Ethnobotanical Diversity of Southern African Lamiaceae

Rattray

Wyk

2021

Molecules

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The Lamiaceae is undoubtedly an important plant family, having a rich history of use that spans the globe with many species being used in folk medicine and modern industries alike. Their ability to produce aromatic volatile oils has made them valuable sources of materials in the cosmetic, culinary, and pharmaceutical industries. A thorough account of the taxonomic diversity, chemistry and ethnobotany is lacking for southern African Lamiaceae, which feature some of the region’s most notable medicinal and edible plant species. We provide a comprehensive insight into the Lamiaceae flora of southern Africa, comprising 297 species in 42 genera, 105 of which are endemic to the subcontinent. We further explore the medicinal and traditional uses, where all genera with documented uses are covered for the region. A broad review of the chemistry of southern African Lamiaceae is presented, noting that only 101 species (34%) have been investigated chemically (either their volatile oils or phytochemical characterization of secondary metabolites), thus presenting many and varied opportunities for further studies. The main aim of our study was therefore to present an up-to-date account of the botany, chemistry and traditional uses of the family in southern Africa, and to identify obvious knowledge gaps.

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Technological prospection of the biological activities of Plectranthus amboinicus (Loureiro) Sprengel: an integrative review

Borba

Ferreira

Mubárack

et al. 2021

RSD

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Plectranthus amboinicus (Loureiro) Sprengel is popularly known as malvariço or indian borage. This paper carries out the technological prospecting of this species with an emphasis on its biological activities. The research consisted of an integrative literature review based on information collected from patent and electronic databases. Initially, 3019 results were obtained, which, after the screening, selection and analysis process, resulted in 55 articles and 48 patent documents. It was found that 77.08% (n=37) of patent deposits refer to obtaining or improving the process and 22.92% (n=11) to the finished product related to the following indications: wound healing, mainly in diabetics, followed by treatment of rheumatoid arthritis, hepatocellular carcinomas, breast cancer and melanoma, treatment for acne caused by P. acnes, S. aureus and P. aeruginosa, oral cavity cleansing agent, treatment for osteoporosis, nutraceutical composition for lactation and insect repellent. In the databases of scientific articles, 55 documents were selected, proving the following actions: antimicrobial, antioxidant, anticarcinogenic, anti-inflammatory, diuretic, nephroprotective, larvicide, analgesic, veterinary food additive, anti-leishmania, antipsoriasis, antidiarrheal, antidiabetic, hepatoprotective and anthelmintic. The scientific studies analyzed reaffirm other actions reported in popular use, but which are not described in the patent processes. Therefore, there are many proven biological properties of P. amboinicus that should be further explored and exploited in the pharmaceutical, cosmetic and food sectors in order to generate new products and patents.

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Cytoprotective Activity of Ethanol Fraction of Coleus Amboinicus Lour. Leaves Against Vero Cells Induced by H2o2

Cited by 3 publications

References 6 publications

Cytoprotective Activity of Ethylacetate Fraction of Picria fel-terrae Lour. Herbs

Cytoprotective Activity of Ethylacetate Fraction of Picria fel-terrae Lour. Herbs

The Botanical, Chemical and Ethnobotanical Diversity of Southern African Lamiaceae

Technological prospection of the biological activities of Plectranthus amboinicus (Loureiro) Sprengel: an integrative review

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