Cytotoxic and genotoxic effects of in vitro exposure to Triclosan and Trimethoprim on zebra mussel (Dreissena polymorpha) hemocytes

Binelli, Andrea; Cogni, D.; Parolini, Marco; Riva, Consuelo; Provini, A.

doi:10.1016/j.cbpc.2009.02.005

Cited by 75 publications

(58 citation statements)

References 42 publications

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“…Only a few species of freshwater invertebrates have been studied using neutral red retention as a gauge of toxicity, the snails Vivparus contectus [43] and Lymnaea stagnalis [44], and the bivalves, Dreissena polymorpha [29,30], and Lamellidens marginalis [45]. Our use of a unionid bivalve, Elliptio complanata, and a planorbid snail, Helisoma trivolvis in the present study increases the number of freshwater invertebrate species tested, and both have a broad North American geographical distribution.…”

Section: Discussionmentioning

confidence: 99%

“…Since lysosomes accumulate a diverse range of toxic chemicals that can eventually lead to cell injury through lysosomal damage [34,35], destabilization of lysosomal membranes indicated by leakage of neutral red into the cytoplasm is a good indicator of stress. Lysosomal destabilization has been shown in oysters exposed to toxic algae [36] and to Fullerene C60 nanoparticles [24], in earthworms exposed to organophosphate pesticides [37], in clams carrying trematode parasites [38], in mussels exposed to alkylbenzene sulphonate [39], and to human pharmaceuticals [29,30,32,33]. The vast majority of these and other studies have utilized marine invertebrates even though freshwater molluscs are excellent sentinel organisms under constant pollution pressure.…”

Section: Introductionmentioning

confidence: 99%

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Toxic Effects of Copper, Cadmium, and Methoxychlor Shown by Neutral Red Retention Assay in Two Species of Freshwater Molluscs

Molnar

Fong²

2012

TOEPTJ

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Toxic Effects of Copper, Cadmium, and Methoxychlor Shown by Neutral Red Retention Assay in Two Species of Freshwater Molluscs

Molnar

Fong²

2012

TOEPTJ

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“…(27,(58)(59)(60)(61)(62)(63) compared with heavy metals (26,55,64). On the basis of the collected literature regarding the effects of heavy metals on the lysosomal membrane stability of mussels (and other mollusc species) we found that our results are similar with those of other authors and confirm that the lysosomal stability weakens with the increasing toxicant concentrations, and vice versa − the retention time of the dye is longer for the lower concentrations..…”

Section: Neutral Red Retention Timementioning

confidence: 99%

Lysosomal membrane stability and respiration rate in zebra mussel (Dreissena polymorpha) as biomarkers for ex situ heavy metal exposure

Yancheva¹

2017

Period. Biol.

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Lysosomal membrane stability and respiration rate in zebra mussel (Dreissena polymorpha Pallas, 1771) as biomarkers for ex situ heavy metal exposure Abstract Background and purpose: In the current study we aimed to investigate the lysosomal membrane stability in haemocytes of the invasive mollusk zebra mussel (Dreissena polymorpha Pallas, 1771) by applying the neutral red retention assay (NRRA), as well as changes in the respiration rate and survival under acute heavy metal exposure. (Pb) Materials and methods: The mussels were treated with different decreasing concentrations of nickel (Ni) and lead INTRODUCTION In recent years, heavy metal pollution in the environment and food has been considered as a global issue (1,2). Large quantities of heavy metals have been released into aquatic systems, both fresh and marine worldwide due to a global rapid population growth and intensive domestic activities, as well as expanding industrial and agricultural production (3,4). Thus, heavy metals have severely deteriorated the aquatic ecosystems because of their toxicity, abundance, persistence, and subsequent bioaccumulation and biomagnification (5).To monitor the health of aquatic systems, sentinel organisms such as mussels (bivalves) have been proposed to be suitable ''biomonitors'' of pollution as they accumulate heavy metals in their tissues and shells (6,7). Their filtering habits, low metabolism and ability to bioaccumulate pollutants make them an excellent choice to assess their bioavailability and effects (8-10). Mussels are also sessile, sedentary, have a reasonable size, they are worldwide distributed and often found in large amounts (11-13).Marine mussels such as Mytilus sp., have been most often addressed as sensitive indicators for monitoring toxic substances in water (14-17). However, other bivalve species belonging to different taxa, such as Dreissena polymorpha have also been successfully used in ecotoxicological studies and monitoring programs (18)(19)(20)(21)(22)(23)(24)(25)(26)(27).The immune defence of mussels is comprised of cellmediated and humoral mechanisms, in which the haemocytes or blood cells play a key role (28). They are also involved in the detoxification process through the accumulation of metallic and organic xenobiotics in their well-developed endolysosomal system (29). Lysosomes are highly conserved multi-functional cellular organelles present in almost all cells of eukaryotic organisms, from yeast to humans. Their function in the cellular economy includes the degradation of redundant or damaged organelles (e.g., mitochondria and endoplasmic reticulum) and longer lived proteins as part of the autophagic cellular turnover (30). Lysosomes are also involved in normal physiological responses such as the digestion of materials ingested by endocytosis and phagocytosis (i.e., intracellular digestion) (30,31). Moreover, the lysosomal system, which is remarkably well-developed in mussel haemocytes is well-known as a target site for toxic metals and organic chemicals, due to its ability to accum...

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“…Binelli et al, 62 evaluated cytotoxic and genotoxic effects of trimethoprim on zebra mussel hemocytes. In this study, the potential cellular damage was investigated by in vitro exposure.…”

Section: Structure Of Trimethoprimmentioning

confidence: 99%

Untitled

2012

IJPSR

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Cytotoxic and genotoxic effects of in vitro exposure to Triclosan and Trimethoprim on zebra mussel (Dreissena polymorpha) hemocytes

Cited by 75 publications

References 42 publications

Toxic Effects of Copper, Cadmium, and Methoxychlor Shown by Neutral Red Retention Assay in Two Species of Freshwater Molluscs

Toxic Effects of Copper, Cadmium, and Methoxychlor Shown by Neutral Red Retention Assay in Two Species of Freshwater Molluscs

Lysosomal membrane stability and respiration rate in zebra mussel (Dreissena polymorpha) as biomarkers for ex situ heavy metal exposure

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