Cytotoxic aggregates of α-lactalbumin induced by unsaturated fatty acid induce apoptosis in tumor cells

Zhang, Min; Yang, Fang; Chen, Jie; Zheng, Chengchao; Liang, Yi

doi:10.1016/j.cbi.2009.03.019

Cited by 67 publications

(46 citation statements)

References 43 publications

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“…It is also suggested that the oligomeric state of HAMLET is fragile and easily dissociated by 2 M urea. The fragile nature of the oligomeric ␣-LA-OA complex was also reported in BAMLET: (i) The oligomeric BAMLET complex was dissociated by dilution and the subsequent gel filtration (30), and (ii) the major signals observed in the mass spectrometric analysis of originally aggregated BAMLET were reported to be monomeric under physiological conditions (32). Furthermore, the anti-tumor complex originally prepared from human milk was reported to be oligomeric (33), supporting our conclusion that HAMLET is oligomeric under physiological conditions.…”

Section: Discussionmentioning

confidence: 99%

Molecular Mechanisms of the Cytotoxicity of Human α-Lactalbumin Made Lethal to Tumor Cells (HAMLET) and Other Protein-Oleic Acid Complexes

Nakamura

Aizawa

Kariya

et al. 2013

Journal of Biological Chemistry

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Background:The ␣-lactalbumin-oleic acid complex has a unique apoptotic activity for selectively killing tumor cells. Results: We identified the oleic acid-binding site in the human-and goat-␣-lactalbumin complexes by two-dimensional NMR. Conclusion: Oleic acid is bound to a loosely organized hydrophobic core of the proteins in the molten globule state. Significance: The results are crucial for understanding the molecular mechanisms of the cytotoxicity of the protein-oleic acid complexes.

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Section: Discussionmentioning

confidence: 99%

Molecular Mechanisms of the Cytotoxicity of Human α-Lactalbumin Made Lethal to Tumor Cells (HAMLET) and Other Protein-Oleic Acid Complexes

Nakamura

Aizawa

Kariya

et al. 2013

Journal of Biological Chemistry

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“…In particular in human breast milk it was shown that the α-lactalbumin-oleic acid complex called HAMLET (Human Alpha-lactalbumin Made LEthal to Tumor cells) is able to induce tumor-selective apoptosis. This complex may be considered as a potential therapeutic agent against various tumor cells [43,44]. Other authors [45] have demonstrated the anti-inflammatory activity of α-lactalbumin through the inhibition of cyclooxygenase-2 (COX-2) and phospholipase A 2 activities.…”

Section: Characterization Of Donkey Milk Protein Fraction By Twodimenmentioning

confidence: 99%

A Proteomic Study on Donkey Milk

Vincenzetti¹,

Amici²

2012

Biochem Anal Biochem

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In children with Cow Milk Protein Allergy (CMPA), when it is not possible to breast feed or to use cow milk, the clinical use of donkey milk is considered since several studies have demonstrated the high similarity of donkey milk compared to human milk.An analysis was performed on donkey milk protein profile by two-dimensional electrophoresis (2-DE) followed by N-terminal sequencing in order to give a panoramic view of the proteins that are present in donkey milk. Furthermore, the interest was focused on the casein fractions and on their phosphorylation degree that may influence the calcium binding ability of caseins. At this purpose experiments on donkey milk casein dephosphorylation have been performed and the dephosphorylated casein fractions have been identified after 2-DE analysis followed by N-terminal sequencing. Among caseins were found mainly αs1-and β-caseins that showed a considerable heterogeneity due to variable degree of phosphorylation and to the presence of genetic variants. Finally, a quantitative determination of some antimicrobial proteins, such as lactoferrin and lactoperoxidase, that could be able to stimulate the development of the neonatal intestine, was performed in donkey milk, with the results being 0.080±0.0035 g/L and 0.11±0.027 mg/L, respectively. From the obtained data is evinced that human and donkey milk contain considerable amounts of lysozyme and lactoferrin but lactoperoxidase is present only in small amounts, confirming the high similarity between donkey and human milk. The present study on donkey milk proteins may be useful to assess the nutritional characteristics of this milk that is used to feed children affected by CMPA, but also may open the possibility of utilizing donkey milk in the general population to benefit subjects with CMPA, such as adults and the elderly.

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“…These include OA-conditioned anion exchange chromatography using either a DEAE-Trisacryl M in the conventional method [3,5,6,19] or a DEAE-Sepharose column in an alternative method [53]. Direct mixing under heat denaturation [30,36,52], heat denaturation of -LA and OA-conditioned anion exchange chromatography [62], direct mixing in solution at room temperature with alternated pH conditions [45,48,49,63], direct mixing followed by anion exchange chromatography [61] and alkaline conditions [64] have also been used.…”

Section: Methods Of Hamlet or Hamlet-analog Preparation In Vitromentioning

confidence: 99%

“…Regardless of the preparation method used in the different experiments, it has been shown that the cytotoxicity of the resulting complexes was similar to that of conventionally prepared HAMLET or BAMLET [30,36,48,49,54,[61][62][63][64][65].…”

Section: Methods Of Hamlet or Hamlet-analog Preparation In Vitromentioning

confidence: 99%