Cytosolic glutathione peroxidase from liver of pacu (Piaractus mesopotamicus), a hypoxia-tolerant fish of the Pantanal

Bastos, V. L. F. Cunha; Salles, João Bosco de; Valente, Richard H.; León, Ileana R.; Perales, Jonás; Dantas, Rafael Ferreira; Albano, Rodolpho Mattos; Bastos, Frederico F.; Bastos, J. Cunha

doi:10.1016/j.biochi.2007.04.003

Cited by 42 publications

(16 citation statements)

References 43 publications

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“…In contrast to indigenous species from the Pantanal, L. fortunei lacks special adaptations to resist the low O 2 conditions during the ‘dequada’. The Pacu fish ( Piaractus mesopotamicus ) for example, has a peroxide-depredate enzyme (GPx) with enhanced basal activity that helps it overcome oxidative stress [29]. C. gigas genome by Zhang et al (2012) showed no expansion in gene families related to the antioxidant system.…”

Section: Discussionmentioning

confidence: 99%

Gene Discovery through Transcriptome Sequencing for the Invasive Mussel Limnoperna fortunei

Uliano-Silva

Americo

Brindeiro³

et al. 2014

PLoS ONE

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The success of the Asian bivalve Limnoperna fortunei as an invader in South America is related to its high acclimation capability. It can inhabit waters with a wide range of temperatures and salinity and handle long-term periods of air exposure. We describe the transcriptome of L. fortunei aiming to give a first insight into the phenotypic plasticity that allows non-native taxa to become established and widespread. We sequenced 95,219 reads from five main tissues of the mussel L. fortunei using Roche’s 454 and assembled them to form a set of 84,063 unigenes (contigs and singletons) representing partial or complete gene sequences. We annotated 24,816 unigenes using a BLAST sequence similarity search against a NCBI nr database. Unigenes were divided into 20 eggNOG functional categories and 292 KEGG metabolic pathways. From the total unigenes, 1,351 represented putative full-length genes of which 73.2% were functionally annotated. We described the first partial and complete gene sequences in order to start understanding bivalve invasiveness. An expansion of the hsp70 gene family, seen also in other bivalves, is present in L. fortunei and could be involved in its adaptation to extreme environments, e.g. during intertidal periods. The presence of toll-like receptors gives a first insight into an immune system that could be more complex than previously assumed and may be involved in the prevention of disease and extinction when population densities are high. Finally, the apparent lack of special adaptations to extremely low O2 levels is a target worth pursuing for the development of a molecular control approach.

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Section: Discussionmentioning

confidence: 99%

Gene Discovery through Transcriptome Sequencing for the Invasive Mussel Limnoperna fortunei

Uliano-Silva

Americo

Brindeiro³

et al. 2014

PLoS ONE

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“…Fifty micrograms of each venom were subjected to trypsin digestion, as previously described [53]. The tryptic digests were desalted with in-lab-generated columns packed with Poros R2 resin (Life Technologies, USA).…”

Section: Methodsmentioning

confidence: 99%

Comparison of Phylogeny, Venom Composition and Neutralization by Antivenom in Diverse Species of Bothrops Complex

Sousa

Nicolau

Peixoto³

et al. 2013

PLoS Negl Trop Dis

146

103

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In Latin America, Bothrops snakes account for most snake bites in humans, and the recommended treatment is administration of multispecific Bothrops antivenom (SAB – soro antibotrópico). However, Bothrops snakes are very diverse with regard to their venom composition, which raises the issue of which venoms should be used as immunizing antigens for the production of pan-specific Bothrops antivenoms. In this study, we simultaneously compared the composition and reactivity with SAB of venoms collected from six species of snakes, distributed in pairs from three distinct phylogenetic clades: Bothrops, Bothropoides and Rhinocerophis. We also evaluated the neutralization of Bothrops atrox venom, which is the species responsible for most snake bites in the Amazon region, but not included in the immunization antigen mixture used to produce SAB. Using mass spectrometric and chromatographic approaches, we observed a lack of similarity in protein composition between the venoms from closely related snakes and a high similarity between the venoms of phylogenetically more distant snakes, suggesting little connection between taxonomic position and venom composition. P-III snake venom metalloproteinases (SVMPs) are the most antigenic toxins in the venoms of snakes from the Bothrops complex, whereas class P-I SVMPs, snake venom serine proteinases and phospholipases A2 reacted with antibodies in lower levels. Low molecular size toxins, such as disintegrins and bradykinin-potentiating peptides, were poorly antigenic. Toxins from the same protein family showed antigenic cross-reactivity among venoms from different species; SAB was efficient in neutralizing the B. atrox venom major toxins. Thus, we suggest that it is possible to obtain pan-specific effective antivenoms for Bothrops envenomations through immunization with venoms from only a few species of snakes, if these venoms contain protein classes that are representative of all species to which the antivenom is targeted.

