Cytotoxic and genotoxic effects of ethanolic extract of Euphorbia hyssopifolia L. on HepG2 cells

Araújo, Silvany de Sousa; Fernandes, Thaís Cristina Casimiro; Tafurt-Cardona, Yaliana; Almeida, Pedro Marcos de; Marin-Morales, Maria Aparecida; Santos, Andréa V.; Randau, Karina Perrelli; Benko‐Iseppon, Ana Maria; Brasileiro-Vidal, Ana Christina

doi:10.1016/j.jep.2015.04.044

Cited by 15 publications

(5 citation statements)

References 10 publications

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“…Shi et al 2008, Mwine and Van Damme 2011), although some caution must be required due to the toxic potential of these plants (e.g. Araújo et al 2015). Euphorbia hirta and Euphorbia hyssopifolia are cosmopolitan spurges with widely known medicinal properties, standing out mainly due to their diuretic and antimicrobial activities (e.g.…”

Section: Introductionmentioning

confidence: 99%

Unraveling the karyotype structure of the spurges Euphorbia hirta Linnaeus, 1753 and E. hyssopifolia Linnaeus, 1753 (Euphorbiaceae) using genome size estimation and heterochromatin differentiation

Santana

Pinangé

Vasconcelos

et al. 2016

CCG

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Euphorbia Linnaeus, 1753 (Euphorbiaceae) is one of the most diverse and complex genera among the angiosperms, showing a huge diversity in morphologic traits and ecologic patterns. In order to improve the knowledge of the karyotype organization of Euphorbia hirta (2n = 18) and Euphorbia hyssopifolia (2n = 12), cytogenetic studies were performed by means of conventional staining with Giemsa, genome size estimations with flow cytometry, heterochromatin differentiation with chromomycin A3 (CMA) and 4’,6-diamidino-2-phenylindole (DAPI) and Giemsa C-banding, fluorescent in situ hybridization (FISH) with 45S and 5S rDNA probes, and impregnation with silver nitrate (AgNO3). Our results revealed small metacentric chromosomes, CMA+/DAPI0 heterochromatin in the pericentromeric regions of all chromosomes and CMA+/DAPI− in the distal part of chromosome arms carriers of nucleolar organizing regions (NORs). The DNA content measurements revealed small genomes for both species: Euphorbia hirta with 2C = 0.77 pg and Euphorbia hyssopifolia with 2C = 1.41 pg. After FISH procedures, Euphorbia hirta, and Euphorbia hyssopifolia presented three and four pairs of terminal 45S rDNA sites, respectively, colocalizing with CMA+ heterochromatic blocks, besides only one interstitial pair of 5S rDNA signals. Additionally, the maximum number of active NORs agreed with the total number of observed 45S rDNA sites. This work represents the first analysis using FISH in the subfamily Euphorbioideae, revealing a significant number of chromosomal markers, which may be very helpful to understand evolutionary patterns among Euphorbia species.

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Section: Introductionmentioning

confidence: 99%

Unraveling the karyotype structure of the spurges Euphorbia hirta Linnaeus, 1753 and E. hyssopifolia Linnaeus, 1753 (Euphorbiaceae) using genome size estimation and heterochromatin differentiation

Santana

Pinangé

Vasconcelos

et al. 2016

CCG

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“…Our COMET assay showed that the plant extract did not cause any deleterious effect on the cell DNA. Previous studies assessing the genotoxicity of medicinal plants showed great variability in their genotoxic potential, depending on the plants and types of extracts tested [45,46,47,48]. We demonstrated that P. mauritianum did not exert a high cytotoxicity regardless of the cell lines tested.…”

Section: Discussionmentioning

confidence: 52%

The Polyphenol-Rich Extract from Psiloxylon mauritianum, an Endemic Medicinal Plant from Reunion Island, Inhibits the Early Stages of Dengue and Zika Virus Infection

