Cytoprotective Peptides from Blue Mussel Protein Hydrolysates: Identification and Mechanism Investigation in Human Umbilical Vein Endothelial Cells Injury

Abstract

Cardiovascular disease represents a leading cause of mortality and is often characterized by the emergence of endothelial dysfunction (ED), a physiologic condition that takes place in the early progress of atherosclerosis. In this study, two cytoprotective peptides derived from blue mussel chymotrypsin hydrolysates with the sequence of EPTF and FTVN were purified and identified. Molecular mechanisms underlying the cytoprotective effects against oxidative stress which lead to human umbilical vein endothelial cells (HUVEC) injury were investigated. The results showed that pretreatment of EPTF, FTVN and their combination (1:1) in 0.1 mg/mL significantly reduced HUVEC death due to H2O2 exposure. The cytoprotective mechanism of these peptides involves an improvement in the cellular antioxidant defense system, as indicated by the suppression of the intracellular ROS generation through upregulation of the cytoprotective enzyme heme oxygenase-1. In addition, H2O2 exposure triggers HUVEC damage through the apoptosis process, as evidenced by increased cytochrome C release, Bax protein expression, and the elevated amount of activated caspase-3, however in HUVEC pretreated with peptides and their combination, the presence of those apoptotic stimuli was significantly decreased. Each peptide showed similar cytoprotective effect but no synergistic effect. Taken together, these peptides may be especially important in protecting against oxidative stress-mediated ED.

Nutrition and Cardiovascular Disease: The Potential Role of Marine Bioactive Proteins and Peptides in Thrombosis Prevention

“…41 Suryaningtyas et al identified cytoprotective peptides (EPTF and FTVN) from blue mussel chymotrypsin hydrolysates, reducing human umbilical vein endothelial cell (HUVEC) death caused by H 2 O 2 exposure. 42 In addition to fish and shellfish, other marine organisms have been rarely studied. Koyama et al isolated an anticoagulant peptide from starfish (Acanthaster planci) inhibiting the prothrombinase complex.…”

“…This anticoagulant protein inhibits the conversion of factor II (FII) to factor IIa (FIIa) in the blood coagulation cascade . Suryaningtyas et al identified cytoprotective peptides (EPTF and FTVN) from blue mussel chymotrypsin hydrolysates, reducing human umbilical vein endothelial cell (HUVEC) death caused by H 2 O 2 exposure . In addition to fish and shellfish, other marine organisms have been rarely studied.…”

Bioactive Peptides from Meretrix lusoria Enzymatic Hydrolysate as a Potential Treatment for Obesity in db/db Mice

“…Bioactive peptides are naturally occurring compounds that have gained interest as a promising area of study owing to their potential antioxidant, anti-obesity, and anti-diabetic properties, as well as their diverse modes of action. Bioactive peptides are short amino acid sequences derived from dietary proteins [ 9 , 10 , 11 ]. They facilitate targeting and interactions with biological processes and receptors associated with lipid metabolism, appetite regulation, and adipogenesis.…”

Anti‐inflammatory action of ark shell ( Scapharca subcrenata ) protein hydrolysate in LPS‐stimulated RAW264.7 murine macrophages

“…Recently, numerous bioactive peptides (BAPs) and certain proteins in food have been explored, and marine organisms are considered as a good source of protein and BAPs with potential biological activities such as antioxidant, antihypertensive, and anti‐microbial effects (Abad et al, 2011; Abdelhedi & Nasri, 2019; Balakrishnan et al, 2014; Cheung et al, 2016; Folmer et al, 2010; Khalifa et al, 2019; Semreen et al, 2018; Sgarbieri, 2017; Suryaningtyas et al, 2021; Zhong et al, 2019). As seen with their role in numerous health benefits, there have been tremendous attempts to develop BAPs with certain bioactivities.…”