Cytotoxic and antibacterial effect of a red propolis mouthwash, with or without fluoride, on the growth of a cariogenic biofilm

Martins, Mariana Leonel; Monteiro, Amanda Souza Nunes; Guimarães, Juli Emily Costa; Guimarães, Maria Bárbara de Carvalho Torres; Silva, Raphael Ferreira da; Cabral, Lúcio Mendes; Farah, Adriana; DePaula, Juliana; Romanos, Maria Teresa Villela; Maia, Lucianne Cople; Cavalcanti, Yuri Wanderley; Fonseca‐Gonçalves, Andréa

doi:10.1016/j.archoralbio.2019.104512

Cited by 31 publications

(19 citation statements)

References 35 publications

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“…The obtained results confirm a previous report on green Brazilian propolis with a strong growth inhibition of S. mutans , Streptococcus sanguinis , and P. gingivalis [ 19 ]. Our results are similar to a recent report on red propolis, where antibacterial activity against oral streptococci and lactobacilli was tested [ 20 ]. In the present study, the EEPs were highly active against C. albicans ATCC 76615 (MICs below 10 mg/mL of EEP each).…”

Section: Discussionsupporting

confidence: 92%

“…In the present study, an activity of EEP already in low concentrations was seen on the preformed cariogenic biofilm. The results are similar to a recent study on red Brazilian propolis, where the activity of 0.8% propolis was similar to those of 0.12% CHX; it is of interest to note that the addition of 500 ppm sodium fluoride did not affect the antibiofilm activity of that EEP [ 20 ]. Different results were found on a six-species biofilm consisting, among others, of S. mutans and C. albicans ; a mouth rinse containing 10% propolis did not have any effect, whereas a reduction was seen after applying a toothpaste with 0.9% propolis [ 38 ].…”

Section: Discussionsupporting

confidence: 89%

“…[ 17 ]. In vitro, antimicrobial and anti-biofilm activity by selected propolis were shown [ 18 , 19 , 20 ].…”

Section: Introductionmentioning

confidence: 99%

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In Vitro Activity of Propolis on Oral Microorganisms and Biofilms

et al. 2021

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Natural products are being discussed as alternatives to commonly used chemicals in antimicrobial therapy. The study aimed to investigate the antimicrobial activity of propolis against microbial species associated with caries, periodontal disease, and Candida infections. Two commercially available ethanolic extracts of Brazilian and one of European propolis (EEP) were used. The minimal inhibitory concentrations (MIC) of propolis and controls against eight microbial strains were determined. Scanning and transmission electron microscopy (SEM and TEM) images visualized the effect of propolis on microorganisms. Subsequently, the activity on three different multi-species biofilms (both formation and existing biofilms) was assessed. All MIC values of the Brazilian EEPs were low against the tested oral species (≤ 0.1 mg/mL–3.13 mg/mL propolis (Candida albicans)). The European EEP had slightly higher MICs than the Brazilian EEPs. The SEM and TEM images suggest an interaction of propolis with the microbial cell wall. The European EEP exhibited the strongest effect on retarding biofilm formation, whereas the Brazilian EEPs were highly active against preformed biofilms (100 mg/mL propolis of both EEPs reduced colony forming unit counts always by more than 6 log10). The antimicrobial and anti-biofilm activities point to the potential of propolis as an adjunct in oral health care products.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 89%

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In Vitro Activity of Propolis on Oral Microorganisms and Biofilms

et al. 2021

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“…According to several authors, extracts and fractions obtained from BRP are highly useful to reduce the formation of mono and multispecies biofilms when using in vitro and in vivo biofilm models [26,27,29,33] . From all the studies reported, the anti‐biofilm potential of BRP against bacteria responsible for oral diseases are very well established, and this bee product has been considered as a promising natural agent for the development of oral care products [29,33,34] …”

Section: Resultsmentioning

confidence: 99%

Green and Red Brazilian Propolis: Antimicrobial Potential and Anti‐Virulence against ATCC and Clinically Isolated Multidrug‐Resistant Bacteria