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“…40 kDa band (Figure 1A-I) and the low molecular mass (less than 10 kDa) band (Figure 1A-II) detected by SDS-PAGE analysis of the protein purified in the previous step were submitted to tryptic digestion [45]. The eluted peptides were completely dried on a SpeedVac concentrator (Thermo Scientific, Waltham, MA, USA), resuspended in 12 μl of a 1% (v/v) formic acid solution and kept at −20°C until mass spectrometric analyses, described below.…”

Section: Methodsmentioning

confidence: 99%

Isolation and molecular characterization of a major hemolymph serpin from the triatomine, Panstrongylus megistus

et al. 2014

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BackgroundChagas disease kills 2.5 thousand people per year of 15 million persons infected in Latin America. The disease is caused by the protozoan, Trypanosome cruzi, and vectored by triatomine insects, including Panstrongylus megistus, an important vector in Brazil. Medicines treating Chagas disease have unpleasant side effects and may be ineffective, therefore, alternative control techniques are required. Knowledge of the T. cruzi interactions with the triatomine host needs extending and new targets/strategies for control identified. Serine and cysteine peptidases play vital roles in protozoan life cycles including invasion and entry of T. cruzi into host cells. Peptidase inhibitors are, therefore, promising targets for disease control.MethodsSDS PAGE and chromatograpy detected and isolated a P. megistus serpin which was peptide sequenced by mass spectrometry. A full amino acid sequence was obtained from the cDNA and compared with other insect serpins. Reverse transcription PCR analysis measured serpin transcripts of P. megistus tissues with and without T. cruzi infection. Serpin homology modeling used the Swiss Model and Swiss-PDB viewer programmes.ResultsThe P. megistus serpin (PMSRP1) has a ca. 40 kDa molecular mass with 404 amino acid residues. A reactive site loop contains a highly conserved hinge region but, based on sequence alignment, the normal cleavage site for serine proteases at P1-P1′ was translocated to the putative position P4′-P5′. A small peptide obtained corresponded to the C-terminal 40 amino acid region. The secondary structure of PMSRP1 indicated nine α-helices and three β-sheets, similar to other serpins. PMSRP1 transcripts occurred in all tested tissues but were highest in the fat body and hemocytes. Levels of mRNA encoding PMSRP1 were significantly modulated in the hemocytes and stomach by T. cruzi infection indicating a role for PMSRP1 in the parasite interactions with P. megistus.ConclusionsFor the first time, a constitutively expressed serpin has been characterized from the hemolymph of a triatomine. This opens up new research avenues into the roles of serine peptidases in the T. cruzi/P. megistus association. Initial experiments indicate a role for PMSRP1 in T. cruzi interactions with P. megistus and will lead to further functional studies of this molecule.

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Cytosolic glutathione peroxidase from liver of pacu (Piaractus mesopotamicus), a hypoxia-tolerant fish of the Pantanal

Cited by 42 publications

References 43 publications

Gene Discovery through Transcriptome Sequencing for the Invasive Mussel Limnoperna fortunei

Gene Discovery through Transcriptome Sequencing for the Invasive Mussel Limnoperna fortunei

Comparison of Phylogeny, Venom Composition and Neutralization by Antivenom in Diverse Species of Bothrops Complex

Isolation and molecular characterization of a major hemolymph serpin from the triatomine, Panstrongylus megistus

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