Clain

Haddad

Koishi

et al. 2019

IJMS

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The recent emergence and re-emergence of viral infections transmitted by vectors, such as the Zika virus (ZIKV) and Dengue virus (DENV), is a cause for international concern. These highly pathogenic arboviruses represent a serious health burden in tropical and subtropical areas of the world. Despite the high morbidity and mortality associated with these viral infections, antiviral therapies are missing. Medicinal plants have been widely used to treat various infectious diseases since millenaries. Several compounds extracted from plants exhibit potent effects against viruses in vitro, calling for further investigations regarding their efficacy as antiviral drugs. Here, we demonstrate that an extract from Psiloxylon mauritianum, an endemic medicinal plant from Reunion Island, inhibits the infection of ZIKV in vitro without exhibiting cytotoxic effects. The extract was active against different ZIKV African and Asian strains, including an epidemic one. Time-of-drug-addition assays revealed that the P. mauritianum extract interfered with the attachment of the viral particles to the host cells. Importantly, the P. mauritianum extract was also able to prevent the infection of human cells by four dengue virus serotypes. Due to its potency and ability to target ZIKV and DENV particles, P. mauritianum may be of value for identifying and characterizing antiviral compounds to fight medically-important flaviviruses.

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“…The comet assay is a technique used for detecting the presence of single strand breaks of DNA at alkali-sensitive sites or sites of incomplete excision repair in mammalian cells 8 . These tests have been used to analyze the toxicity 9 as well as antigenotoxic, anti-mutagenic and anti-carcinogenic effects 10,11 of extracts and isolated compounds from plants. The genotoxicity of anticancer drugs can result in secondary tumors in non tumor cells.…”

Section: Introductionmentioning

confidence: 99%

Chemoprotective Effect of Carwin Capsules against Cyclophosphamide Induced Genotoxicity by In Vivo Micronucleus Assay

Thakur¹,

Upmanyu

Saxena³

et al. 2022

J. Drug Delivery Ther.

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The protective effects of carwin capsules (CAR) against toxicity induced by cyclophosphamide (CYC) in mice were investigated using the micronucleus assay for anticlastogenic activity in mouse bone marrow cells. The experimental animals were pre-treated orally with the drug suspension (carwin capsule suspension) for seven consecutive days (drug alone in group III and in combination with standard drug in group IV). The standard drug (cyclophosphamide, 50 mg/kg bw) was dissolved in saline and injected intraperitoneally. The results demonstrate that carwin was found to be significant (P<0.05) as compared to cyclophosphamide treated as the no. of MNPCEs in carwin treated was decreased effectively. And also carwin was able to significantly (P<0.01) protect the action caused by cyclophosphamide as well was also found to be very effective antigenotoxic (P<0.01) as compared to cyclophosphamide in scoring of PCE: NCE ratio. All the groups were compared with cyclophosphamide (-ve control). So, it was concluded that the used polyherbal formulation (carwin) was found to be very effective drug in cancer therapy as it has very less genotoxic effect as compared to cyclophosphamide and also has a protective effect with cyclophosphamide. Keywords: Carwin capsules, Cyclophosphamide, Micronucleus assay, Antigenotoxic, Polyherbal formulation.

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Cytotoxic and genotoxic effects of ethanolic extract of Euphorbia hyssopifolia L. on HepG2 cells

Cited by 15 publications

References 10 publications

Unraveling the karyotype structure of the spurges Euphorbia hirta Linnaeus, 1753 and E. hyssopifolia Linnaeus, 1753 (Euphorbiaceae) using genome size estimation and heterochromatin differentiation

Unraveling the karyotype structure of the spurges Euphorbia hirta Linnaeus, 1753 and E. hyssopifolia Linnaeus, 1753 (Euphorbiaceae) using genome size estimation and heterochromatin differentiation

The Polyphenol-Rich Extract from Psiloxylon mauritianum, an Endemic Medicinal Plant from Reunion Island, Inhibits the Early Stages of Dengue and Zika Virus Infection

Chemoprotective Effect of Carwin Capsules against Cyclophosphamide Induced Genotoxicity by In Vivo Micronucleus Assay

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