Silva

Braun

et al. 2021

Chemistry & Biodiversity

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Brazilian green and red propolis stand out as commercial products for different medical applications. In this article, we report the antimicrobial activities of the hydroalcoholic extracts of green (EGP) and red (ERP) propolis, as well as guttiferone E plus xanthochymol (8) and oblongifolin B (9) from red propolis, against multidrug‐resistant bacteria (MDRB). We undertook the minimal inhibitory (MIC) and bactericidal (MBC) concentrations, inhibition of biofilm formation (MICB50), catalase, coagulase, DNase, lipase, and hemolysin assays, along with molecular docking simulations. ERP was more effective by displaying MIC and MBC values <100 μg mL−1. Compounds 8 and 9 displayed the lowest MIC values (0.98 to 31.25 μg mL−1) against all tested Gram‐positive MDRB. They also inhibited the biofilm formation of S. aureus (ATCC 43300 and clinical isolate) and S. epidermidis (ATCC 14990 and clinical isolate), with MICB50 values between 1.56 and 6.25 μg mL−1. The molecular docking results indicated that 8 and 9 might interact with the catalase's amino acids. Compounds 8 and 9 have great antimicrobial potential.

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“…In the present study, regular use of mouthwash did not show any signi cant impact on the peri-implant microbiome. It is known that mouthwash affects the supragingival bio lm (66)(67)(68). However, it is unclear which kind of affect a mouthwash with common rinsing solutions has on the submucosal bio lm of implants.…”

Section: Discussionmentioning

confidence: 99%

Microbiological findings in early and late implant loss: an observational clinical case-controlled study

Korsch¹,

Marten

Stoll

et al. 2021

Preprint

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Background Implants are a predictable and well-established treatment method in dentistry. Nevertheless, looking at possible failures of dental implants, early and late loss have to be distinguished. The intent of the study was to report microbiological findings on the surface of implants with severe peri-implantitis, which had to be explanted. Methods 53 specimens of implants from 48 patients without severe general illnesses have been examined. The groups investigated were implants that had to be removed in the period of osseointegration (early loss, 13 patients with 14 implants) or after the healing period (late loss, 14 patients with 17 implants). The implant losses were compared with two control groups (implants with no bone loss directly after completed osseointegration, two to four months after implant placement (17 patients with 17 implants) and implants with no bone loss and prosthetic restoration for more than three years (5 patients with 5 implants)). Data about the bacteria located in the peri-implant sulcus was collected using amplification and high throughput sequencing of the 16S rRNA gene. Results The biofilm composition differed substantially between individuals. Both in early and late implant loss of Fusobacterium nucleatum , and Porphyromonas gingivalis were found to be abundant . Late lost implants showed higher bacterial diversity and in addition higher abundances of Treponema, Fretibacterium, Pseudoramibacter and Desulfobulbus , while microbial communities of early loss implants were very heterogeneous and showed no significantly more abundant bacterial taxa. Conclusions Specific peri-implant pathogens were found around implants that were lost after a primarily uneventful osseointegration. P. gingivalis and F. nulceatum frequently colonized the implant in early and late losses and could therefore be characteristic for implant loss in general. In general, early lost implants showed also lower microbial diversity than late losses. However, the microbial results were not indicative of the causes of early and late losses.

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Cytotoxic and antibacterial effect of a red propolis mouthwash, with or without fluoride, on the growth of a cariogenic biofilm

Cited by 31 publications

References 35 publications

In Vitro Activity of Propolis on Oral Microorganisms and Biofilms

In Vitro Activity of Propolis on Oral Microorganisms and Biofilms

Green and Red Brazilian Propolis: Antimicrobial Potential and Anti‐Virulence against ATCC and Clinically Isolated Multidrug‐Resistant Bacteria

Microbiological findings in early and late implant loss: an observational clinical case-controlled study

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