Cytotoxic and apoptotic effects of single and mixed oxides of β-sitosterol on HepG2-cells

Koschutnig, Karin; Heikkinen, Susanna; Kemmo, Suvi; Lampi, Anna‐Maija; Piironen, Vieno; Wagner, Karl‐Heinz

doi:10.1016/j.tiv.2009.03.007

Cited by 45 publications

(34 citation statements)

References 31 publications

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“…Moreover, a number of phytochemicals were isolated as active components for anti-inflammatory activity in VS seeds. Investigation of present study verified that LnA, LA, and oleanolic acids exhibit significant antiinflammatory activities, whereas phytochemicals such as 7-ketositosterol, stigmasterol-glc, and soyasaponin 1 show relatively weak inhibitory activities, which are consistent with the observations of other investigators [Koschutnig et al, 2009;Puangpraphant and de Mejia, 2009;Reutrakul et al, 2010;Zha et al, 2011].…”

Section: Discussionsupporting

confidence: 92%

“…LnA and phytochemicals such as oleanolic acids, 7-ketositosterol, stigmasterol-glc, and soyasaponin 1 have been previously isolated from various plants, and they were shown to exert inhibitory activities [Ren and Chung, 2007;Koschutnig et al, 2009;Puangpraphant and de Mejia, 2009;Reutrakul et al, 2010;Zha et al, 2011]. Thus, focus was placed on the analysis of anti-inflammatory activity of LA.…”

Section: Resultsmentioning

confidence: 99%

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Anti-inflammatory effects of cowpea (Vigna sinensis K.) seed extracts and its bioactive compounds

Lee

Cui

et al. 2011

J Korean Soc Appl Biol Chem

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Anti-inflammatory effects of methanol extract and solvent fractions of cowpea (Vigna sinensis K.; VS) seeds and the isolated compounds were evaluated. Ethyl acetate and n-butanol fractions of VS seeds were found to strongly inhibit nitric oxide (NO) production, and inducible nitric oxide synthase (iNOS) mRNA and protein expressions in lipopolysaccharides (LPS)-stimulated RAW264.7 macrophage cells. Compounds inhibiting NO production in RAW264.7 cells were isolated and identified via successive partitioning and spectroscopic characterization. The active compounds included oleanolic acid, linolenic acid (LnA), linoleic acid (LA), 7-ketositosterol, stigmasterol-glucose (glc), and soyasaponin 1. Among the isolated compounds, LnA and LA were found to inhibit NO production significantly. Contents of LnA and LA in VS seeds were 2.034 and 1.162 mg/g on dry weight basis, respectively. Reverse transcriptase-polymerase chain reaction (RT-PCR) and Western blot analysis show inhibitory effects of these compounds. LnA and LA repressed mRNA and protein levels of iNOS in LPS-stimulated cells. LA suppressed the production of pro-inflammatory cytokines such as interleukin (IL)-1β, IL-6, and tumor necrosis factor (TNF)-α in LPS-induced macrophage cells. Thus, VS was found to exhibit strong antiinflammatory activity, which may be partly attributed to the polyunsaturated fatty acids such as LnA and LA.

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Section: Discussionsupporting

confidence: 92%

Section: Resultsmentioning

confidence: 99%

Anti-inflammatory effects of cowpea (Vigna sinensis K.) seed extracts and its bioactive compounds

Lee

Cui

et al. 2011

J Korean Soc Appl Biol Chem

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“…19 Antioxidant, cytotoxic and antibacterial effects of fatty acids, steroids were confirmed previously. [25][26][27][28][29][30][31] Likewise, some polycyclic aromatic hydrocarbons and terpenoids have been reported for these bioactive properties. [32][33][34][35] Therefore, the potential biological efficacy of DCM extract may be related to the combination of these compounds and their synergies correlation with each other.…”

Section: Resultsmentioning

confidence: 99%

Bioactive Properties of Eremostachys macrophylla Montbr. & Auch. Rhizomes Growing in Iran

Asgharian¹,

Delazar²,

Lotfipour³

et al. 2017

Pharm Sci

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“…Human Hepatoma (HepG2 and its derivatives) cells are successfully introduced in humans in order to study cellular metabolism and its cytotoxic and genotoxic responses to contact with determined materials [23,24] . The implantability test defines the material's behavior in vivo, the tissue response and the device performance.…”

Section: Introductionmentioning

confidence: 99%

Analysis of Poly(Lactic-co-Glycolic Acid)/Poly(Isoprene) Polymeric Blend for Application as Biomaterial

Marques¹,

Santos²,

Schopf³

et al. 2013

Polímeros

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Abstract:The application of renewable raw materials encourages research in the biopolymers area. The Poly(Lacticco-Glycolic Acid)/Poly(Isoprene) (PLGA/IR) blend combines biocompatibility for application in the health field with excellent mechanical properties. The blend was obtained by solubilization of polymers in organic solvents. To investigate the polymer thermochemical properties, FTIR and DSC were applied. To investigate the composition's influence over polymer mechanical properties, tensile and hardness test were applied. To analyze the blends response in the cell environment, a stent was produced by injection molding process, and Cell Viability Test and Previous Implantability were used. The Infrared spectra show that chemical composition is related only with polymers proportion in the blend. The calorimetry shows a partial miscibility in the blend. The tensile test shows that adding Poly(Isoprene) to Poly(Lactic-co-Glycolic Acid) induced a relevant reduction in the Young modulus, tensile stress and tenacity of the material, which was altered from the fragile raw PLGA to a ductile material. The composition did not affect the blend hardness. The cell viability test shows that the blend has potential application as biomaterial, while the first results of implantability indicate that the polymeric stent kept its original position and caused low fibrosis.

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Cytotoxic and apoptotic effects of single and mixed oxides of β-sitosterol on HepG2-cells

Cited by 45 publications

References 31 publications

Anti-inflammatory effects of cowpea (Vigna sinensis K.) seed extracts and its bioactive compounds

Anti-inflammatory effects of cowpea (Vigna sinensis K.) seed extracts and its bioactive compounds

Bioactive Properties of Eremostachys macrophylla Montbr. & Auch. Rhizomes Growing in Iran

Analysis of Poly(Lactic-co-Glycolic Acid)/Poly(Isoprene) Polymeric Blend for Application as Biomaterial

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