Cytotoxic Constituents of Propolis from Myanmar and Their Structure-Activity Relationship

Li, Feng; Awale, Suresh; Tezuka, Yasuhiro; Kadota, Shigetoshi

doi:10.1248/bpb.32.2075

Cited by 46 publications

(30 citation statements)

References 26 publications

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“…Other studies showed that propolis ethanolic extract is cytotoxic on pulp fibroblasts [30] and cancer cells [31]. In the concentrations tested in this study, the cytotoxicity of the varnish containing propolis was considered low.…”

Section: Discussionmentioning

confidence: 89%

Propolis Varnish: Antimicrobial Properties against Cariogenic Bacteria, Cytotoxicity, and Sustained-Release Profile

Luca

Franca

Macedo

et al. 2014

BioMed Research International

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Varnishes are preparations that differ in the polymeric matrix and therapeutical agents. In dentistry they are used to prevent caries. In this study we developed a propolis varnish, considering propolis properties against cariogenic bacteria. To a chitosan polymeric base (CHV) was added ethanolic propolis extract in different concentrations: PV1 (5%), PV2 (10%), and PV3 (15%). Antimicrobial activity was carried out against Streptococcus mutans (SM), Streptococcus sanguinis (SG), Streptococcus salivarius (SS), and Lactobacillus casei (LC) through agar diffusion method. The three propolis concentrations incorporated were effective in inhibiting the growth of all microorganisms, but without significant difference between the zones of inhibition observed. Cytotoxicity assay was done by MTT method. Data were analyzed by one-way ANOVA and Bonferroni test. None of the varnishes were cytotoxic, keeping 80% of viable cells, while CHV allowed cellular proliferation (120%). Sustained-release test was carried out by applying 40 μL of each varnish in the buccal surface of bovine teeth and kept in an ethanol/water solution removed in regular times. According to the “independent model approach,” the release profiles were distinct from each varnish and the most prolonged was PV3 (8 weeks). Varnish formulations had satisfactory antimicrobial activity against cariogenic bacteria and have a low cytotoxicity (<50%).

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Section: Discussionmentioning

confidence: 89%

Propolis Varnish: Antimicrobial Properties against Cariogenic Bacteria, Cytotoxicity, and Sustained-Release Profile

Luca

Franca

Macedo

et al. 2014

BioMed Research International

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“…Thirteen cycloartane-type triterpenes and four prenylated flavanones isolated from Myanmar propolis were evaluated. [60] A cycloartane-type triterpene, 3a,27-dihydroxycycloart-24E-en-26-oic acid, showed potent cytotoxicity against B16-BL6 murine melanoma cells, and (2S)-5,7-dihydroxy-4′-methoxy-8,3′-diprenylflavanone exhibited strong cytotoxic action against human cancer cell lines (lung A549 adenocarcinoma, cervix HeLa adenocarcinoma and HT-1080 fibrosarcoma). [60] A methanolic extract of propolis from Myanmar was found to inhibit PANC-1 human pancreatic cancer cells preferentially under nutrient-deprived conditions.…”

Section: Sh-sy5ymentioning

confidence: 99%

“…[60] A cycloartane-type triterpene, 3a,27-dihydroxycycloart-24E-en-26-oic acid, showed potent cytotoxicity against B16-BL6 murine melanoma cells, and (2S)-5,7-dihydroxy-4′-methoxy-8,3′-diprenylflavanone exhibited strong cytotoxic action against human cancer cell lines (lung A549 adenocarcinoma, cervix HeLa adenocarcinoma and HT-1080 fibrosarcoma). [60] A methanolic extract of propolis from Myanmar was found to inhibit PANC-1 human pancreatic cancer cells preferentially under nutrient-deprived conditions. Bioactivity-guided fractionation of the extract led to the isolation of cycloartanetype triterpenes (22Z,24E)-3-oxocycloart-22,24-dien-26-oic acid, which exhibited the most potent preferential cytotoxicity in a concentration-and time-dependent manner.…”

Section: Sh-sy5ymentioning

confidence: 99%

Cytotoxic constituents of propolis inducing anticancer effects: a review

Watanabe

Amarante

Conti

et al. 2011

Journal of Pharmacy and Pharmacology

190

133

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Objectives Propolis is a honeybee product used extensively in traditional medicine for its antioxidant, anti-inflammatory, immunomodulatory and anticancer effects. Propolis exhibits a broad spectrum of biological activities because it is a complex mixture of natural substances. In this review, the antitumour effects of propolis extracts and its constituents (e.g. flavonoids, terpenes and caffeic acid phenethyl ester) are discussed. Key findings The effect of propolis on experimental carcinogenesis is discussed, as well as its possible mechanisms of action against tumours, involving apoptosis, cell cycle arrest and interference on metabolic pathways. Propolis seems to be efficient against different tumour cells both in vitro and in vivo, which suggests its potential in the development of new anticancer drugs. Summary Propolis extracts may be important economically and would allow a relatively inexpensive cancer treatment. Preclinical investigations are needed to further elucidate the benefits of propolis and its antitumour properties.

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“…8-位具有异戊烯基、法呢烯基、lavandylyl 的黄酮在多药耐药 [mutidrug resistance protein 1 (MRP1)/ATP-binding cassette C1(ABCC1) inhibitor]中更具有潜力 [57] . 增加异戊烯基更是提高黄酮类化合物细胞毒活性的结构要求 [58] .…”

Section: 而黄酮醇的收率明显下降(Scheme 7)unclassified