Cytotoxic Activity Evaluation of Eriocaulon Cinereum R.BR. On Hela and Vero Cell Lines

Abstract

Objective: This study aimed to evaluate the cytotoxic activity of the extracts and fractions of Eriocaulon cinereum against HeLa and Vero cell lines,which represent cervical cancer and normal cells, respectively. In addition, a phytochemical screening was carried out to determine the chemicalconstituents in the extracts and the active fractions.Materials and Methods: The extracts of E. cinereum were obtained by ultrasound-assisted extraction method using n-hexane, ethyl acetate, andmethanol, successively. The active extract was fractionated using vacuum liquid chromatography with dichloromethane followed by ethyl acetate. Thecytotoxic activity was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay method and was measured using microplatereader at the wavelength 595 nm. The data were analyzed with PROBIT from SPSS 16 for Windows®. In addition, phytochemical screening wasperformed using standard procedures.Results: The cytotoxic evaluation of the extracts of E. cinereum showed that the ethyl acetate extract was the most active extract against HeLa cell linewith the half maximal inhibitory concentration (IC50) value of 580.07 μg/ml. The dichloromethane and ethyl acetate fractions from the active extractof E. cinereum exhibited cytotoxic activity against HeLa cell with the IC50 values of 466.61 μg/ml and 267.34 μg/ml, respectively. In addition, the ethylacetate fraction showed a low cytotoxic effect against Vero cell line. The phytochemical screening of the ethyl acetate fraction indicated the presenceof terpenoids and alkaloids.Conclusion: This finding revealed the anticancer potential of E. cinereum and warranted further investigation for the discovery of new anticanceragents from natural resources for cervical cancer.

Cytotoxic activity of flavonoid from local plant Eriocaulon cinereum R.B against MCF-7 breast cancer cells

“…Based on the literature, this plant can be developed as an anticancer agent with minimal toxicity. [ 2 3 4 ] Information about E. cinereum is still minimal compared to other species of the genus. With many similarities to E. cinereum , Eriocaulon sieboldianum was used as supportive therapy for cancer treatment in China.…”

Anticancer Activity of Subfractions from Eriocaulon cinereum R.Br Extract in Cervical Cancer Cells

“…These results follow previous studies of MCF-7 or T47D cells, in line with the hypothesis proposed in this study. [20][21][22][23][24][25][26] The compound selectivity index, which was also determined in this study, showed that the selectivity of compounds in normal Vero cells was 2.157, which is classified as being in a good category with a value >2. This shows that the compound is selective and can differentiate between cancer cells and normal cells.…”

“…23,24 However, research using this species on HeLa cervical cancer cells has only been done at the fraction level, with the best IC50 being in the ethyl acetate fraction with an IC50 of 235.65 μg/mL. 25,26 Based on previous research, we hypothesized that the subfraction and isolates from this fraction would have better activity than the crude extract on HeLa…”