Cytoprotective Effects of Polyenoylphosphatidylcholine (PPC) on β-cells During Diabetic Induction by Streptozotocin

Abstract

Polyenoylphosphatidylcholine (PPC), a phosphatidylcholine-rich phospholipid extracted from soybean, has been reported to protect liver cells from alloxan-induced cytotoxicity. The present study aimed to investigate whether PPC protects pancreatic beta-cells from the cytotoxic injury induced by streptozotocin, thus preserving insulin synthesis and secretion. beta-Cells of the PPC-treated rats showed a significant reduction of cell death with lesser destruction of plasma membrane on streptozotocin insult. They demonstrated a rapid recovery of GLUT-2 expression, whereas almost irreversible depletion of membrane-bound GLUT-2 was seen in beta-cells of the rats treated with only streptozotocin. A similar cytoprotective effect of PPC was also monitored in the PPC-pretreated MIN6 cells. These beta-cells retained their ability to synthesize and secrete insulin and no alteration of glucose metabolism was detected. These results strongly suggest that PPC plays important roles not only in protecting beta-cells against cytotoxicity but also in maintaining their insulin synthesis and secretion for normal glucose homeostasis.

Physiological and Histological Studies on the Effect of Lupine Seeds on the Fish, Clarias lazera Treated with Glucose and Alloxan

“…However, all observed histological, ultrastructural and histochemical changes in the fish liver treated with alloxan or glucose were restored to normal state after administration of ALSS. This result is in harmony with that reported in alloxan-diabetic rats treated with lupine seeds (Abdel-Aal et al 1993) and in streptozotocin-diabetic rats treated with extracted substance from soybean (Lee et al, 2003). Such ameliorating effect of ALSS may be due to a cytotoxic resistance of ALSS against alloxan in the fish.…”

“…Also, the activity of b-cells was maintained in streptozotocin-treated rats fed soybean (Lee and Park 2000). Lee et al (2003) could extract the effective substance (polyenolphosphatidyl choline) from soybean which protects b-cells against cytotoxicity of diabetogenic agent streptozotocin as well as it stimulates insulin synthesis and secretion for normal glucose homeostasis. In the fish Onchorhynchus mykiss, soybean proteins in the diet (20-30%) can provide partial replacement for fish meal (Burrells et al 1999).…”

Ameliorative Anti-Diabetic Activity ofDangnyosoko, a Chinese Herbal Medicine, in Diabetic Rats

“…The anti-diabetic activity of extracts of Nigella sativa 28) and certain chemicals 29,30) has been detected based on the immunohistochemical changes in insulinand glucagon-producing cells. Insulin-producing cells are generally located in the central regions and glucagon-producing cells are in peripheral regions of the pancreatic islets, and more numerous insulin-producing cells are detected than glucagon-producing cells in the vertebra.…”

Impact of Extracellular Calcium Along With Ascorbic Acid on Cellular Reactivation and Insulin Secretion in Diabetic Min6 Cells

“…STZ treatment was excluded for positive control experiment. The cells were further tested for viability 10 .…”