Cytotoxic and antibacterial activity of the mixture of olive oil and lime cream in vitro conditions

Abstract

The mixture of olive oil and lime cream has been traditionally used to treat external burns in the region of Hatay/Antakya and middle Anatolia. Olive oil and lime cream have been employed by many physicians to treat many ailments in the past. A limited number of studies have shown the antibacterial effect of olive oil and that it does not have any toxic effect on the skin. But we did not find any reported studies on the mixture of olive oil and lime cream. The aim of this paper is to investigate the cytotoxic and antibacterial activity of olive oil and lime cream individually or/and in combination in vitro conditions, by using disk-diffusion method and in cell culture. The main purpose in using this mixture is usually to clear burns without a trace. Agar overlay, MTT (Cytotoxicity assay) and antibacterial susceptibility tests were used to investigate the cytotoxic and antibacterial activity of olive oil and lime cream. We found that lime cream has an antibacterial activity but also cytotoxic on the fibroblasts. On the other hand olive oil has limited or no antibacterial effect and it has little or no cytotoxic on the fibroblasts. When we combined lime cream and olive oil, olive oil reduced its cytotoxic impact. These results suggest that mixture of olive oil and lime cream is not cytotoxic and has antimicrobial activity.

Knowledge, Attitudes, and Practices toward First Aid Management of Skin Burns in Saudi Arabia

“…While several studies have postulated mechanisms for which traditional remedies may be beneficial, there is a paucity of evidence to support their use, and clinical reports are often anecdotal. Some groups have attempted to evaluate the pharmacological properties and mechanisms of action of a number of traditional remedies, including SulconaW, a traditional Siddha medicine originating from Southern India [ 22 ], and the mixture of olive oil and lime cream originating from Turkey [ 23 ]. The Amish community has been observed using traditional remedies such as a combination of burns and wounds ointment (typically aloe vera, lanolin, honey, white oak bark, comfrey root, and lobelia) with burdock leaves [ 24 ].…”

Antibacterial Activity Test Turmeric(Curcuma longa L.) Extract Herbal Oil in Extra Virgin Olive Oil Against Staphylococcus aureus and Propionibacterium acnes

“…The antibacterial activity test of single extract of turmeric and single extract of olive oil in this study resulted in inhibition zones respectively are 4.80 mm and 3.33 mm. When compared with the results of a study on the antibacterial activity test of a single extract of olive oil, lime cream and a combination of lime cream and olive oil, a 1:1 ratio of S. aureus bacteria resulted in inhibition zones of 8 mm, 9 mm and 9 mm, respectively [41].…”

Response to: Practice of first aid in burn related injuries in a developing country

“…Oils were recommended in the first aid management of burn wounds in the 1900s, before the belief that this makes subsequent treatment more difficult [5]. Some groups have attempted to evaluate the pharmacological properties and mechanisms of action of a number of traditional remedies including Sulcona W , a traditional Siddha medicine originating from Southern India [6], and the mixture of olive oil and lime cream originating from Turkey [7]. The Amish community have been observed using traditional remedies such as a combination of burns and wounds ointment (typically aloe vera, lanolin, honey, white oak bark, comfrey root and lobelia) with burdock leaves [8].…”