Cytotoxic and Antifungal Activities of 5-Hydroxyramulosin, a Compound Produced by an Endophytic Fungus Isolated fromCinnamomum mollisimum

Abstract

An endophytic fungus isolated from the plant Cinnamomum mollissimum was investigated for the bioactivity of its metabolites. The fungus, similar to a Phoma sp., was cultured in potato dextrose broth for two weeks, followed by extraction with ethyl acetate. The crude extract obtained was fractionated by high-performance liquid chromatography. Both crude extract and fractions were assayed for cytotoxicity against P388 murine leukemic cells and inhibition of bacterial and fungal pathogens. The bioactive extract fraction was purified further and characterized by nuclear magnetic resonance, mass spectral and X-ray crystallography analysis. A polyketide compound, 5-hydroxyramulosin, was identified as the constituent of the bioactive fungal extract fraction. This compound inhibited the fungal pathogen Aspergillus niger (IC50 1.56 μg/mL) and was cytotoxic against murine leukemia cells (IC50 2.10 μg/mL). 5-Hydroxyramulosin was the major compound produced by the endophytic fungus. This research suggests that fungal endophytes are a good source of bioactive metabolites which have potential applications in medicine.

Molecular profiling and bioactive potential of an endophytic fungus Aspergillus sulphureus isolated from Sida acuta : a medicinal plant

“…Correspondingly, an endophytic fungus similar to Phoma sp. isolated from Cinnamomum mollissimum offered potent cytotoxicity against murine leucemia cells (Santiago et al 2012). Also, the ethyl acetate extract of endophytic fungi isolated from P. crocatum and red alga Bostrychia tenella inhibited the growth of different tumour cell lines with IC 50 value of 37.43 (T47D) and 1 lg/mL (SF-295), respectively (Astuti et al 2014;de Felicio et al 2015).…”

Fungal Endophytes: An Amazing and Hidden Source of Cytotoxic Compounds

“…The compound was cytotoxic against murine leukemia cells with an IC 50 of 2.10 μg/mL (Santiago et al 2012).…”

Pyrophen Produced by Endophytic Fungi Aspergillus sp Isolated from Piper crocatum Ruiz & Pav Exhibits Cytotoxic Activity and Induces S Phase Arrest in T47D Breast Cancer Cells

“…Exploration of endophytic fungi have gained increased interest among scientist as these microorganisms produce biologically-active compounds with therapeutic values (Zhao et al, 2009;Santiago et al, 2012;do Nascimento et al, 2015;Richardson et al, 2015). Many of the compounds have been reported to produce bioactive compounds against various types of cancer cell lines (Chui et al, 2011;Zheng et al, 2013;Zhu et al, 2013;Chen et al, 2015;Wang et al, 2015 …”