Cytotoxic activities of Hypericum perforatum L. extracts against 2D and 3D cancer cell models

Matić, Ivana Z.; Ergün, Sercan; Crnogorac, Marija Đorđić; Mısır, Sema; Aliyazıcıoğlu, Yüksel; Damjanović, Ana; Hurija, Džudžević-Čančar; Stanojković, Tatjana; Konanç, Kalbiye; Petrović, Nina

doi:10.1007/s10616-021-00464-5

Cited by 10 publications

(14 citation statements)

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“…In 2008, a review by Cochrane concluded that the results using plant extracts of HP were superior to placebo in patients with major depression, and were equally effective as standard antidepressants with fewer side effects [ 22 , 23 ]. Also, antioxidant, vasoprotective, anticarcinogenic, and antiviral properties of HP have been described [ 24 , 25 ].…”

Section: Discussionmentioning

confidence: 99%

Hypericum perforatum and Its Potential Antiplatelet Effect

et al. 2022

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Background: Hypericum perforatum (HP) is currently one of the most consumed medicinal plants in the world. In traditional Chinese medicine, the herb hypericum ( Guan Ye Lian Qiao ) belongs to the group of plants that clarify heat. It is also used to treat various types of infection and inflammation. In contrast to the extensive literature on the antidepressant effects of HP, little is known about its action on platelets. The main objective of this work was to investigate the possible relevance of HP to platelet function. Methods: We characterized the profile of platelet activation in the presence of HP extracts through an evaluation of molecular markers by flow cytometry: mobilization of intracellular Ca++ and expression of platelet receptors such as activated GPIIbIIIa and P-selectin (CD62). Results: The results indicated a possible inhibitory effect of HP on the platelet activation response, which could be explained by the effect on intracellular calcium mobilization and the expression of activated GPIIbIIIa receptors. Despite of the limitations of an in vitro study, our results provide evidence of the possible mechanisms of action of HP. Conclusions: Further studies are needed to elucidate the effect of HP on hemostasis, but it may be recognized as a substance with antiplatelet properties.

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Section: Discussionmentioning

confidence: 99%

Hypericum perforatum and Its Potential Antiplatelet Effect

et al. 2022

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“…Hypericum perforatum L. plants were harvested at Alacam (Samsun, Turkey), during the first half of August 2018. The detailed procedure related to the preparation of plant extracts is described by MATIĆ et al (2021). Flowers were separated from blossoms before extraction.…”

Section: Preparation and Characterization Of Hypericum Perforatum L E...mentioning

confidence: 99%

“…Dried extracts were dissolved in DMSO at a stock concentration of 25 mg/mL, and stored at +4°C. The chemical characterization of H. perforatum extracts was performed by LC-MS analysis and 1 H-NMR, as reported previously (MATIĆ et al, 2021). The most abundant compounds: quercetin, I3, II8-Biapigenin, pseudohypericin, and adhyperforin were found only in hexane extracts of flowers and branch-body parts; hyperforin was found in hexane extracts of flowers and branch-body parts, ethyl-acetate of branch-body, and methanol extract of flowers; hypericin was found in methanol and hexane extracts of flowers and branch-body (MATIĆ et al, 2021).…”

Section: Preparation and Characterization Of Hypericum Perforatum L E...mentioning

confidence: 99%

“…Cancer cell lines were maintained in RPMI-1640 nutrient medium enriched with 10% fetal bovine serum, 2 mM L-glutamine, 25 mM HEPES, and a solution of penicillinstreptomycin (100 U/mL of penicillin and 0.1 mg/mL of streptomycin), as described elsewhere (MATIĆ et al, 2021). PC-3 (5000 cells per well) and DU 145 (5000 cells per well) were placed in 96-well microtiter plates, and the next day the cells were treated with five different concentrations of the extracts (ranging from 3.125 µg/mL to 50 µg/mL, or from 6.25 µg/mL to 100 µg/mL or from 12.5 µg/mL to 200 or 400 µg/mL, depending on the intensity of the cytotoxic activity of the particular extract against specific cell line).…”

Section: Determination Of Cytotoxic Activitymentioning

confidence: 99%

“…The amount and abundance of active substances and secondary metabolites depend on the geography and season of plant harvesting (MENEGAZZI et al, 2021). In our previous study, we have shown that H. perforatum extracts of various plant parts and obtained by different extraction solvents contain different compounds such as quercetin, pseudohypericin, adhyperforin, hypericin, and biapigenin (MATIĆ et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

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Hypericum perforatum L. extracts exert cytotoxic effects and show different miRNA signatures in PC-3 and DU 145 prostate cancer cells

et al. 2022

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Phytochemicals and bioactive substances derived from a wide range of plant extracts have been reported to exert various anticancer effects. Prostate cancer is one of the leading causes of cancer-related deaths within the male population. Prostate cancer-specific miRNA signatures were associated with cancer formation and progression, with various subtypes, and response to therapy. MicroRNA levels of expression were shown to change after the treatment of various compounds and substances extracted from natural products. Natural herbal compounds were shown to induce variations in miRNA expression levels in cancer cells. The aims of this study were to investigate the cytotoxic effects of methanol, ethyl-acetate, and hexane extracts obtained from branch-body part and flowers of Hypericum perforatum L. against humane PC-3 and DU 145 and to test potential miRNA-128/133b/155/193a/206/21/335 signature changes and differences between the two prostate cancer cell lines. Cytotoxic activity of H. perforatum extracts, their effects on cell cycle distribution, and miRNA expression levels were examined in humane PC-3 and DU 145 prostate cancer cells by MTT cell survival assay, flow cytometry, and quantitative real-time PCR. Hexane extract of flowers showed the strongest intensity of cytotoxic activity against PC-3 and DU 145 cells. The highest increase in the percentage of PC-3 cells in the subG1 phase was observed in cell samples treated with hexane extract of flowers and branch-body part. Significant differences in miRNA-128/133b/155/193a/206/21/335 levels were observed between PC-3 and DU 145 cell lines, especially in samples treated with flower extracts compared with the branch-body part. Conclusions: Investigated extracts have significant anticancer potential not only from the aspects of cytotoxicity and cell cycle effects but also from the aspect of lowering oncogenic or increasing tumor-suppressive miRNAs. The best effect might be the increase of tumor-suppressive miR-128 (accompanied by miR-193a) induced by the hexane extract of the flowers, which also exerted the highest cytotoxic activity. Hexane extract of flowers may be the candidate for further investigation for improving the efficiency of standard therapies for PCa. A miRNA signature might be cell-type specific after the treatment with H. perforatum extracts.

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