Cytotoxic and Hepatoprotective Effects of Bupleurum flavum Flavonoids on Hepatocellular Carcinoma HEP-G2 Cells

Gevrenova, Reneta; Zheleva‐Dimitrova, Dimitrina; Ruseva, Silviya; Denkov, Nikolay; Konstantinov, Spiro; Lozanov, Valentin; Mitev, В.

doi:10.9734/bjpr/2016/25785

Cited by 5 publications

(2 citation statements)

References 27 publications

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“…In the same way, 6- C -hexosyl-luteolin was confirmed by the diagnostic ions 0,3 X − at m / z 357.061 (39.3%) and 0,2 X − at m / z 327.051 (53.7%); luteolin ([Lu-H] − at m / z 285.041) was deduced from RDA ions 1,3 B − at m / z 133.028 and 1,4 A − at m / z 175.038. Based on the comparison with reference standards, 62 and 63 were unambiguously identified as vicenin-2 [ 32 ] and homoorientin, respectively.…”

Section: Resultsmentioning

confidence: 99%

An In-Depth Study of Metabolite Profile and Biological Potential of Tanacetum balsamita L. (Costmary)

Gevrenova

Zengin

Sinan

et al. 2022

Plants

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Asteraceae species Tanacetum balsamita L. (costmary) is renowned for its traditional usage as an aromatic, carminative and tonic plant. This work aimed at in-depth study of the phytochemical and in vitro biological profilings of methanol–aqueous extracts from the costmary leaves, flower heads and roots. An UHPLC-HRMS analysis revealed more than 100 secondary metabolites including 24 acylquinic acids, 43 flavonoid glycosides, aglycones and methoxylated derivatives together with 15 phenolic acids glycosides. For the first time, 91 compounds are reported in the costmary. The flower heads extract possessing the highest content of total phenolics and flavonoids, actively scavenged DPPH (84.54 ± 3.35 mgTE/g) and ABTS radicals (96.35 ± 2.22 mgTE/g), and showed the highest reducing potential (151.20 and 93.22 mg TE/g for CUPRAC and FRAP, respectively). The leaves extract exhibited the highest inhibition towards acetyl- and butyrylcholinesterase (2.11 and 2.43 mg GALAE/g, respectively) and tyrosinase (54.65 mg KAE/g). The root extract inhibited α-glucosidase (0.71 ± 0.07 mmol ACAE/g), α-amylase (0.43 ± 0.02 mmol ACAE/g) and lipase (8.15 ± 1.00 mg OE/g). At a concentration >2 µg/mL, a significant dose dependent reduction of cell viability towards THP-1 monocyte leukemic cells was observed. Costmary could be recommended for raw material production with antioxidant and enzyme inhibitory properties.

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Section: Resultsmentioning

confidence: 99%

An In-Depth Study of Metabolite Profile and Biological Potential of Tanacetum balsamita L. (Costmary)

Gevrenova

Zengin

Sinan

et al. 2022

Plants

View full text Add to dashboard Cite

show abstract

“…In a previous study, Compound 2 did not show cytotoxic activity up to 100 µmol/L on the IGROV-1 and OVCAR-3 human ovary cell lines and HCT-116 human colon cancer cell line ( Znati et al, 2014 ). It was also determined that narcissin (25–400 μg/mL) had no significant hepatoprotective activity when tested on HEP-G2 hepatocellular carcinoma cells for 72 h by MTT assay ( Gevrenova et al, 2016 ). In the present study, the IC 50 values of Compound 2 were 137.10 ± 8.62 and 77.78 ± 39.88 μg/mL on the MCF-7 and MDA-MB-231 cell lines, respectively.…”

Section: Discussionmentioning

confidence: 99%