2012
DOI: 10.1105/tpc.111.094946
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Cytosolic Glyceraldehyde-3-Phosphate Dehydrogenases Interact with Phospholipase Dδ to Transduce Hydrogen Peroxide Signals in the Arabidopsis Response to Stress

Abstract: Reactive oxygen species (ROS) are produced in plants under various stress conditions and serve as important mediators in plant responses to stresses. Here, we show that the cytosolic glycolytic enzymes glyceraldehyde-3-phosphate dehydrogenases (GAPCs) interact with the plasma membrane-associated phospholipase D (PLDd) to transduce the ROS hydrogen peroxide (H 2 O 2 ) signal in Arabidopsis thaliana. Genetic ablation of PLDd impeded stomatal response to abscisic acid (ABA) and H 2 O 2 , placing PLDd downstream o… Show more

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Cited by 209 publications
(211 citation statements)
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“…We also identified known PA-binding proteins, such as annexin, a PA-binding protein found in animals (35), and some isoforms of heat shock protein (Hsp81-3), and tubulins (␣2/4) previously identified (36). Among the protein candidates identified, GAPDH was particularly of interest because it was recently found to interact directly with PLD␦ (29), an enzyme responsible for PA production. In addition, GAPDH is one of the key enzymes involved in the glycolytic pathway that is critical for energy production and biosynthesis for various compounds, including acetyl-CoA and lipids (Fig.…”
Section: Resultsmentioning
confidence: 99%
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“…We also identified known PA-binding proteins, such as annexin, a PA-binding protein found in animals (35), and some isoforms of heat shock protein (Hsp81-3), and tubulins (␣2/4) previously identified (36). Among the protein candidates identified, GAPDH was particularly of interest because it was recently found to interact directly with PLD␦ (29), an enzyme responsible for PA production. In addition, GAPDH is one of the key enzymes involved in the glycolytic pathway that is critical for energy production and biosynthesis for various compounds, including acetyl-CoA and lipids (Fig.…”
Section: Resultsmentioning
confidence: 99%
“…In addition, our recent result indicates that oxidized GAPCs bind stronger to PLD␦ (29). To test in what state GAPC binds to PA, we performed a PA-GAPC binding assay in the presence of hydrogen peroxide (H 2 O 2 ) and DTT as an oxidant and reductant, respectively.…”
Section: Resultsmentioning
confidence: 99%
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