Cytotoxic Activity of Luvunga Scandens Against Human Cancer Cell Lines

Abstract

Luvunga scandens belongs to the family of Rutaceae which usually inhabit tropical and moist environment. This plant is known as ‘Mengkurat Jakun’ among locals and used traditionally to treat fever and fatigue via decoction. The aim of this study was to investigate the cytotoxic activity of the leaves and stems extracts of L. scandens extract. Extracts of the leaves and stems were obtained from sequential extraction procedures by various organic solvents. All extracts were subjected to cytotoxic study by 3-(4, 5-dimethylthaizol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. In in vitro cytotoxicity assay, all L. scandens extracts exhibited cytotoxicity against human breast adenocarcinoma (MCF-7) and human lung adenocarcinoma (A549) cell lines. The IC50 values of dichloromethane and methanol extracts from the leaves of L. scandens against MCF-7 cell line were 62.5 µg/mL and 88.0 µg/mL, respectively, whereas IC50 of methanol extract from stem was 81.0 µg/mL. All extracts were less active against A549 cell line where IC50 value were not be determined. The present findings revealed the potential of L. scandens as a cytotoxic agent against MCF-7 cell line. However, further studies should be planned to evaluate role of the plant in cytotoxic activity.

Preliminary Phytochemical Screening, In Vitro Antidiabetic, Antioxidant Activities, and Toxicity of Leaf Extracts of Psychotria malayana Jack

“…The methanol and water extracts of P. malayana leaves were tested for their cytotoxicity effect by determining the metabolic activity of 3T3-L1-treated cells compared to the negative control (untreated 3T3-L1 cells) via the MTT (3-(4,5-Dimethylthiazole-2-Yl)-2,5-Diphenyltetrazolium Bromide) assay according to the method reported by Al-Zikri et al [67] with some modifications. A total of 1.0-2.0 × 10 5 cells/mL were seeded in a 96-well plate overnight at 37 • C with a 5% CO 2 atmosphere.…”

Cytotoxic Activities of Extracts and Isolated Compounds of Some Potential Sumatran Medicinal Plants against MCF-7 and HSC-3 Cell Lines

“…Cytotoxic activity was measured by MTT assay following the protocol developed by Mosmann [15], with a slight modification [16]. The extracts and isolated compounds were dissolved in DMSO and serially diluted in DMEM to obtain different concentrations (3.125, 6.25, 12.5, 25, 50 and 100 µg/mL).…”

“…These isolated compounds, together with methanolic extracts of some Sumatran medicinal plants traditionally used as complementary agents for cancer treatment, i.e. rhizomes of A. denticulata, A. malaccensis, A. submutica, and C. zedoaria, aerial parts of C. asiatia, leaves of M. indica and P. crocatum, petals of M. frondosa and eight isolated compounds previously isolated from some Sumatran medicinal plants; crocatin A (2) and crocatin B (3) [8], curcumin (4) [9], demethoxymatteucinol (5) [10], mangiferin (7) [11], methyl caffeate (8) [12], tiliroside (10) [13] and usnic acid (11) [14], were tested for their cytotoxic activity towards MCF-7 human breast adenocarcinoma cell lines and HSC-3 human oral squamous cell carcinoma cell lines by MTT assay (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) as described in [15], with a slight modification [16]. The structures of all isolated compounds (1-11) are shown in Figure 1.…”

Analysis of phytochemical constituents and antioxidant activity from the fractions of Luvunga sarmentosa root extract using LCMS/MS

“…Luvunga is a genus of the family Rutaceae (Al-Zikri et al 2016). Luvunga sarmentosa is an accepted name based on the POWO.…”

“…Studies on several species of the genus Luvunga have been carried out. The stem and leave extract of Luvunga scandens has a cytotoxic activity (Al-Zikri et al 2016), antibacterial activity (Van et al 2020), and an aphrodisiac (Fauzi and Widodo 2019). The phytochemical content of several species of the genus Luvunga has been reported.…”